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Membrane-trafficking RabA4c involved in the effect of glycine betaine on recovery from chilling stress in Arabidopsis 总被引:2,自引:0,他引:2
John Einset Erik Nielsen Erin L. Connolly Atle Bones Torfinn Sparstad Per Winge Jian-Kang Zhu 《Physiologia plantarum》2007,130(4):511-518
Glycine betaine (GB) can confer tolerance to several types of stress at low concentrations, either after application to plants or in transgenics engineered to overproduce GB. Based on earlier studies on levels of GB in plants and evidence for effects on gene expression, we hypothesized that at least part of this effect could be ascribed to the activation of the expression of stress tolerance genes. Using a strategy based on high-throughput gene expression analysis with microarrays followed by confirmation with northern blots, we identified Arabidopsis genes upregulated in roots that reinforce intracellular processes protecting cells from oxidative damage and others that appear to be involved in reinforcing a scavenging system for reactive oxygen species (ROS) in cell walls. Upregulated genes in roots include those for the membrane-trafficking RabA4c, the root-specific NADPH-dependent ferric reductase (FRO2) localized to the plasma membrane, mitochondrial catalase 2 and the cell wall peroxidase ATP3a. Comparative studies with wild-type Arabidopsis and knockout mutants for the membrane-trafficking RabA4c gene demonstrated that the mutants respond only slightly to GB, if at all, compared with wild-type in relation to root growth recovery after chilling stress, demonstrating the role of RabA4c in relation to the GB effect. The results point toward links between oxidative stress, gene expression, membrane trafficking and scavenging of ROS such as superoxide and hydrogen peroxide in relation to GB effects on chilling tolerance in plants. 相似文献
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The pelagic fish stock (mostly lavnun, Mirogrex terraesanctae) of Lake Kinneret was assessed using HADAS, a newly developed echo-counting analysis system. Total fish numbers (during Mar–Apr) were estimated to be 61 million in 1987, 218 million in 1988, 120 million in 1989, and 69 million in April 1990. The total fish number for March 1981 was recalculated from published data and estimated at 170 million. The decline in total numbers between 1988 and 1990 resulted primarily from a decrease in the relative abundance of the smallest size classes of fish. Since fish abundance apparently declined between 1981 and 1990, the decline in zooplankton standing stocks documented during these years cannot be attributed to higher fish predation caused by larger fish stocks. The HADAS estimates of lavnun population size are large enough to account for the known catch of lavnun.Contribution from Israel Oceanographic & Limnological Research, Ltd. This research was supported in part by the Israeli Water Commissioner, the Israeli Electric Company, and by a grant from the U.S. Agency for International Development. 相似文献
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Exitianus obscurinervis (Hemiptera: Cicadellidae), a new experimental vector of Spiroplasma kunkelii
Carloni E Virla E Paradell S Carpane P Nome C Laguna I Giménez Pecci MP 《Journal of economic entomology》2011,104(6):1793-1799
"Corn stunt" caused by the mollicute Spiroplasma kunkelii (Whitcomb) is potentially one of the most severe diseases affecting the corn (Zea mays L.) crop in the Americas, and the leafhopper Dalbulus maidis (DeLong & Wolcott) is considered its most important vector. However, other insects seen quite frequently in corn crops might well be its vectors in Argentina To identify any leafhoppers species other than D. maidis that can transmit S. kunkelii, transmission assays were conducted, using individuals of Exitianus obscurinervis (St?l) collected in field and reared under controlled conditions. S. kunkelii was transmitted to corn plants by E. obscurinervis. The pathogen was transmitted to seven of the 11 plants, which showed characteristic corn stunt symptoms, and the presence of the pathogen was confirmed by DAS-ELISA. The presence of S. kunkelii in the E. obscurinervis individuals used in transmission experiments was confirmed by polymerase chain reaction and electron microscopy. The current study shows the existence of a new experimental vector of S. kunkelii, the leafhopper E. obscurinervis, which acquired spiroplasmas from infected plants and inoculated it to healthy plants. 相似文献
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Two adjacent inversions maintain genomic differentiation between migratory and stationary ecotypes of Atlantic cod 下载免费PDF全文
Tina Graceline Kirubakaran Harald Grove Matthew P. Kent Simen R. Sandve Matthew Baranski Torfinn Nome Maria Cristina De Rosa Benedetta Righino Torild Johansen Håkon Otterå Anna Sonesson Sigbjørn Lien Øivind Andersen 《Molecular ecology》2016,25(10):2130-2143
