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Reza Yarani Kamran Mansouri Hamid Reza Mohammadi‐Motlagh Mitra Bakhtiari Ali Mostafaie 《Cell proliferation》2013,46(3):348-355
Objectives
Conventional isolation of epidermis from the dermis and disruption of epidermal sheets to liberate the cells, are performed using proteolytic enzymes such as thermolysin or collagenase. Selective population expansion of melanocytes is achieved by suppressing proliferation of keratinocytes and fibroblasts in epidermal cell suspensions, using phorbol esters and cholera toxin. Here, we introduce a new procedure for isolation of epidermal cells, using proteolytic activity of kiwi fruit actinidin, and also an improved growth medium for melanocytes in the presence of leukaemia inhibitory factor (LIF) and forskolin.Materials and methods
Dermo‐epidermal separation and epidermal sheet cell dispersion were performed using actinidin compared to conventional proteases including collagenase, thermolysin or trypsin. Thereafter, melanocyte culture was performed in two common media and one modified medium to discover optimization for these cells.Results
We found that dermo‐epidermal separation and epidermal sheet cell dispersion using kiwi fruit actinidin were considerably better than previously used methods, both from the aspect of less fibroblast and keratinocyte contamination, and of more viable native cells. Also, melanocytes proliferated better in phorbol ester‐ and cholera toxin‐free proliferation medium supplemented with LIF and forskolin.Conclusion
Less contamination and higher numbers of viable cells were actinidin preferential for separation of epidermis and isolation of epidermal cells. Supplementation of LIF and forskolin to new medium increased proliferation potential of melanocytes in comparison to exogenous mitogens.2.
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Nadezhda T Doncheva Oana Palasca Reza Yarani Thomas Litman Christian Anthon Martien A M Groenen Peter F Stadler Flemming Pociot Lars J Jensen Jan Gorodkin 《Nucleic acids research》2021,49(4):1859
Animal models are crucial for advancing our knowledge about the molecular pathways involved in human diseases. However, it remains unclear to what extent tissue expression of pathways in healthy individuals is conserved between species. In addition, organism-specific information on pathways in animal models is often lacking. Within these limitations, we explore the possibilities that arise from publicly available data for the animal models mouse, rat, and pig. We approximate the animal pathways activity by integrating the human counterparts of curated pathways with tissue expression data from the models. Specifically, we compare whether the animal orthologs of the human genes are expressed in the same tissue. This is complicated by the lower coverage and worse quality of data in rat and pig as compared to mouse. Despite that, from 203 human KEGG pathways and the seven tissues with best experimental coverage, we identify 95 distinct pathways, for which the tissue expression in one animal model agrees better with human than the others. Our systematic pathway-tissue comparison between human and three animal modes points to specific similarities with human and to distinct differences among the animal models, thereby suggesting the most suitable organism for modeling a human pathway or tissue. 相似文献
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Pashaei Somayeh Yarani Reza Mohammadi Pantea Emami Aleagha Mohammad Sajad 《Amino acids》2022,54(6):841-858
Amino Acids - Recently, we reviewed the important role of carbohydrates and lipids metabolism in different clinical aspects of multiple sclerosis (MS) disease. In the current paper, we aimed to... 相似文献
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Asgari Rezvan Yarani Reza Mohammadi Pantea Emami Aleagha Mohammad Sajad 《Cellular and molecular neurobiology》2022,42(7):2121-2129
Cellular and Molecular Neurobiology - Cellular stress can lead to the production of reactive oxygen species (ROS) while autophagy, as a catabolic pathway, protects the cells against stress.... 相似文献
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