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Immunocytochemical Localization of Glutamine Synthetase in Organs of Phaseolus vulgaris L 总被引:4,自引:2,他引:2 下载免费PDF全文
Glutamine synthetase was localized in nodules, roots, stems, and leaves of red kidney bean (Phaseolus vulgaris L.) by immunocytochemistry. Affinity purified antibodies reactive with glutamine synthetase were prepared using purified nodule-enhanced glutamine synthetase. Immunogold labeling was observed in the cell cytoplasm in each plant organ. In nodules, the labeling was more intense in the infected cells than in the uninfected cells. No labeling was observed in nodule bacteroids, peribacteroid spaces, or in peribacteroid membranes, while previous reports of glutamine synthetase immunolabeling of legume nodules showed labeling in the bacteroid fraction. Significant labeling was observed in nodule proplastids which contained starch granules. Substantial labeling was also observed in leaf chloroplasts. No labeling was observed in other organelles including mitochondria, peroxisomes, and endoplasmic reticulum. Preimmune IgGs did not bind to any structure in the tissues examined. 相似文献
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The apoplastic fluids of field-grown Zea mays and Zea luxurians plants were isolated from surface sterilized stem tissue by centrifugation and spread on agar plates containing a nitrogen-free, defined medium. The predominant bacterium isolated from these plates was characterized further. The ability of this bacterium to fix nitrogen was confirmed by its ability to grow on a semi-solid, nitrogen-free medium and reduce 15N2 to 15NH3 and acetylene to ethylene. Protions of the nifH and 16S rRNA genes from this organism were amplified by PCR and sequenced. The nifH gene, which codes for dinitrogenase reductase, from this organism is closely related to nifH from Klebsiella pneumoniae. Similarly, the 16S rRNA gene sequences and carbon utilization tests grouped it closely with K. pneumoniae. Based an these data, the isolates from Z. mays and Z. luxurians are tentatively classified as Klebsiella spp. (Zea). The ability of this bacterium to contribute to the nitrogen economy of the corn plant is unknown. 相似文献
4.
Eric W. Triplett Dale G. Blevins Douglas D. Randall 《Archives of biochemistry and biophysics》1982,219(1):39-46
Xanthine dehydrogenase (EC 1.2.1.37), an essential enzyme for ureide metabolism was purified from the cytosol fraction of soybean nodules. The purified xanthine dehydrogenase was shown to be homogeneous by electrophoresis and a pI of 4.7 was determined by isoelectric focusing. The enzyme had a molecular weight of 285,000 and two subunits of molecular weight 141,000 each. The holoenzyme contained 1.7 (±0.7) mol Mo and 8.1 (±2.0) mol Fe/mol enzyme and the enzyme also contained FMN and is thus a molybdoironflavoprotein. Soybean xanthine dehydrogenase is the second enzyme in plants demonstrated to contain Mo and the first xanthine-oxidizing enzyme reported to contain FMN, rather than FAD as the flavin cofactor. 相似文献
5.
Allantoic Acid Synthesis in Soybean Root Nodule Cytosol via Xanthine Dehydrogenase 总被引:21,自引:13,他引:8 下载免费PDF全文
Allantoin and allantoic acid are the major forms of nitrogen transported from soybean nodules to other parts of the plant. Neither the pathway or the site of ureide synthesis has been demonstrated in root nodules. 相似文献
6.
Ross B. Mikkelsen David H. Tang Edward L. Triplett 《Biochemical and biophysical research communications》1975,63(4):980-985
Purified tyrosinase from is activated by light. An action spectrum for the process indicates that there are two absorption bands responsible for the activation (290nm and 334nm). The kinetics of the photochemical process show an initial activation followed by inhibition. Molecular oxygen is required. The ability of the protein to be photoactivated and the absorbancy of the protein at 334nm can be extracted with 50% acetone/water. 相似文献
7.
Pseudosasa xishuangbannaensis is described and illustrated as a new species of Arundinarieae (the temperate woody bamboos) from southern Yunnan, China. It belongs to Pseudosasa subg. Sinicae. The new species is characterized by glabrous culm leaf sheaths with dense setae at the base, several to many spikelets, and 5–7 glumes, and it can be distinguished from closely related species by these morphological features and molecular evidence. 相似文献
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Dong Y Glasner JD Blattner FR Triplett EW 《Applied and environmental microbiology》2001,67(4):1911-1921