Atlantic cod is composed of multiple migratory and stationary populations widely distributed in the North Atlantic Ocean. The Northeast Arctic cod (NEAC) population in the Barents Sea undertakes annual spawning migrations to the northern Norwegian coast. Although spawning occurs sympatrically with the stationary Norwegian coastal cod (NCC), phenotypic and genetic differences between NEAC and NCC are maintained. In this study, we resolve the enigma by revealing the mechanisms underlying these differences. Extended linkage disequilibrium (LD) and population divergence were demonstrated in a 17.4‐Mb region on linkage group 1 (LG1) based on genotypes of 494 SNPs from 192 parents of farmed families of NEAC, NCC or NEACxNCC crosses. Linkage analyses revealed two adjacent inversions within this region that repress meiotic recombination in NEACxNCC crosses. We identified a NEAC‐specific haplotype consisting of 186 SNPs that was fixed in NEAC sampled from the Barents Sea, but segregating under Hardy–Weinberg equilibrium in eight NCC stocks. Comparative genomic analyses determine the NEAC configuration of the inversions to be the derived state and date it to ~1.6–2.0 Mya. The haplotype block harbours 763 genes, including candidates regulating swim bladder pressure, haem synthesis and skeletal muscle organization conferring adaptation to long‐distance migrations and vertical movements down to large depths. Our results suggest that the migratory ecotype experiences strong directional selection for the two adjacent inversions on LG1. Despite interbreeding between NEAC and NCC, the inversions are maintaining genetic differentiation, and we hypothesize the co‐occurrence of multiple adaptive alleles forming a ‘supergene’ in the NEAC population. 相似文献
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Odd Terje Sandlund Hilmar J. Malmquist Bror Jonsson Skúli Skúlason Sigurdur S. Snorrason Pétur M. Jónasson Rolf Gydemo Torfinn Lindem 《Environmental Biology of Fishes》1988,23(3):183-195
Synopsis Population densities of age-0 arctic chaff in the surf zone averaged 1.83 and 4.70 fish m-2 in August 1984 and June 1985, respectively. Length variation of the littoral fish was low in early summer, increasing in
late summer and autumn. Newly emerged charr, ∼ 20 mm long, appeared in the stony shallow water zone during both May and June.
From length variation and variation in mouth position of the young charr, it is concluded that at least two of the four chaff
morphs in the lake are present in the surf zone during spring and summer. In August, some of the larger age-0 charr had moved
out from the surf zone, into the pelagic and the deeper epibenthic waters. The food of young littoral charr was dominated
by large chironomid larvae (instar 3 and 4) and pupae
Contribution from the Thingvallavatn project 相似文献
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Dystocia and stillbirth are significant causes of female and neonatal death in many species and there is evidence for a genetic component to both traits. Identifying causal mutations affecting these traits through genome wide association studies could reveal the genetic pathways involved and will be a step towards targeted interventions. Norwegian Red cattle are an ideal model breed for such studies as very large numbers of records are available. We conducted a genome wide association study for direct and maternal effects of dystocia and stillbirth using almost 1 million records of these traits. Genotyping costs were minimized by genotyping the sires of the recorded cows, and using daughter averages as phenotypes. A dense marker map containing 17 343 single nucleotide polymorphisms covering all autosomal chromosomes was utilized. The genotyped sires were assigned to one of two groups in an attempt to ensure independence between the groups. Associations were only considered validated if they occurred in both groups. Strong associations were found and validated on chromosomes 4, 5, 6, 9, 12, 20, 22 and 28. The QTL region on chromosome 6 was refined using LDLA analysis. The results showed that this chromosome most probably contains two QTL for direct effect on dystocia and one for direct effect on stillbirth. Several candidate genes may be identified close to these QTL. Of these, a cluster of genes expected to affect bone and cartilage formation (i.e. SPP1, IBSP and MEPE) are of particular interest and we suggest that these genes are screened in candidate gene studies for dystocia and stillbirth in cattle as well as other species. 相似文献
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H. Nilsen H. G. Olsen B. Hayes T. Nome E. Sehested M. Svendsen T. H. E. Meuwissen S. Lien 《Animal genetics》2009,40(5):701-712
Quantitative trait loci affecting clinical mastitis were detected and fine mapped to a narrow region on bovine chromosome 6 in the Norwegian Red cattle population. The region includes the casein gene cluster and several candidate genes thought to influence clinical mastitis. The most significant results were found for SNPs within the Mucin 7 gene. This gene encodes an antimicrobial peptide and constitutes part of the first line of defence for the mucosal immune system. Detection of long haplotypes extending several Mb may indicate that artificial selection has influenced the haplotype structures in the region. A search for selection sweeps supports this observation and coincides with association results found both by single SNP and haplotype analyses. Our analyses identified haplotypes carrying quantitative trait loci alleles associated with high protein yield and simultaneously fewer incidences of clinical mastitis. The fact that such haplotypes are found in relative high frequencies in Norwegian Red may reflect the combined breeding goal that is characterized by selection for both milk production and disease resistance. The identification of these haplotypes raises the possibility of overcoming the unfavourable genetic correlation between these traits through haplotype-assisted selection. 相似文献
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Torfinn Nome Gard O.S. Thomassen Jarle Bruun Terje Ahlquist Anne C. Bakken Andreas M. Hoff Torleiv Rognum Arild Nesbakken Susanne Lorenz Jinchang Sun João Diogo Barros-Silva Guro E. Lind Ola Myklebost Manuel R. Teixeira Leonardo A. Meza-Zepeda Ragnhild A. Lothe Rolf I. Skotheim 《Translational oncology》2013,6(5):546-IN5