全文获取类型
收费全文 | 347篇 |
免费 | 34篇 |
出版年
2021年 | 3篇 |
2020年 | 3篇 |
2019年 | 1篇 |
2018年 | 6篇 |
2017年 | 10篇 |
2016年 | 9篇 |
2015年 | 13篇 |
2014年 | 20篇 |
2013年 | 25篇 |
2012年 | 28篇 |
2011年 | 20篇 |
2010年 | 22篇 |
2009年 | 13篇 |
2008年 | 17篇 |
2007年 | 13篇 |
2006年 | 23篇 |
2005年 | 16篇 |
2004年 | 24篇 |
2003年 | 18篇 |
2002年 | 18篇 |
2001年 | 9篇 |
2000年 | 9篇 |
1999年 | 4篇 |
1998年 | 4篇 |
1997年 | 6篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 5篇 |
1991年 | 13篇 |
1990年 | 5篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1982年 | 2篇 |
1978年 | 1篇 |
排序方式: 共有381条查询结果,搜索用时 24 毫秒
1.
2.
3.
4.
Dissection of the bifunctional ARGRII protein involved in the regulation of arginine anabolic and catabolic pathways. 总被引:11,自引:5,他引:6 下载免费PDF全文
ARGRII is a regulatory protein which regulates the arginine anabolic and catabolic pathways in combination with ARGRI and ARGRIII. We have investigated, by deletion analysis and fusion to LexA protein, the different domains of ARGRII protein. In contrast to other yeast regulatory proteins, 92% of ARGRII is necessary for its anabolic repression function and 80% is necessary for its catabolic activator function. We can define three domains in this protein: a putative DNA-binding domain containing a zinc finger motif, a region more involved in the repression activity located around the RNase-like sequence, and a large activation domain. 相似文献
5.
6.
7.
Summary A membrane interactive peptide was toxic to microspores, pollen and protoplasts of canola in the 1–5 µM concentration range. Similarly, at 5.0 µM the peptide completely inhibited germination of conidia ofVerticillium albo-atrum; however, when tested with conidia of a virulent isolate of blackleg (Leptosphaeria maculens), a fungal pathogen of canola, much higher levels (>30 µM) of the peptide were required to reduce or arrest germination and growth of the conidia. When testing the relative toxicities of novel peptides on plant cells and their pathogens, pollen germination is a simple, rapid and reliable alternative to protoplasts. 相似文献
8.
以含有水稻条纹叶枯病毒(RSV)外壳蛋白(CP)基因的重组克隆为材料,将RSV-cDNA酶解后,亚克隆人M13mp18、19,采用双脱氧链终止法测序得到RSV-CP基因的全长序列(共含966bp),同日本已发表的RSV-CP基因序列相比同源率达97%。 相似文献
9.
Herbert M. Geller Vanya Qui;ones-Jenab Maciej Poltorak William J. Freed 《Journal of cellular biochemistry》1991,45(3):279-283
Immortalized cell lines can serve as model systems for studies of neuronal development and restoration of function in models of neurological disease. Cell lines which result from spontaneous or experimentally-induced tumors have been used for these purposes. More recently, the techniques of genetic engineering have resulted in the production of cell lines with specific desired characteristics. This has been accomplished by insertion of a desired gene into a pre-existing immortal cell or by immortalizing primary cells. The production of immortal cell lines using temperature-sensitive immortalizing genes offers an additional method of controlling gene expression, and thereby controlling cell proliferation and differentiation. In the nervous system, these techniques have produced immortal cell lines with neuronal and glial properties. 相似文献
10.
Vimentin and 70K Neurofilament Protein Co-exist in Embryonic Neurones from Spinal Ganglia 总被引:7,自引:3,他引:4
Abstract: The mesenchymal intermediate filament protein vimentin and the 70K component of neurofilament were detected by two-dimensional gel electrophoresis in cultures of pure sensory and sympathetic neurones derived from chick embryos. The identities of these neuronal intermediate filament proteins were confirmed by comparison of their molecular weights, isoelectric points, and peptide patterns from limited papain digestions with those of the corresponding proteins from fibroblasts and brain, respectively. A specific antibody to vimentin stained filamenteous structures and colcemid-induced coils in both neurones and associated satellite cells. In contrast, a specific antibody to the 70K neurofilament protein stained these structures solely in neurones. This neurone-specific staining, as well as its molecular weight and isoelectric point, distinguishes the 70K neurofilament protein from the 68K neurofilament as sociated protein described by others, which has been claimed to resemble the tubulin assembly protein. 相似文献