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Vacuolar H+-ATPase is involved in preventing heavy metal-induced oxidative stress in Saccharomyces cerevisiae 总被引:1,自引:0,他引:1
Todsapol Techo Supat Jindarungrueng Supinda Tatip Tossapol Limcharoensuk Prayad Pokethitiyook Maleeya Kruatrachue Choowong Auesukaree 《Environmental microbiology》2020,22(6):2403-2418
In Saccharomyces cerevisiae, vacuolar H+-ATPase (V-ATPase) involved in the regulation of intracellular pH homeostasis has been shown to be important for tolerances to cadmium, cobalt and nickel. However, the molecular mechanism underlying the protective role of V-ATPase against these metals remains unclear. In this study, we show that cadmium, cobalt and nickel disturbed intracellular pH balance by triggering cytosolic acidification and vacuolar alkalinization, likely via their membrane permeabilizing effects. Since V-ATPase plays a crucial role in pumping excessive cytosolic protons into the vacuole, the metal-sensitive phenotypes of the Δvma2 and Δvma3 mutants lacking V-ATPase activity were supposed to result from highly acidified cytosol. However, we found that the metal-sensitive phenotypes of these mutants were caused by increased production of reactive oxygen species, likely as a result of decreased expression and activities of manganese superoxide dismutase and catalase. In addition, the loss of V-ATPase function led to aberrant vacuolar morphology and defective endocytic trafficking. Furthermore, the sensitivities of the Δvma mutants to other chemical compounds (i.e. acetic acid, H2O2, menadione, tunicamycin and cycloheximide) were a consequence of increased endogenous oxidative stress. These findings, therefore, suggest the important role of V-ATPase in preventing endogenous oxidative stress induced by metals and other chemical compounds. 相似文献
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Techo Sujitra Visessanguan Wonnop Vilaichone Ratha-korn Tanasupawat Somboon 《Probiotics and antimicrobial proteins》2019,11(1):92-102
Probiotics and Antimicrobial Proteins - A total of 32 lactic acid bacteria (LAB) were isolated from Khanom-jeen, a Thai traditional fermented rice noodle. They belonged to the genus Leuconostoc... 相似文献
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