全文获取类型
收费全文 | 26501篇 |
免费 | 2444篇 |
国内免费 | 2483篇 |
出版年
2024年 | 43篇 |
2023年 | 323篇 |
2022年 | 411篇 |
2021年 | 1252篇 |
2020年 | 928篇 |
2019年 | 1168篇 |
2018年 | 1076篇 |
2017年 | 864篇 |
2016年 | 1164篇 |
2015年 | 1723篇 |
2014年 | 1952篇 |
2013年 | 2143篇 |
2012年 | 2528篇 |
2011年 | 2284篇 |
2010年 | 1415篇 |
2009年 | 1285篇 |
2008年 | 1490篇 |
2007年 | 1322篇 |
2006年 | 1182篇 |
2005年 | 1033篇 |
2004年 | 936篇 |
2003年 | 887篇 |
2002年 | 766篇 |
2001年 | 497篇 |
2000年 | 431篇 |
1999年 | 356篇 |
1998年 | 207篇 |
1997年 | 200篇 |
1996年 | 186篇 |
1995年 | 138篇 |
1994年 | 168篇 |
1993年 | 125篇 |
1992年 | 141篇 |
1991年 | 126篇 |
1990年 | 106篇 |
1989年 | 86篇 |
1988年 | 77篇 |
1987年 | 70篇 |
1986年 | 54篇 |
1985年 | 71篇 |
1984年 | 35篇 |
1983年 | 36篇 |
1982年 | 27篇 |
1981年 | 15篇 |
1979年 | 15篇 |
1978年 | 17篇 |
1975年 | 7篇 |
1974年 | 7篇 |
1972年 | 13篇 |
1971年 | 8篇 |
排序方式: 共有10000条查询结果,搜索用时 78 毫秒
1.
Plant somatic cells have the capability to switch their cell fates from differentiated to undifferentiated status under proper
culture conditions, which is designated as totipotency. As a result, plant cells can easily regenerate new tissues or organs
from a wide variety of explants. However, the mechanism by which plant cells have such remarkable regeneration ability is
still largely unknown. In this study, we used a set of meristem-specific marker genes to analyze the patterns of stem cell
differentiation in the processes of somatic embryogenesis as well as shoot or root organogenesis in vitro. Our studies furnish preliminary and important information on the patterns of the de novo stem cell differentiation during various types of in vitro organogenesis. 相似文献
2.
A recombinant cell line (NIH3T3:pLtkSN) was made by infecting parental cells (NIH3T3) with a recombinant retrovirus (pLtkSN) encoding herpes simplex virus thymidine kinase (HSVtk) gene. The cells expressing HSVtk (NIH3T3:pLtkSN) grew 2.3 times more than the parental cells (NIH3T3) in Dulbecco's Modified Eagles Media containing 10% (v/v) horse serum. The NIH3T3:pLtkSN cells also showed a significant enhancement in the maximal cell concentration and the specific growth rate even at 2.5% serum concentration. The specific O2 uptake rate of NIH3T3 was 2.1 times greater than that of NIH3T3:pLtkSN. Under both O2-limited and O2-unlimited conditions, it appears that HSVtk plays an important role in enhancing the growth characteristics of animal cells. 相似文献
3.
We released seeds of two sympatric tree species, Corylus mandshurica (seed with thinner seed hull, higher nutrition) and C. heterophylla (seeds with thicker seed hull, lower nutrition) in the masting year of C. mandshurica in 2008, and C. heterophylla in 2009, respectively, to investigate how seed masting of the two sympatric Corylus species affects seed removal and dispersal fitness of the two species differently at both intra- and inter-specific levels.
At intra-specific level, the authors found mast seeding of both C. mandshurica and C. heterophylla significantly reduced seed removal, seed consumption, but increased seed dispersal distance and seed dispersal fitness of
the released seeds. Mast seeding of C. mandshurica increased seed caching of C. mandshurica. At inter-specific level, the authors found mast seeding of C. mandshurica reduced seed removal of C. heterophylla, but mast seeding of C. heterophylla did not significantly reduce seed removal of C. mandshurica. Mast seeding of C. mandshurica reduced seed consumption of C. heterophylla, while mast seeding of C. heterophylla reduced seed consumption of C. mandshurica. We found mast seeding of C. mandshurica significantly reduced seed dispersal distance of C. heterophylla, while mast seeding of C. heterophylla significantly increased seed dispersal distance of C. mandshurica. We found that mast seeding of C. mandshurica significantly increased seed dispersal fitness of C. heterophylla, while mast seeding of C. heterophylla did not significantly increase seed dispersal fitness of C. mandshurica. More studies are needed to reveal the ecological consequences of mast seeding at inter-specific or community-level. Seed
traits may attribute the differences of mast seeding at inter-specific level. Because seeds with thinner seed hull and higher
nutrition were more harvested and eaten by rodents, mast seeding of C. mandshurica might have reduced seed removal and seed consumption, but increased dispersal fitness of C. heterophylla (seeds with thicker seed hull, lower nutrition). Therefore, synchrony among species is, or is not, selectively beneficial
to the focus species depends on seed traits which determine gains from mast seeding at inter-specific level. 相似文献
4.
5.
