全文获取类型
收费全文 | 1221篇 |
免费 | 79篇 |
出版年
2023年 | 3篇 |
2022年 | 3篇 |
2021年 | 19篇 |
2020年 | 7篇 |
2019年 | 7篇 |
2018年 | 26篇 |
2017年 | 18篇 |
2016年 | 27篇 |
2015年 | 36篇 |
2014年 | 50篇 |
2013年 | 71篇 |
2012年 | 107篇 |
2011年 | 86篇 |
2010年 | 40篇 |
2009年 | 44篇 |
2008年 | 93篇 |
2007年 | 85篇 |
2006年 | 79篇 |
2005年 | 73篇 |
2004年 | 76篇 |
2003年 | 70篇 |
2002年 | 71篇 |
2001年 | 14篇 |
2000年 | 12篇 |
1999年 | 20篇 |
1998年 | 9篇 |
1997年 | 8篇 |
1996年 | 5篇 |
1995年 | 5篇 |
1994年 | 11篇 |
1993年 | 10篇 |
1992年 | 9篇 |
1991年 | 9篇 |
1990年 | 9篇 |
1989年 | 9篇 |
1988年 | 9篇 |
1987年 | 9篇 |
1986年 | 5篇 |
1985年 | 10篇 |
1984年 | 6篇 |
1983年 | 8篇 |
1981年 | 3篇 |
1980年 | 7篇 |
1979年 | 4篇 |
1978年 | 4篇 |
1976年 | 2篇 |
1975年 | 3篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有1300条查询结果,搜索用时 28 毫秒
1.
2.
Phosphorylation and Inactivation of Brain Glycogen Synthase by a Multifunctional Calmodulin-Dependent Protein Kinase 总被引:1,自引:1,他引:0
Nobuhiro Inoue Takafumi Iwasa Kohji Fukunaga Yasuhiko Matsukado Eishichi Miyamoto 《Journal of neurochemistry》1987,48(3):981-988
Glycogen synthase was partially purified from canine brain to about 70% purity. The purified enzyme showed differences from the properties of the skeletal muscle enzyme with respect to molecular weights of the holoenzyme and subunit and phosphopeptide mapping. The multifunctional calmodulin-dependent protein kinase from the brain phosphorylated brain glycogen synthase with concomitant inactivation of the enzyme. Although about 1.3 mol of phosphate/mol subunit was maximally incorporated into glycogen synthase, 0.4 mol of phosphate/mol subunit was sufficient for the maximal inactivation of the enzyme. The results indicate that brain glycogen synthase is regulated in a calmodulin-dependent manner similarly to the skeletal muscle enzyme, but that the brain enzyme is different from the skeletal muscle enzyme. 相似文献
3.
Sequestration analysis for RNA polymerase I transcription factors with various deletion and point mutations reveals different functional regions of the mouse rRNA gene promoter. 总被引:7,自引:3,他引:4 下载免费PDF全文
M Nagamine T Kishimoto J Aono H Kato R Kominami M Muramatsu 《Molecular and cellular biology》1987,7(4):1486-1495
4.
Changes in Crassulacean Acid Metabolism of Kalanchoe blossfeldiana by Different Nitrogen Sources 总被引:2,自引:0,他引:2
Kalanchoe blossfeldiana Poelln. cv. Hikan (a Crassulacean acidmetabolism (CAM) plant) was grown in pots containing soil for6 months and then cultured in nutrient solution containing 10mM nitrate or ammonium as a sole nitrogen source for 2 or 3months, under a long-day (16 h) condition. Plant growth was better in the nitrate medium. Leaves of thenitrate-grown plants showed greater diurnal fluctuations intitratable acidity and malate content than those of the ammonium-grownplants. The diurnal patterns in CO2 exchange of nitrate-grownplants were basically similar for both groups, but the amountof net CO2 uptake at night was twice as large in the nitrate-grownplants. The leaves of the nitrate-grown plants had 1.3 to 2.5times higher activities of phosphoenolpyruvate carboxylase (PEPC),phosphofructokinase (PFK) and NAD glycelaldehyde-3-phosphatedehydrogenase (G3PDH). These results indicate that K. blossfeldianagrown in nitrate medium showed more CAM activity than thosein ammonium medium. (Received August 13, 1987; Accepted February 22, 1988) 相似文献
5.
J Nakagawa D von der Ahe D Pearson B A Hemmings S Shibahara Y Nagamine 《The Journal of biological chemistry》1988,263(5):2460-2468
6.
Induction and desensitization of plasminogen activator gene expression by tumor promoters 总被引:15,自引:0,他引:15
J L Degen R D Estensen Y Nagamine E Reich 《The Journal of biological chemistry》1985,260(23):12426-12433
7.
Yohichi Kamata Shunji Kozaki Takafumi Nagai Genji Sakaguchi 《FEMS microbiology letters》1985,26(3):305-309
Abstract Five monoclonal antibodies (MCA; E–8–2, 9–1, 11–2, 12–4, and 13–1) against Clostridium botulinum type E derivative toxin were prepared. Their ELISA titers were higher than or equivalent to that of conventional polyclonal antibody. Three of them (E-8–2, 12–4, and 13–1) possessed the neutralizing activity comparable to that of polyclonal antibody. The results of binding-competition experiments indicated that the monoclonal antibodies bound to different sites on the type E toxin molecule. Immunoblotting analyses demonstrated that E-8–2, 9–1, and 11–2 react to fragment I (heavy chain) of the toxin. By use of these monoclonal antibodies, it may be possible to scrutinize the structure-function relationship of botulinum toxins and cross reactions between type E and F toxins. 相似文献
8.
N-hydroxy-2-naphthylamine (NOH-2NA). an active metabolite of human occupational bladder carcinogens, induced, in V79 Chinese hamster cells. chromosomal aberrations which were suppressed in the presence of catalase and/or superoxide dismutase. The induction of the aberrations was more efficient in a more basic pH in parallel with the generation of hydrogen peroxide from NOH-2NA. The possible role of the oxidation product of NOH-2NA in the induction of the aberrations is discussed. 相似文献
9.
Takafumi Ito Hisashi Murata Yoshihide Yasui Morimasa Matsui Tadashi Sakai Kiyoshi Yamauchi 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1995,667(2)
An high-performance liquid chromatographic method with post-column derivatization has been developed for the simultaneous determination of ascorbic acid (AA) and dehydroascorbic acid (DHAA) in fish tissues. Extracted AA and DHAA were separated by a Shim-pack SCR-101H column within 20 min, reacted with sodium hydroxide containing sodium borohydride and monitored at 300 nm. The detection limits for both AA and DHAA were 0.1 μg/ml. 相似文献
10.