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1.
By means of time lapse microcinematography, parameters of growth fluctuations in hydroid polyps Obelia and Dynamena were improved, the pulse pattern of morphogenetic processes was shown, the "antiphase" phenomenon was established in the growth fluctuations of adjacent rudiments of Dynamena. In the phase of rudiment stretching, the length of ectodermal epithelial-muscular cells reduces and their orientation changes, thus leading to the displacement of the whole cellular "column" in the distal direction. In the phase of rudiment partial shortening, endodermal cells loose and lengthen, their internal ends displace in the proximal direction and their external ends slide rapidly in the distal direction with respect to hydrotheca. Changes in the form of rudments arise as a direct mechanical result of periodical contractions of cells densely packed in the epithelial layer. On the basis of these data, morphogenetic processes in hydroid polyps related to the appearance and development of folds and to the rudiment flexures are analyzed. 相似文献
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3.
Identification of alternatively spliced mRNAs encoding potential new regulatory proteins in cattle infected with bovine leukemia virus. 总被引:10,自引:8,他引:2 下载免费PDF全文
S Alexandersen S Carpenter J Christensen T Storgaard B Viuff Y Wannemuehler J Belousov J A Roth 《Journal of virology》1993,67(1):39-52
The polymerase chain reaction was used to detect and characterize low-abundance bovine leukemia virus (BLV) mRNAs. In infected cattle we could detect spliced mRNA with a splice pattern consistent with a Tax/Rex mRNA, as well as at least four alternatively spliced RNAs. Two of the alternatively spliced mRNAs encoded hitherto unrecognized BLV proteins, designated RIII and GIV. The Tax/Rex and alternatively spliced mRNAs could be detected at their highest levels in BLV-infected cell cultures; the next highest levels were found in samples from calves experimentally infected at 6 weeks postinoculation. Alternatively spliced mRNAs were also expressed, albeit at lower levels, in naturally infected animals; they were detected by a nested polymerase chain reaction. Interestingly, the GIV mRNA was specifically detected in naturally infected cows with persistent lymphocytosis and in two of five calves at 6 months after experimental infection with BLV. Furthermore, the calf with the strongest signal for GIV had the highest lymphocyte counts. These data may suggest a correlation between expression of the GIV product and development of persistent lymphocytosis. Some of the donor and acceptor sites in the alternatively spliced mRNAs were highly unusual. The biological mechanisms and significance of such a choice of unexpected splice sites are currently unknown. 相似文献
4.
Submicroscopic rearrangements are described. They serve as the bases of rapid (up to 20 min) changes in the form of the common frog neurula explants: formation of filamentous layer under "naked" surface, appearance of lobopodia on "naked" surface, their "flow", cell polarization and submersion. In all these processes an active part appears to be played by microtubules and microfilaments the bundles of which are always oriented along the long axes of active cells or the directions of passive mechanical tensions. In the cells which are not yet polarized the microtubules form under the surface adjacent to the already polarized cell. This may be considered as one of the chains of cooperative cell polarization. 相似文献
5.
A M Naiakshin E S Belousov A V Taranin S S Bogachev I B Rogozin O K Baranov 《Genetika》1990,26(8):1527-1531
cDNA library in the lambda gt11 phage was constructed using poly (A+)-mRNA from mink spleen as a template. Immunoscreening of the library allowed the identification of 2 lambda-related clones containing 370 and 803 bp insertion (lambda IGL-1 and lambda IGL-2). Analysis of the primary structure of lambda IGL-2 demonstrated that it contains a large portion of V lambda-segment, J lambda-segment, C lambda-gene and its 3'-untranslated part. The nucleotide sequences known for the immunoglobulin genes were compared to the sequence of the lambda IGL-2 clone. The highest degree of homology was established for the rabbit lambda-genes, this being 63, 94 and 72% for the RF3 region of V lambda-segment, J lambda-segment and C lambda-region, respectively. 相似文献
6.
Morozov V. N. Mukhin A. N. Kolyvanova M. A. Belousov A. V. Bushmanov Y. A. Grebennikova T. V. Samoylov A. S. 《Biophysics》2021,66(4):589-595
Biophysics - In recent years, members of the Coronaviridae family have caused outbreaks of respiratory diseases (MERS, SARS, and COVID-19). At the same time, the potential of radiation-induced... 相似文献
7.
Alexei V. Vorobiev Noah K. Scarr Yevgeniy Belousov Eugeny A. Lukhtanov 《Nucleosides, nucleotides & nucleic acids》2013,32(8):421-438
Several 7-(hydroxy, amino, methylureido, and guanidino)alkynyl-substituted 8-aza-7-deaza- hypoxanthine analogues were investigated as potential universal nucleobases. 7-Aminobutynyl-8-aza-7-deazahypoxanthine was found to be the most promising quasi-universal nucleobase with improved hybridization and polymerase chain reaction (PCR) enhancing properties as compared to commonly used hypoxanthine (the nucleobase of inosine). It demonstrated improved ambiguity for pairing with A, T, and C bases and its base pairing properties can be summarized as follows: X:C~X:A~X:T > X:G. The improvement in PCR performance directly correlated with primer's Tm. Primers containing multiple 7-aminobutynyl-8-aza-7-deazahypoxanthines were successfully used without noticeable inhibition of Taq polymerase activity provided the modifications are positioned more than two bases away from the 3′ end. 相似文献
8.
Belousov A. V. Morozov V. N. Krusanov G. A. Moiseev A. N. Davydov A. S. Shtil A. A. Klimanov V. A. Kolyvanova M. A. Samoylov A. S. 《Biophysics》2020,65(4):541-547
Biophysics - Gold nanoparticles are promising radiosensitizers for proton radiotherapy. However, the physical mechanisms of gold nanoparticles radiosensitization remain unclear. In the present... 相似文献
9.
Relaxation of tensions of the surface of Xenopus laevis embryos at the late blastula stage leads to deep and diverse developmental defects and increased variability in mutual position and volume ratios of the axial rudiments. Here, we demonstrate that the development of such embryos was markedly normalized if the relaxed tensions were restored in one of two ways: (1) isotropic stretching of the blastocoel roof induced by incubation of relaxed embryos in a hypotonic medium or (2) anisotropic stretching of embryos on two needles. In the latter case, we succeeded in restoring the morphological axis not only after longitudinal stretching, but also after transverse stretching, and the new axis had signs of anteroposterior polarity. The role of isotropic and anisotropic tensions in organization of the early amphibian development is discussed. 相似文献
10.
Popov EG Kuts FI Belousov OL 《Radiatsionnaia biologiia, radioecologiia / Rossi?skaia akademiia nauk》2002,42(1):86-91
Dose-dependent changes of the molecular characteristics on androgen receptor (AR) systems in gonads of male rats were studied at experimental L-thyroxine-induced states after low doses irradiation exposures in different reference points of 10-km Chernobyl zone. The data obtained suggest a generalized working mechanism of "oscillatorous changes" for contents, affinities and cooperative properties of AR to its natural ligands as a "mirror" reflecting some adaptational reactions in target cells that modulates their androgen-controlled biochemical activity. 相似文献