全文获取类型
收费全文 | 256篇 |
免费 | 3篇 |
出版年
2022年 | 4篇 |
2021年 | 7篇 |
2020年 | 8篇 |
2019年 | 3篇 |
2018年 | 8篇 |
2017年 | 10篇 |
2016年 | 5篇 |
2014年 | 6篇 |
2013年 | 11篇 |
2012年 | 14篇 |
2011年 | 10篇 |
2010年 | 8篇 |
2009年 | 10篇 |
2008年 | 14篇 |
2007年 | 13篇 |
2006年 | 12篇 |
2005年 | 9篇 |
2004年 | 11篇 |
2003年 | 8篇 |
2002年 | 11篇 |
2001年 | 4篇 |
2000年 | 4篇 |
1999年 | 2篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1987年 | 4篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1983年 | 3篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1977年 | 3篇 |
1976年 | 4篇 |
1975年 | 7篇 |
1974年 | 3篇 |
1973年 | 5篇 |
1972年 | 3篇 |
1971年 | 2篇 |
1970年 | 2篇 |
1969年 | 2篇 |
1968年 | 1篇 |
1967年 | 2篇 |
1965年 | 2篇 |
排序方式: 共有259条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
Lioubov I. Tikhonova Igor I. Pottosin Karl-Josef Dietz Gerald Schönknecht 《The Plant journal : for cell and molecular biology》1997,11(5):1059-1070
In contrast to the vacuolar ion channels which are gated open by an increase of cytosolic Ca2+ the vacuolar ion currents at resting cytosolic Ca2+ are poorly explored. Therefore, this study was performed to investigate the properties of the so-called fast-activating vacuolar (FV) current which dominates the electrical characteristics of the tonoplast at physiological free Ca2+ concentrations. Patch—clamp measurements were performed on whole barley ( Hordeum vulgare ) mesophyll vacuoles and on excised tonoplast patches. Single ion channels were identified, which, based on their selectivity, activation kinetics, Ca2+ - and voltage-dependence, carry the whole-vacuole FV current. Reversal potential determinations indicated a K+ overs C− permeability ratio of about 30. Both inward and outward whole-vacuole currents as well as the activity of single FV channels were inhibited by an increase of cytosolic Ca2+ , with a Kd ≈ 6 µM. At physiological vacuolar Ca2+ activities, the FV channel is an outward-rectifying potassium channel. The FV channel was activated in less than a few milliseconds both by negative and positive potential steps, having a minimal activity that is 40 mV negative of the K+ equilibrium potential. It is proposed that transport of K+ through this cation channel controls the electrical potential difference across the tonoplast. 相似文献
5.
A biological method was used in addition to the chemical methods of identification in the screening programme of new antibiotics. The method consists of evaluation of the effect of the crude antibiotic preparations on microbial forms resistant to various antibiotics. The efficiency of the biological method is shown. It provides more complete and rapid characterization of the properties of the new antibiotics and their rough identification at early screening stages. 相似文献
6.
E A Dyskin T M Mefodovskaia M L Moiseeva L P Tikhonova 《Arkhiv anatomii, gistologii i émbriologii》1976,71(11):105-108
The method of detection of lactate dehydrogenase with some modification was used to study the microcirculatory bed in total preparations of serous membranes and plane sections of organs of any square surface. The employment of non-fixed material, the short time (from 15 to 60 min) necessary to obtain preparations of any size and simultaneous determination of the localization and the degree of the activity of the enzyme--are, to the authors opinion, undoubtful advantages of the given method, which can be an addition to the well-known impregnation method of determination of the microcirculatory bed. 相似文献
7.
L S Tikhonova T I Samedov V Iu Galkin V V Babkov K A Makarov A G Efimov 《Antibiotiki i khimioterapii͡a》1990,35(1):27-30
Immobilization of antibiotics on the surface of electrolytically oxidated titanium was tried. Transfer of the immobilized ampicillin into hardly soluble calcium ampicillate resulted in providing the coating with antimicrobial activity for 5 days. The quantity of the immobilized antibiotic determined polarographically amounted to 6.4.10(-3) mol per 1 m2 of the surface. 相似文献
8.
Oshkin I. Yu. Danilova O. V. Suleimanov R. Z. Tikhonova E. N. Malakhova T. V. Murashova I. A. Pimenov N. V. Dedysh S. N. 《Microbiology》2021,90(5):588-597
Microbiology - The technology of single-cell protein production from natural gas is based on using thermotolerant methanotrophic bacteria with high growth rates on methane. So far, the spectrum of... 相似文献
9.
G Gashi V Mahovlić E Bahtiri F Kurshumliu A Podrimaj-Bytyqi IR Elezaj 《Biotechnic & histochemistry》2013,88(7):496-504
Human papilloma virus (HPV) infection is the main etiological factor for cervical intraepithelial lesions (CIN). An important characteristic of this process is the loss of genome stability. Therefore, it is imperative to use biomarkers of DNA damage caused by genomic instability to identify high risk individuals. We investigated the frequency of micronuclei (MN) in peripheral blood lymphocytes (PBL) of 20 patients, diagnosed as histologically CIN 1 and 10 healthy controls. We also examined the frequency of other nuclear anomalies including nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) in PBL of patients with CIN 1 and healthy controls, and evaluated the benefits of p16INK4a and Ki-67 (p16INK4a/Ki-67) immunohistochemical double staining for identifying cervical squamous cells that express HPV E6/E7 oncogenes. We analyzed the association between the frequency of MN in PBL and the amount of p16INK4a/Ki-67 co-expression in CIN 1 patients to establish genomic instability. Among CIN 1 subjects, 15% exhibited diffuse p16INK4a/Ki-67 co-expression and were considered high positive, 25% of the CIN 1 cases exhibited p16INK4a/Ki-67 co-expression restricted to the lower part of the epithelium and were considered low positive and the remaining 60% of cases were negative. The frequency of MN, NPBs and NBUDs differed significantly among groups. We found a statistically significant positive correlation between p16INK4a/Ki-67 co-expression and the frequency of MN, NPBs and NBUDs in PBL. Our findings demonstrate the efficacy of p16INK4a/Ki-67 double immunostaining for histological samples with CIN 1. MN frequency in PBL might be useful for detecting genomic instability in cases of HPV infection and CIN. 相似文献
10.
Tikhonova E. N. Tarnovetskii I. Yu. Malakhova T. V. Gulin M. B. Merkel A. Yu. Pimenov N. V. 《Microbiology》2020,89(6):740-749
Microbiology - Methane oxidation rates and diversity of methane-oxidizing microorganisms in the upper sediment layers of the Crimean Peninsula coastal regions (Black Sea) were investigated.... 相似文献