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Staphylococcus aureus is an important agent of contagious bovine intramammary infections in dairy cattle. Its ability to persist inside the udder is based on the presence of important mechanisms such as its ability to form biofilms, polysaccharide capsules small colony variants, and their ability to invade professional and nonprofessional cells, which will protect S. aureus from the innate and adaptive immune response of the cow, and from antibiotics that are no longer considered to be sufficient against S. aureus bovine mastitis. In this review, we present the recent research outlining S. aureus persistence properties inside the mammary gland, including its regulation mechanisms, and we highlight alternative therapeutic strategies that were tested against S. aureus isolated from bovine mastitis such as the use of probiotic bacteria, bacteriocins and bacteriophages. Overall, the persistence of S. aureus inside the mammary gland remains a pressing veterinary problem. A thorough understanding of staphylococcal persistence mechanisms will elucidate novel ways that can help in the identification of novel treatments. 相似文献
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Caroline Schmutz Alison Cartwright Helen Williams Oliver Haworth John HH Williams Andrew Filer Mike Salmon Christopher D Buckley Jim Middleton 《Arthritis research & therapy》2010,12(4):R161
Introduction
Monocytes/macrophages accumulate in the rheumatoid (RA) synovium where they play a central role in inflammation and joint destruction. Identification of molecules involved in their accumulation and differentiation is important to inform therapeutic strategies. This study investigated the expression and function of chemokine receptor CCR9 in the peripheral blood (PB) and synovium of RA, non-RA patients and healthy volunteers. 相似文献5.
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Jamil M Neto Marina GM Viturino Galina Ananina Flvia F Bajano Sueli M da S Costa Alicia B Roque Gessica FS Borges Raissa Franchi Priscila HH Rim Flvio M Medina Fernando F Costa Mnica B de Melo Jos PC de Vasconcellos 《Experimental biology and medicine (Maywood, N.J.)》2021,246(21):2290
This study aimed to investigate the association among genetic variants of the complement pathway CFB R32Q (rs641153), C3 R102G (rs2230199), and CFH (rs1410996) with age-related macular degeneration (AMD) in a sample of the Brazilian population. In a case-control study, 484 AMD patients were classified according to the clinical age-related maculopathy grading system (CARMS) and compared to 479 unrelated controls. The genetic variants rs1410996 of complement H (CFH), rs641153 of complement factor B (CFB), and rs2230199 of complement 3 (C3) were evaluated through polymerase chain reaction (PCR) and direct sequencing. The associations between single nucleotide polymorphisms (SNPs) and AMD, adjusted by age, were assessed by using logistic regression models. A statistically significant association was observed between AMD risk and rs2230199 variant with an OR of 2.01 (P = 0.0002) for CG individuals compared to CC individuals. Regarding the comparison of advanced AMD versus the control group, the OR was 2.12 (P = 0.0036) for GG versus AA genotypes for rs1410996 variant. Similarly, the OR for rs2230199 polymorphism was 2.3034 (P = 5.47e-05) when comparing CG individuals to CC carriers. In contrast, the rs641153 variant showed a significant protective effect against advanced AMD for GA versus GG genotype (OR = 0.4406; P = 0.0019). When comparing wet AMD versus controls, a significant association was detected for rs1410996 variant (OR = 2.16; P = 0.0039) comparing carriers of the homozygous GG versus AA genotype, as well as in the comparisons of GG (OR = 3.0713; P = 0.0046) and CG genotypes (OR = 2.2249; P = 0.0002) versus CC genotype for rs2230199 variant, respectively. The rs641153 variant granted a significant protective effect against wet AMD for GA versus GG genotypes (OR = 0.4601; P = 0.0044). Our study confirmed the risk association between rs2230199 and rs1410996 variants and AMD, and the protective role against AMD for rs641153 variant. 相似文献
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Prenylated flavonoids of Erythrina lysistemon grown in Egypt 总被引:1,自引:0,他引:1
Three prenylated flavonoid derivatives; 5,7,4′-trihydroxy-8-(3-methylbut-2-enyl)-6-(2″-hydroxy-3″-methylbut-3″enyl) isoflavone (isoerysenegalensein E), 5,7,2′-trihydroxy-4′-methoxy-5′-(3″-methylbut-2″-enyl) isoflavanone (lysisteisoflavanone), 5, 4′-dihydroxy-6-(3-methylbut-2-enyl)-2″-hydroxyisopropyl dihydrofurano [4″,5″:8,7] isoflavone (isosenegalensin), together with the four known flavonoids abyssinone V-4′-methylether, alpinumisoflavone, wighteone and burttinone were isolated from the stem bark of Erythrina lysistemon Hutch. (Leguminosae). Structures were elucidated by spectroscopic methods. 相似文献
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Three antibodies that recognize distinct fucose epitopes were used to study
fucosylation during growth and development of Dictyostelium discoideum.
