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An open-air experiment was performed in Pistoia (Italy) to investigate the possible protective role played by different contents of UV-B absorbing compounds to realistic UV-B supplementation and to study its effect on plant fruit production. A mutant line and its normal counterpart of Lycopersicon esculentum Mill, which differ in the content of UV-B absorbing compounds, were used. Additional UV-B radiation in the field was supplied to simulate a 20% stratospheric ozone depletion. Two groups of plants were grown: ‘control’, where plants received only natural solar UV-B radiation, and ‘UV-B’ treatment, where plants were grown under supplemental UV-B. The results of the experiment showed that the content of UV-B absorbing compounds of treated plants did not differ from that of the control in both lines. This indicates that natural sunlight, in Mediterranean areas, is saturating for synthesis of these compounds also in plants with normal content of UV-B absorbing compounds. Consequently, plants are not able to produce significant additional amounts of them, in response to a realistic UV-B supplementation, in order to protect the plant from additional UV-B radiation. No different responses to the UV-B supplementation were found between the two lines. The most significant UV-B effect was an earlier reddening of fruits in comparison with the ‘control’ accompanied by a reduction in the size of mature fruits. No significant effects of UV-B treatment were observed in biomass accumulation, leaf ontogeny, flowering or productivity. 相似文献
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Paola Pellegrini Angela Strambi Chiara Zipoli Maria H?gg-Olofsson Maria Buoncervello Stig Linder Angelo De Milito 《Autophagy》2014,10(4):562-571
Acidic pH is an important feature of tumor microenvironment and a major determinant of tumor progression. We reported that cancer cells upregulate autophagy as a survival mechanism to acidic stress. Inhibition of autophagy by administration of chloroquine (CQ) in combination anticancer therapies is currently evaluated in clinical trials. We observed in 3 different human cancer cell lines cultured at acidic pH that autophagic flux is not blocked by CQ. This was consistent with a complete resistance to CQ toxicity in cells cultured in acidic conditions. Conversely, the autophagy-inhibiting activity of Lys-01, a novel CQ derivative, was still detectable at low pH. The lack of CQ activity was likely dependent on a dramatically reduced cellular uptake at acidic pH. Using cell lines stably adapted to chronic acidosis we could confirm that CQ lack of activity was merely caused by acidic pH. Moreover, unlike CQ, Lys-01 was able to kill low pH-adapted cell lines, although higher concentrations were required as compared with cells cultured at normal pH conditions. Notably, buffering medium pH in low pH-adapted cell lines reverted CQ resistance. In vivo analysis of tumors treated with CQ showed that accumulation of strong LC3 signals was observed only in normoxic areas but not in hypoxic/acidic regions. Our observations suggest that targeting autophagy in the tumor environment by CQ may be limited to well-perfused regions but not achieved in acidic regions, predicting possible limitations in efficacy of CQ in antitumor therapies. 相似文献
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During the last few decades many experiments have been performed to evaluate the responses of plants to enhanced solar UV-B radiation (280–320 nm) that may occur because of stratospheric ozone depletion; most of them were performed in controlled environment conditions where plants were exposed to low photosynthetically active radiation (PAR) levels and high UV-B irradiance. Since environmental radiative regimes can play a role in the response of plants to UV-B enhancement, it appears doubtful whether it is valid to extrapolate the results from these experiments to plants grown in natural conditions. The objective of this work was to evaluate the effects on physiology and morphology of a bean (Phaseolus vulgaris L.) cultivar Nano Bobis, exposed to supplemental UV radiation in the open-air. UV-B radiation was supplied by fluorescent lamps to simulate a 20% stratospheric ozone reduction. Three groups of plants were grown: control (no supplemental UV), UV-A treatment (supplementation in the UV-A band) and UV-B treatment (supplemental UV-B and UV-A radiation). Each group was replicated three times. After 33 days of treatment plants grown under UV-B treatment had lower biomass, leaf area and reduced leaf elongation compared to UV-A treatment. No significant differences were detected in photosynthetic parameters, photosynthetic pigments and UV-B absorbing compounds among the three groups of plants. However, plants exposed to UV-A treatment showed a sort of 'stimulation' of their growth when compared to the control. The results of this experiment showed that plants may be sensitive to UV-A radiation, thus it is difficult to evaluate the specific effects of UV-B (280–320 nm) radiation from fluorescent lamps and it is important to choose the appropriate control. Environmental conditions strongly affect plant response to UV radiation so further field studies are necessary to assess the interaction between UV-B exposure and meteorological variability. 相似文献
4.