Peng Chen Pranjal Swarup Wojciech Michal Matkowski Adams Wai Kin Kong Su Han Zhihe Zhang Hou Rong 《Ecology and evolution》2020,10(7):3561-3573
- As a highly endangered species, the giant panda (panda) has attracted significant attention in the past decades. Considerable efforts have been put on panda conservation and reproduction, offering the promising outcome of maintaining the population size of pandas. To evaluate the effectiveness of conservation and management strategies, recognizing individual pandas is critical. However, it remains a challenging task because the existing methods, such as traditional tracking method, discrimination method based on footprint identification, and molecular biology method, are invasive, inaccurate, expensive, or challenging to perform. The advances of imaging technologies have led to the wide applications of digital images and videos in panda conservation and management, which makes it possible for individual panda recognition in a noninvasive manner by using image‐based panda face recognition method.
- In recent years, deep learning has achieved great success in the field of computer vision and pattern recognition. For panda face recognition, a fully automatic deep learning algorithm which consists of a sequence of deep neural networks (DNNs) used for panda face detection, segmentation, alignment, and identity prediction is developed in this study. To develop and evaluate the algorithm, the largest panda image dataset containing 6,441 images from 218 different pandas, which is 39.78% of captive pandas in the world, is established.
- The algorithm achieved 96.27% accuracy in panda recognition and 100% accuracy in detection.
- This study shows that panda faces can be used for panda recognition. It enables the use of the cameras installed in their habitat for monitoring their population and behavior. This noninvasive approach is much more cost‐effective than the approaches used in the previous panda surveys.
6.
7.
8.
Jun‐juan Wang Qi‐min Zhang Wei‐wei Ni Xin Zhang Ying Li Ai‐li Li Peng Du Chun Li Su‐su Yu 《Microbiology and immunology》2019,63(8):303-315
We investigated the correlation between the beneficial effect of Lactobacillus acidophilus on gut microbiota composition, metabolic activities, and reducing cow's milk protein allergy. Mice sensitized with β‐lactoglobulin (β‐Lg) were treated with different doses of L. acidophilus KLDS 1.0738 for 4 weeks, starting 1 week before allergen induction. The results showed that intake of L. acidophilus significantly suppressed the hypersensitivity responses, together with increased fecal microbiota diversity and short‐chain fatty acids (SCFAs) concentration (including propionate, butyrate, isobutyrate, and isovalerate) when compared with the allergic group. Moreover, treatment with L. acidophilus induced the expression of SCFAs receptors, G‐protein–coupled receptors 41 (GPR41) and 43 (GPR43), in the spleen and colon of the allergic mice. Further analysis revealed that the GPR41 and GPR43 messenger RNA expression both positively correlated with the serum concentrations of transforming growth factor‐β and IFN‐γ (p < .05), but negatively with the serum concentrations of IL‐17, IL‐4, and IL‐6 in the L. acidophilus–treated group compared with the allergic group (p < .05). These results suggested that L. acidophilus protected against the development of allergic inflammation by improving the intestinal flora, as well as upregulating SCFAs and their receptors GPR41/43. 相似文献
9.
Zenghui Hu Yingbai Shen Fanyi Shen Youqing Luo Xiaohua Su 《Trees - Structure and Function》2009,23(5):1003-1011
Interplant communication has been widely demonstrated in plants, especially in herbaceous plants. In this study, mechanical
damage was shown to affect the levels of pyrochatechol, chlorogenic acid, gallic acid and p-hydroxyl benzoic acid in poplar (Populus simonii × P. pyramidalis ‘Opera 8277’) cuttings, indicating the activation of defense response. In neighboring intact cuttings, the levels of those
phenolics also varied when compared to the control, suggesting the interplant communication between poplar cuttings. Three
volatiles, methyl jasmonate, methyl salicylate and benzothiazole, were detected in volatiles emitted from mechanically damaged
poplar cuttings. All of them can induce changes in the levels of four phenolics. Therefore, they could act as airborne signals
between P. simonii × P. pyramidalis ‘Opera 8277’ cuttings. The different change patterns of phenolic contents induced by different volatiles imply that the defense
response activated in neighboring plants may be regulated by multiple signaling pathways. The results also suggest that the
entire defense response of plants involves a variety of airborne signals in wound-induced volatiles. 相似文献
10.
为研究青藏高原草地承载力的空间演变特征并对其进行预警,以已有的青藏高原净初级生产力数据为基础,核算了该地区的草地理论载畜量及演变趋势,并结合县域实际存栏量,划定了草地承载力的预警等级。结果表明:(1)青藏高原草地承载力整体呈东高西低的格局,其中高寒草原和高寒草甸是该地区草地承载力的主要组成部分;(2)2000-2015年,青藏高原理论载畜量由8614.89万羊单位增至9451.53万羊单位;(3)青藏高原整体处于超载状态,2000-2010年超载状况加剧,至2015年超载状况稍有缓解,草畜平衡指数由67.88%增至79.90%,再降至67.91%。目前亟需优先控制72个红色预警县(超载状态正在加剧)的牲畜存栏量,避免超载状况进一步恶化。未来需要通过控制牲畜存栏量、调整畜牧区发展布局和提高草地生产力等多项措施的结合来改善青藏高原地区的草地承载状况,维持草地生态系统的可持续发展。 相似文献