mAb83.5 is known to recognize an undefined "fucose epitope" on several
proteins with serine-rich domains, while mAb CAB4, and a component of
anti-horse-radish peroxidase, specifically recognize Fucalpha1,6GlcNAc and
Fucalpha1,3GlcNAc residues respectively in the core of N-linked
oligosaccharides. We show that mAb 83.5 defines a new type of
O-glycosylation. Serine-containing peptides incubated with GDPbeta[3H]Fuc
and microsomes formed two fucosylated products. A neutral product
accounting for 30% of the label did not react with the antibody, while the
rest of the label was incorporated into a charged product which contained
all the mAb83.5 reactive material. beta- Elimination of the labeled peptide
or endogenous products produced [3H]Fuc-1-P, indicating phosphodiester
linkage to serine. Fucbeta-1-P and GDP-betaFuc at 100 microM blocked
mAb83.5 binding to endogenous and peptide products, but their alpha-linked
anomers did not. Electrospray ionization mass spectra of the neutral and
anionic labeled products showed major peaks of mass units corresponding to
O-Fuc-Ser peptide and O-Fuc-phospho-Ser peptide, respectively. The activity
of Fuc- phosphotransferase exactly paralleled the accumulation of reactive
glycans during growth and development. The expressions of N-glycan core
Fucalpha1,6GlcNAc and Fucalpha1,3GlcNAc and their respective fucosyl
transferase activities were also synchronous, but their developmental
regulation differed from one another. Fucalpha1, 6GlcNAc was expressed
maximally during growth but declined during development. In contrast core
Fucalpha1,3GlcNAc epitopes were expressed almost exclusively during
development. These findings provide direct evidence for a novel type of
O-phosphofucosylation, demonstrate the existence of an O- fucosyl
transferase, and identify two different types of core fucosylation in the
N-glycans of Dictyostelium.
相似文献
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Niels HH Heegaard Martin R Larsen Terri Muncrief Allan Wiik Peter Roepstorff 《Arthritis research & therapy》2000,2(5):407-8
The antigenic specificity of an unusual antinuclear antibody pattern in three patient sera was identified after separating HeLa-cell nuclear extracts by two-dimensional (2D) gel electrophoresis and localizing the antigens by immunoblotting with patient serum. Protein spots were excised from the 2D gel and their contents were analyzed by matrix-assisted laser desorption-ionization (MALDI) or nanoelectrospray ionization time-of-flight (TOF) tandem mass spectrometry (MS) after in-gel digestion with trypsin. A database search identified the proteins as the C1 and C2 heterogeneous nuclear ribonucleoproteins. The clinical spectrum of patients with these autoantibodies includes arthritis, psoriasis, myositis, and scleroderma. None of 59 patients with rheumatoid arthritis, 19 with polymyositis, 33 with scleroderma, and 10 with psoriatic arthritis had similar antibodies. High-resolution protein-separation methods and mass-spectrometric peptide mapping in combination with database searches are powerful tools in the identification of novel autoantigen specificities. 相似文献