全文获取类型
收费全文 | 150篇 |
免费 | 19篇 |
出版年
2017年 | 2篇 |
2015年 | 2篇 |
2014年 | 2篇 |
2013年 | 25篇 |
2012年 | 3篇 |
2011年 | 5篇 |
2010年 | 6篇 |
2009年 | 7篇 |
2008年 | 6篇 |
2007年 | 6篇 |
2006年 | 2篇 |
2005年 | 10篇 |
2004年 | 5篇 |
2002年 | 5篇 |
2001年 | 5篇 |
1999年 | 4篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 6篇 |
1992年 | 3篇 |
1989年 | 2篇 |
1975年 | 2篇 |
1971年 | 1篇 |
1948年 | 1篇 |
1943年 | 1篇 |
1929年 | 1篇 |
1926年 | 1篇 |
1925年 | 1篇 |
1924年 | 1篇 |
1921年 | 1篇 |
1920年 | 1篇 |
1915年 | 2篇 |
1914年 | 1篇 |
1909年 | 1篇 |
1902年 | 1篇 |
1901年 | 1篇 |
1897年 | 2篇 |
1892年 | 1篇 |
1891年 | 1篇 |
1889年 | 1篇 |
1888年 | 1篇 |
1887年 | 1篇 |
1883年 | 3篇 |
1882年 | 5篇 |
1881年 | 2篇 |
1880年 | 1篇 |
1879年 | 6篇 |
1878年 | 2篇 |
1877年 | 2篇 |
1876年 | 1篇 |
排序方式: 共有169条查询结果,搜索用时 15 毫秒
1.
2.
L. Zavalova S. Lukyanov I. Baskova E. Snezhkov S. Akopov S. Berezhnoy E. Bogdanova E. Barsova E. D. Sverdlov 《Molecular & general genetics : MGG》1996,253(1-2):20-25
We previously detected in salivary gland secretions of the medicinal leech (Hirudo medicinalis) a novel enzymatic activity, endo-ɛ(γ-Glu)-Lys isopeptidase, which cleaves isopeptide bonds formed by transglutaminase (Factor
XIIIa) between glutamine γ-carboxamide and the ɛ-amino group of lysine. Such isopeptide bonds, either within or between protein
polypeptide chains are formed in many biological processes. However, before we started our work no enzymes were known to be
capable of specifically splitting isopeptide bonds in proteins. The isopeptidase activity we detected was specific for isopeptide
bonds. The enzyme was termed destabilase. Here we report the first purification of destabilase, part of its amino acid sequence,
isolation and sequencing of two related cDNAs derived from the gene family that encodes destabilase proteins, and the detection
of isopeptidase activity encoded by one of these cDNAs cloned in a baculovirus expression vector. The deduced mature protein
products of these cDNAs contain 115 and 116 amino acid residues, including 14 highly conserved Cys residues, and are formed
from precursors containing specific leader peptides. No homologous sequences were found in public databases.
Received: 9 April 1996 / Accepted: 17 May 1996 相似文献
3.
4.
5.
The medicinal leech Hirudo medicinalis produces various types of proteinase inhibitors: bdellins (inhibitors of trypsin, plasmin, and acrosin), hirustasin (inhibitor of tissue kallikrein, trypsin, -chymotrypsin, and granulocyte cathepsin G), tryptase inhibitor, eglins (inhibitors of -chymotrypsin, subtilisin, and chymasin and the granulocyte proteinases elastase and cathepsin G), inhibitor of factor Xa, hirudin (thrombin inhibitor), inhibitor of carboxypeptidase, and inhibitor of complement component C1s. This review summarizes data on their primary and tertiary structures, action mechanisms, and biological activities. 相似文献
6.
Tushmalova NA Pragina LL Baskova IP Basanova AV Zavalova LL 《Zhurnal vysshe? nervno? deiatelnosti imeni I P Pavlova》2001,51(2):252-253
In the paper are presented the results of the study of Pyavit, a complex leech preparation from, whose effects on the memory may be dependent on DNA methylation (one of the mechanisms of gene expression regulation). A positive effect of Pyavit on formation and retention of passive avoidance conditioned reaction was demonstrated in rat experiments. 相似文献
7.
Chalisova NI Pennijajnen VP Baskova IP Zavalova LL Bazanova AV 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2001,87(12):1650-1655
Components of leech (Hirudo medicinalis) salivary gland secretion (high molecular mass bdelin B, bdellastasin, eglin C, destabilize) were used to investigate their neurotrophic effect on organotypic culture of dorsal root ganglia of the 10-11-day old chicken embryos. Destabilize and high molecular mass bdelin B in concentrations of 0.01, 0.02, 0.05 and 0.1 ng/ml, bdellastasin in concentrations of 0.02 and 0.05 ng/ml, eglin C in concentrations of 0.1 ng/ml exhibit neurite-stimulating effect as compared to the control. 相似文献
8.
I. P. Baskova T. G. Yudina L. L. Zavalova A. S. Dudkina 《Biochemistry. Biokhimii?a》2010,75(5):585-589
The relative location of proteins and lipids in particles of medicinal leech salivary gland secretion (SGS) is revealed for
the first time. Their sizes and morphology are described. Using scanning electron microscopy and transmission electron microscopy,
it was determined that SGS consists of particles of different sizes and form. This picture is supported by confocal laser
scanning microscopy of SGS preparations treated with fluorescein isothiocyanate. After incubation with nonionic detergents
(Brij 35 and Tween 20), transmission electron microscopy revealed the dissociation of fragments composing protein-lipid particles
(PLP), and in this case an increase in free protein concentration determined by a modification of the Lowry method was observed.
Perylene probing of lipids in SGS preparations showed that they are concentrated mainly inside PLP and are almost absent on
the surface. Cholesterol was detected during SGS probing using the cholesteryl-Bodipy (hydrophobic fluorescent analog of cholesterol)
on surface sections during confocal analysis of electron microphotographs of SGS. This analysis detected PLP structures in
SGS resembling caveoles full of cholesterol. SGS, preliminary frozen at −70°C, transformed into a multitude of similar small
particles visualized by transmission electron microscopy, whose fixed distribution resembled water crystal structure. 相似文献
9.
Slizhikova DK Zinov'eva MV Kuz'min DV Snezhkov EB Shakhparonov MI Dmitriev RI Antipova NV Zavalova LL Sverdlov ED 《Molekuliarnaia biologiia》2007,41(4):659-665
Secretory polymeric immunoglobulins (IgA dimers and IgM pentamers) are unique in that, apart from L- and H-chains, they contain J-chains responsible for their oligomerization. These antibodies are part of the local adaptive immune system acting on mucosa membranes of the respiratory and digestive systems as the first protection barrier to potential infectious agents. Secretory polymeric immunoglobulins are produced by highly specific B-cells and actively transported to the surface of mucosa membrane through epithelium cells. Therefore, their synthesis and J-chain content are dependent upon epithelium translocation function and condition that are markedly affected by tumorous transformation. Here, we used RT-PCR and immunoblotting to study of the J-chain content and its mRNA expression level in normal and tumorous tissues in lung squamous cell cancer and adenocarcinoma at various stages of disease progression. 相似文献
10.