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1.
The microbial community in the soil was analyzed during four weeks of a continuous enrichment of structural chernozem soil samples with a 0.1% solution of carboxymethyl cellulose (CMC) under aerobic and semianaerobic conditions. During the first 14 d, the total amount of the aerobic and anaerobic, cellulose-degrading microorganisms increased significantly. Various metabolic pathways were u‘ed te decompose the substrate: diverse metabolic systems were activated and different groups of microorganisms preferred in dependence on the presence of oxygen or the source of mineral nitrogen. In the later phases of cultivation, a decrease in the concentration of zymogenous microflora and in the level of substrate mineralization was observed ovon though CM-cellulase activity remained high. During the fourth week of cultivation, a conspicuous increase in the numbers of oligothropic bacteria occurring in the colcnies of the microorganisms degrading cellulose was found. The representatives of prosthecobacteria (Caulobacter, Hyphomicrobium, Prosthecomicrobium spp.) andSeliberia sp. were thus identified. This “microflora of dispersion” attends the zymogenous microbes degrading CMC and indicates later phases of the process of decomposition. 相似文献
2.
Saks V. A. Khuchua Z. A. Vasilyeva E. V. Belikova O. Yu Kuznetsov A. V. 《Molecular and cellular biochemistry》1994,133(1):155-192
The published experimental data and existing concepts of cellular regulation of respiration are analyzed. Conventional, simplified considerations of regulatory mechanism by cytoplasmic ADP according to Michaelis-Menten kinetics or by derived parameters such as phosphate potential etc. do not explain relationships between oxygen consumption, workload and metabolic state of the cell. On the other hand, there are abundant data in literature showing microheterogeneity of cytoplasmic space in muscle cells, in particular with respect to ATP (and ADP) due to the structural organization of cell interior, existence of multienzyme complexes and structured water phase. Also very recent experimental data show that the intracellular diffusion of ADP is retarded in cardiomyocytes because of very low permeability of the mitochondrial outer membrane for adenine nucleotidesin vivo. Most probably, permeability of the outer mitochondrial membrane porin channels is controlled in the cellsin vivo by some intracellular factors which may be connected to cytoskeleton and lost during mitochondrial isolation. All these numerous data show convincingly that cellular metabolism cannot be understood if cell interior is considered as homogenous solution, and it is necessary to use the theories of organized metabolic systems and substrate-product channelling in multienzyme systems to understand metabolic regulation of respiration. One of these systems is the creatine kinase system, which channels high energy phosphates from mitochondria to sites of energy utilization. It is proposed that in muscle cells feed-back signal between contraction and mitochondrial respiration may be conducted by metabolic wave (propagation of oscillations of local concentration of ADP and creatine) through cytoplasmic equilibrium creatine and adenylate kinases and is amplified by coupled creatine kinase reaction in mitochondria. Mitochondrial creatine kinase has experimentally been shown to be a powerful amplifier of regulatory action of weak ADP fluxes due to its coupling to adenine nucleotide translocase. This phenomenon is also carefully analyzed.It is easier to explain biochemistry in terms of transport than it is to explain transport in terms of biochemistry. P. Mitchell The Ninth Sir Hans Krebs Lecture, Dresden, July 2, 1978. 相似文献
3.
Kuznetsova S. A. Vasilyeva N. Yu. Drozd N. N. Mikhailenko M. A. Shakhtshneider T. P. Malyar Yu. N. Kuznetsov B. N. Chesnokov N. V. 《Russian Journal of Bioorganic Chemistry》2020,46(7):1323-1329
Russian Journal of Bioorganic Chemistry - A comparison of IR spectra and molecular weight distribution of arabinogalactan sulfates as sodium and ammonium salts obtained using various sulfating... 相似文献
4.
Vasilyeva A. D. Ivanov V. S. Yurina L. V. Indeykina M. I. Bugrova A. E. Kononikhin A. S. Nikolaev E. N. Rosenfeld M. A. 《Doklady. Biochemistry and biophysics》2021,501(1):419-423
Doklady Biochemistry and Biophysics - Plasminogen is a zymogenic form of plasmin, an enzyme that plays a fundamental role in the dissolution of fibrin clots as well as in many other physiological... 相似文献
5.
Hakobyan A. V. Burakova E. A. Arabyan E. A. Fokina A. A. Kotsinyan A. R. Vasilyeva S. V. Zakaryan O. S. Stetsenko D. A. 《Russian Journal of Bioorganic Chemistry》2021,47(2):411-419
Russian Journal of Bioorganic Chemistry - Antiviral activity of antisense oligodeoxyribonucleotides with phosphorothioate or mesyl phosphoramidate internucleotidic groups targeting the main capsid... 相似文献
6.
Larissa N. Vasilyeva Aleksey V. Chernyshev Steven L. Stevenson Kevin D. Hyde 《Mycoscience》2013,54(2):110-115
Two new species collected in northern Thailand are herein described and discussed. Tortulomyces thailandicus is the type of a new genus characterized by a combination of such features as a dense subiculum, non-ostiolate ascomata, two kinds of paraphyses (filiform and bead-like), and 1-septate, brown ascospores. Nitschkia siamensis has non-ostiolate, turbinate and stalked ascomata and hyaline, often slightly curved, 1-septate ascospores. 相似文献
7.
Zakhar O. Shenkarev Mikhail A. Shulepko Maxim L. Bychkov Dmitrii S. Kulbatskii Olga V. Shlepova Nathalia A. Vasilyeva Alexander A. Andreev-Andrievskiy Anfisa S. Popova Evgeniya A. Lagereva Eugene V. Loktyushov Sergey G. Koshelev Morten S. Thomsen Dmitry A. Dolgikh Sergey A. Kozlov Pavel M. Balaban Mikhail P. Kirpichnikov Ekaterina N. Lyukmanova 《Journal of neurochemistry》2020,155(1):45-61
8.
O. B. Vasilyeva M. A. Nazarova P. O. Ripatti N. N. Nemova 《Contemporary Problems of Ecology》2016,9(2):177-187
We studied the growth performance and lipid composition of liver, muscles and visceral fat in rainbow trout Parasalmo mykiss (Walbaum, 1792) and the diets used in its cultivation during the spring-summer season. The differences in dietary lipid composition determined modifications in the biochemical composition of tissues of rainbow trout and had an effect on the weight and growth rate of the fish. Trophic and seasonal factors also had their influence on the studied parameters. The degree of influence depended on the functional characteristics of tissues and organs of rainbow trout. 相似文献
9.
E. V. Borvinskaya I. V. Sukhovskaya A. A. Kochneva O. B. Vasilyeva M. A. Nazarova L. P. Smirnov N. N. Nemova 《Contemporary Problems of Ecology》2016,9(2):195-202
The base levels and seasonal variability of ethoxyresorufin-O-deethylase (EROD) activity, glutathione-S-transferase (GST) activity, reduced glutathione concentration (GSH) and phospholipids concentration (PhL) of Coregonus lavaretus (Linnaeus, 1758) and Coregonus muksun (Pallas, 1814) were investigated. The relationships between this biochemical markers, hepatosomatic index and seasonal growth were also examined. 相似文献
10.
Vasilyeva A. E. Yanshina D. D. Karpova G. G. Malygin A. A. 《Russian Journal of Bioorganic Chemistry》2019,45(6):758-765
Russian Journal of Bioorganic Chemistry - The ribosomal protein uS15 is one of the key proteins forming the small (40S) ribosomal subunit structure around the central domain of 18S rRNA. According... 相似文献