UV responses of Lolium perenne raised along a latitudinal gradient across Europe: a filtration study
Comont D Martinez Abaigar J Albert A Aphalo P Causton DR Figueroa FL Gaberscik A Llorens L Hauser MT Jansen MA Kardefelt M de la Coba Luque P Neubert S Núñez-Olivera E Olsen J Robson M Schreiner M Sommaruga R Strid A Torre S Turunen M Veljovic-Jovanovic S Verdaguer D Vidovic M Wagner J Winkler JB Zipoli G Gwynn-Jones D 《Physiologia plantarum》2012,145(4):604-618
Lolium perenne (cv. AberDart) was grown at 14 locations along a latitudinal gradient across Europe (37-68°N) to study the impact of ultraviolet radiation (UV) and climate on aboveground growth and foliar UV-B absorbing compounds. At each location, plants were grown outdoors for 5 weeks in a replicated UV-B filtration experiment consisting of open, UV-B transparent (cellulose diacetate) and UV-B opaque (polyester) environments. Fourier transform-infrared spectroscopy was used to compare plant metabolite profiles in relation to treatment and location. UV radiation and climatic parameters were determined for each location from online sources and the data were assessed using a combination of anova and multiple regression analyses. Most of the variation in growth between the locations was attributable to the combination of climatic parameters, with minimum temperature identified as an important growth constraint. However, no single environmental parameter could consistently account for the variability in plant growth. Concentrations of foliar UV-B absorbing compounds showed a positive trend with solar UV across the latitudinal gradient; however, this relationship was not consistent in all treatments. The most striking experimental outcome from this study was the effect of presence or absence of filtration frames on UV-absorbing compounds. Overall, the study demonstrates the value of an European approach in studying the impacts of natural UV across a large latitudinal gradient. We have shown the feasibility of coordinated UV filtration at multiple sites but have also highlighted the need for open controls and careful interpretation of plant responses. 相似文献
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Grifoni D Carreras G Zipoli G Sabatini F Dalla Marta A Orlandini S 《International journal of biometeorology》2008,52(8):755-763
Besides playing an essential role in plant photosynthesis, solar radiation is also involved in many other important biological
processes. In particular, it has been demonstrated that ultraviolet (UV) solar radiation plays a relevant role in grapevines
(Vitis vinifera) in the production of certain important chemical compounds directly responsible for yield and wine quality. Moreover, the
exposure to UV-B radiation (280–320 nm) can affect plant–disease interaction by influencing the behaviour of both pathogen
and host. The main objective of this research was to characterise the solar radiative regime of a vineyard, in terms of photosynthetically
active radiation (PAR) and UV components. In this analysis, solar spectral UV irradiance components, broadband UV (280–400 nm),
spectral UV-B and UV-A (320–400 nm), the biological effective UVBE, as well as the PAR (400–700 nm) component, were all considered.
The diurnal patterns of these quantities and the UV-B/PAR and UV-B/UV-A ratios were analysed to investigate the effect of
row orientation of the vineyard in combination with solar azimuth and elevation angles. The distribution of PAR and UV irradiance
at various heights of the vertical sides of the rows was also studied. The results showed that the highest portion of plants
received higher levels of daily radiation, especially the UV-B component. Row orientation of the vines had a pronounced effect
on the global PAR received by the two sides of the rows and, to a lesser extent, UV-A and UV-B. When only the diffused component
was considered, this geometrical effect was greatly attenuated. UV-B/PAR and UV-A/PAR ratios were also affected, with potential
consequences on physiological processes. Because of the high diffusive capacity of the UV-B radiation, the UV-B/PAR ratio
was significantly lower on the plant portions exposed to full sunlight than on those in the shade. 相似文献
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Mediterranean beaches are very crowded during summer and, because of the high values of solar UV radiation, the potential risk for human health is relevant. In this study, all-day measurements of biologically effective global and diffuse UV radiation for skin (UVBEeryt) and eye (UVBEpker, UVBEpconj, UVBEcat) disorders were carried out on differently tilted surfaces on a summers day on a Mediterranean beach. The role played by beach umbrellas in protection from excessive sun exposure was also investigated. Erythema, photokeratitis and cataract seem to require almost the same exposure time to reach the risk threshold dose. Under full sunlight, the highest global and diffuse UV values are reached on surfaces normally oriented towards sunlight and on horizontal surfaces, respectively. Over vertical surfaces, at this northern hemisphere site, global and diffuse UV radiation reaches maxima values in the south-facing direction around noon, while maxima values are reached early in the morning and late in the afternoon over surfaces facing east and west, respectively. The quality of the beach umbrellas protection (efficiency in blocking solar UV radiation) varies with surface orientation; the highest efficiency for our specific site and geometrical conditions occurs over horizontal surfaces, with efficiency being least over vertical surfaces when incident radiation values are still relevant. 相似文献
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Anna Dalla Marta D. Grifoni M. Mancini G. Zipoli S. Orlandini 《International journal of biometeorology》2011,55(1):87-96
Climate and meteorological conditions greatly affect agricultural activities, modifying plant responses and determining the
quantity and the quality of production. In this respect, the aim of this research was to analyze the quality of winter durum
wheat (Triticum turgidum L. var. durum), in terms of protein content through the use of meteorological information. Meteorological conditions were described utilizing
both local weather station data (air temperature, cumulated precipitation) and large-scale information available freely on
the internet, such as geopotential height (GPH), sea surface temperature (SST), and the North Atlantic Oscillation (NAO) index.
The analysis was carried out for the period 1997–2009 in the Tuscany region, Central Italy. Grain protein was positively correlated
with air temperature during the February to June period, and negatively with cumulative precipitation during the entire period
from November to June. Protein content was also negatively correlated with 500 hPa GPH over Gibraltar and North-Western Africa
during the March to June period and with the SST of the Atlantic Ocean south-west of the Canary Islands during the January
to June period. Finally, with regard to the NAO, winter durum wheat quality was positively correlated with the specific index
for several months, in particular during the winter period. These results demonstrate that precipitation and air temperature
over the production area represent two crucial variables driving the vegeto-productive responses of winter durum wheat. On
the other hand, the use of large-scale meteorological information showed great potential from the perspective of a local quality
forecast system setup. 相似文献
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