排序方式: 共有92条查询结果,搜索用时 15 毫秒
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G Vandenbussche A Clercx M Clercx T Curstedt J Johansson H J?rnvall J M Ruysschaert 《Biochemistry》1992,31(38):9169-9176
Attenuated total reflection Fourier transform infrared spectroscopy was used to investigate the secondary structure of the surfactant protein SP-B. Nearly half of the polypeptide chain is folded in an alpha-helical conformation. No significant change of the secondary structure content was observed when the protein is associated to a lipid bilayer of dipalmitoylphosphatidylcholine (DPPC)/phosphatidylglycerol (PG) or of dipalmitoylphosphatidylglycerol (DPPG). The parameters related to the gamma w(CH2) vibration of the saturated acyl chains reveal no modification of the conformation or orientation of the lipids in the presence of SP-B. A model of orientation of the protein at the lipid/water interface is proposed. In this model, electrostatic interactions between charged residues of SP-B and polar headgroups of PG, and the presence of small hydrophobic alpha-helical peptide stretches slightly inside the bilayers, would maintain SP-B at the membrane surface. 相似文献
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J. P. Fryns M. Haspeslagh E. Vandenbussche P. Goddeeris E. Eggermont H. Van den Berghe 《Human genetics》1980,56(2):225-226
Summary An XX/XY mosaicism was detected in two phenotypically male newborns with perinatal death. The importance of chromosome studies at the paediatric necropsy and the significance of the present findings are discussed. 相似文献
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The antiviral activity of the type-2 ribosome-inactivating protein (RIP) IRAb from Iris was analyzed by expressing IRAb in tobacco (Nicotiana tabacum L. cv. Samsun NN) plants and challenging the transgenic plants with tobacco mosaic virus (TMV). Although constitutive expression of IRAb resulted in an aberrant phenotype, the plants were fertile. Transgenic tobacco lines expressing IRAb showed a dose-dependent enhanced resistance against TMV infection but the level of protection was markedly lower than in plants expressing IRIP, the type-1 RIP from Iris that closely resembles the A-chain of IRAb. To verify whether IRIP or IRAb can also confer systemic protection against viruses, transgenic RIP-expressing scions were grafted onto control rootstocks and leaves of the rootstocks challenged with tobacco etch virus (TEV). In spite of the strong local antiviral effect of IRIP and IRAb the RIPs could not provide systemic protection against TEV. Hence our results demonstrate that expression of the type-1 and type-2 RIPs from Iris confers tobacco plants local protection against two unrelated viruses. The antiviral activity of both RIPs was not accompanied by an induction of pathogenesis-related proteins. It is suggested that the observed antiviral activity of both Iris RIPs relies on their RNA N-glycohydrolase activity towards TMV RNA and plant rRNA.Abbreviations GUS -Glucuronidase - IRAb Iris agglutinin b - IRIP Iris type-1 RIP - PAG Polynucleotide:adenosine glycosylase - PAP Phytolacca americana antiviral protein - PR Pathogenesis-related - RIP Ribosome-inactivating protein - TCS Trichosanthin - TEV Tobacco etch virus - TMV Tobacco mosaic virus 相似文献
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Shaping the shoot: a circuitry that integrates multiple signals 总被引:1,自引:0,他引:1
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Desmyter S Vandenbussche F Van Damme EJ Peumans WJ 《Preparative biochemistry & biotechnology》2001,31(3):209-216
A simple, but highly efficient, method was developed for the purification of monospecific antibodies against the plant glycoprotein Sambucus nigra lectin related protein. In a first step, the antiserum is purified by affinity chromatography on a column with the immobilized antigen. To deplete the affinity-purified antiserum from aspecific cross-reacting antibodies directed against the glycan part of the glycoprotein, a second affinity chromatography on an unrelated plant glycoprotein, in casu the Robinia pseudoacacia agglutinin, is included. 相似文献
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Role of the carboxy terminus of Escherichia coli FtsA in self-interaction and cell division 下载免费PDF全文
Yim L Vandenbussche G Mingorance J Rueda S Casanova M Ruysschaert JM Vicente M 《Journal of bacteriology》2000,182(22):6366-6373
The role of the carboxy terminus of the Escherichia coli cell division protein FtsA in bacterial division has been studied by making a series of short sequential deletions spanning from residue 394 to 420. Deletions as short as 5 residues destroy the biological function of the protein. Residue W415 is essential for the localization of the protein into septal rings. Overexpression of the ftsA alleles harboring these deletions caused a coiled cell phenotype previously described for another carboxy-terminal mutation (Gayda et al., J. Bacteriol. 174:5362-5370, 1992), suggesting that an interaction of FtsA with itself might play a role in its function. The existence of such an interaction was demonstrated using the yeast two-hybrid system and a protein overlay assay. Even these short deletions are sufficient for impairing the interaction of the truncated FtsA forms with the wild-type protein in the yeast two-hybrid system. The existence of additional interactions between FtsA molecules, involving other domains, can be postulated from the interaction properties shown by the FtsA deletion mutant forms, because although unable to interact with the wild-type and with FtsADelta1, they can interact with themselves and cross-interact with each other. The secondary structures of an extensive deletion, FtsADelta27, and the wild-type protein are indistinguishable when analyzed by Fourier transform infrared spectroscopy, and moreover, FtsADelta27 retains the ability to bind ATP. These results indicate that deletion of the carboxy-terminal 27 residues does not alter substantially the structure of the protein and suggest that the loss of biological function of the carboxy-terminal deletion mutants might be related to the modification of their interacting properties. 相似文献
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Filip Vandenbussche Dominique Van Der Straeten 《Journal of Plant Growth Regulation》2007,26(2):178-187
The plant hormone ethylene plays a pivotal role in steering various processes by regulating the biosynthesis, distribution,
or signal transduction of other hormones. Ethylene also mediates the effects of other hormones. Similarly, hormones control
the ethylene synthesis and signalling pathway. Eventually, integration of this network of signals leads to an appropriate
morphological or biochemical response. Consequently, this cross-talk results in the characteristic plasticity associated with
plant development. Here, the interplay of ethylene with other hormones is described for germination and seedling growth, stomatal
control, and tissue elongation. The mechanisms by which this occurs are discussed in more detail. 相似文献
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Filip Vandenbussche Ana C Fierro Gertrud Wiedemann Ralf Reski Dominique Van Der Straeten 《BMC plant biology》2007,7(1):65