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The importance of cell cycle analysis in cell culture development has been widely recognised. Whether such analysis is useful
in indicating future performance of high cell density culture is uncertain. Using flow cytometric approach to address this
question, we utilised the fraction of cells in the S phase to control specific growth rate and productivity in spin filter
perfusion cultures and found a significant increase in the accumulated interferon-γ over that obtained from the nutrient-based
controlled fed culture. While a general decrease with time exists in both percentage of S phase cells and specific growth
rate, a clear oscillatory behaviour of both parameters is found in perfusion cultures.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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The relationship between growth and interferon- (IFN-) production in the recombinant cell line CHO 320 was studied by varying the foetal calf serum (FCS) concentration. The specific growth rate varied with the initial FCS concentration in a manner which could be well fitted by the Monod model. TheK
s and
max
values were found to be 0.771% (v/v) serum and 0.031 h–1 respectively. The average specific IFN- production rates during the exponential phase increased with increasing FCS concentration. A good correlation between specific production rate and specific growth rate was found in all phases of the culture except the lag phase and it was clearly demonstrated that IFN- production was growth associated. Specific glucose and glutamine utilisation rates were inversely related to specific growth rates. 相似文献
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Acid hydrolysis of sugarcane bagasse for lactic acid production 总被引:3,自引:0,他引:3
Pattana Laopaiboon Arthit Thani Vichean Leelavatcharamas Lakkana Laopaiboon 《Bioresource technology》2010,101(3):1036-1043
In order to use sugarcane bagasse as a substrate for lactic acid production, optimum conditions for acid hydrolysis of the bagasse were investigated. After lignin extraction, the conditions were varied in terms of hydrochloric (HCl) or sulfuric (H2SO4) concentration (0.5–5%, v/v), reaction time (1–5 h) and incubation temperature (90–120 °C). The maximum catalytic efficiency (E) was 10.85 under the conditions of 0.5% of HCl at 100 °C for 5 h, which the main components (in g l−1) in the hydrolysate were glucose, 1.50; xylose, 22.59; arabinose, 1.29; acetic acid, 0.15 and furfural, 1.19. To increase yield of lactic acid production from the hydrolysate by Lactococcus lactis IO-1, the hydrolysate was detoxified through amberlite and supplemented with 7 g l−1 of xylose and 7 g l−1 of yeast extract. The main products (in g l−1) of the fermentation were lactic acid, 10.85; acetic acid, 7.87; formic acid, 6.04 and ethanol, 5.24. 相似文献
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The role of the cell cycle in determining gene expression and productivity in CHO cells 总被引:3,自引:0,他引:3
Understanding the relationships between cell cycle and protein expression is critical to the optimisation of media and environmental
conditions for successful commercial operation of animal cell culture processes. Using flow cytometry for the analysis of
the early phases of synchronised batch cultures, the dependency of product expression on cell cycle related events has been
evaluated in a recombinant CHO cell line. Although the production of recombinant protein is initially found to be cell cycle
related, the maximum specific protein productivity is only achieved at a later stage of the exponential phase which also sees
a maximum in the intracellular protein concentration. Subsequent work suggests that it is the batch phase/medium composition
of cultures which is the major determinant of maximum specific productivity in this cell line. Furthermore the effect of the
positive association between S phase and specific productivity is subordinate to the effect of batch phase/medium composition
on the specific productivity of batch cultures.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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