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S. Ercan F. Ertugrul Y. Aydin F. S. Akfirat S. Hasancebi K. Akan Z. Mert N. Bolat O. Yorgancilar A. Altinkut-Uncuoglu 《Biologia Plantarum》2010,54(4):691-696
Expressed sequenced tags containing simple sequence repeats (EST-SSRs) were used to identify molecular markers associated
with yellow rust resistance in wheat (Triticum aestivum L.). A cross between yellow rust resistant (PI178383) and susceptible (Harmankaya99) wheat genotypes was performed and respective
DNA pools from the resistant and susceptible F2 seedlings were constructed. 78 EST-SSR primers were used for bulked segregant analysis and one EST-SSR marker (Pk54), identified as 200 bp fragment, was present in the resistant parent and resistant F2 hybrids but not in the susceptible ones. 108 wheat genotypes differing in yellow rust resistance were screened with Pk54 and 68 % of the wheat genotypes, known to be yellow rust resistant, had the Pk54 marker, further suggesting that the presence of this marker correlates with yellow rust resistance. 相似文献
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TDZ-specific plant regeneration in salad burnet 总被引:5,自引:0,他引:5
Various explants of salad burnet (Poterium sanguisorba L.=Sanguisorba minor Scop.) were cultured on semi-solid MS or B5 media containing factorial combinations of various plant growth regulators. Hypocotyl
and petiole explants, after initial callus stage, regenerated prolific adventitious shoots via organogenesis, when placed
on Murashige and Skoog basal medium containing 1–2 μM α-naphthaleneacetic acid and 4-20 μM thidiazuron. Any other growth regulator
combination tested failed to respond. The addition of the biocide, Plant Preservative Mixture, was effective in controlling
contamination and did not impair regeneration. Elongated shoots at 2–4 cm were transferred to rooting medium containing semi-solid
Murashige and Skoog plus 1 μM α-naphthaleneacetic and rooted plants were transferred to the glasshouse. This is the first
report on in vitro plant regeneration within the genusPoterium.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Semiha Erisen Mustafa Yorgancilar Emine Atalay Mehmet Babaoglu 《Plant Cell, Tissue and Organ Culture》2010,100(2):229-233
Prolific shoot regeneration via organogenesis was induced from leaf and leaf petiole explants of the endemic Astragalus cariensis species on Murashige and Skoog (MS) medium with α-naphthaleneacetic acid (NAA) and benzyladenine (BA) within 8 week. The
highest number of shoots (23/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. Elongated
shoots were successfully rooted in MS medium with 0.5 mg/l indole-3-butyric acid. Rooted plantlets were acclimatized in pots
containing 1:1 mixture of peat and perlite. 相似文献
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