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Cytoplasmic male sterility (CMS), one of the most important traits in crop breeding, has been used for commercial seed production by F1 hybrid cultivars of pepper (Capsicum annuum L.). To develop reliable molecular markers for allelic selection of the Restorer-of-fertility (Rf) gene, which is known to be a major determinant of pollen fertility restoration in peppers, a sequence of approximately 10 kb flanking an RAPD fragment closely linked to the Rf locus was obtained by genome walking. A homology search revealed that this sequence contained an LTR retrotransposon and a non-LTR LINE-like retrotransposon. Sequencing of this Rf-linked region to search for polymorphisms between a dominant and recessive allele revealed 98% nucleotide sequence identity between them. A third polymorphic haplotype of the Rf-linked sequence, which has 94-96% nucleotide sequence identity with the two previously isolated haplotypes, was identified among a large number of breeding lines. Utilizing polymorphic sequences in the haplotypes, PCR markers were developed for selection of particular haplotypes and used to examine the distribution of the haplotypes in diverse breeding lines, cultivars, and C. annuum germplasms. Surprisingly, the third haplotype was the predominant type in C. annuum germplasms, while its frequency in F1 hybrid cultivars was relatively low. Meanwhile, analysis of breeding lines whose Rf allele genotypes and male-sterility phenotypes were already known revealed that the third haplotype was mainly present in exotic breeding lines that cause unstable male-sterility when combined with sterile cytoplasms. 相似文献
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Woong-Ki Min Sunggil Kim Soon-Kee Sung Byung-Dong Kim Sanghyeob Lee 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(7):1289-1299
Cytoplasmic male sterility (CMS), one of the most important traits in crop breeding, is used for commercial F1-hybrid seed production in peppers (Capsicum annuum L.). A nuclear gene, Restorer-of-fertility (Rf), can induce normal pollen production in CMS plants resulting in fertility. Since the first report of fertility restoration
in peppers, various inheritance modes have been suggested, including the presence of a third haplotype of the locus. The pepper
Rf gene has not been cloned, and calculated genetic distances of linked markers have varied between research groups. A more
precise allelic test and additional genetic mapping are needed to accurately select recombinants for use in marker-assisted
backcrossing (MAB). Therefore, the reliability and application of these markers for allelic selection of the Rf gene was tested. Two different F2 populations, Buja and Tamna, were used for the construction of a linkage map. From these linkage groups, a new closely linked
flanking marker of the Rf gene were identified. Previous allelic testing revealed the existence of a third haplotype, Rfls
7701
, which can function as dominant (Rf) or recessive (rf). In a previous report, Rfls
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was considered to be linked to unstable male sterility (MS). However, our results suggest that unstable MS was induced by
a gene residing at another locus rather than by Rfls
7701
haplotype-linked allele. 相似文献
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Kim S Song YH Lee JY Choi SR Dhandapani V Jang CS Lim YP Han T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(7):1183-1192
Inheritance of resistance to downy mildew (Hyaloperonospora parasitica) in Chinese cabbage (Brassica rapa ssp. pekinensis) was studied using inbred parental lines RS1 and SS1 that display strong resistance and severe susceptibility, respectively. F(1), F(2), and BC(1)F(1) populations were evaluated for their responses to downy mildew infection. Resistance to downy mildew was conditioned by a single dominant locus designated BrRHP1. A random amplified polymorphic DNA (RAPD) marker linked to BrRHP1 was identified using bulked segregant analysis and two molecular markers designated BrPERK15A and BrPERK15B were developed. BrPERK15B was polymorphic between the parental lines used to construct the reference linkage map of B. rapa, allowing the mapping of the BrRHP1 locus to the A1 linkage group. Using bacterial artificial chromosome clone sequences anchored to the A1 linkage group, six simple polymerase chain reaction (PCR) markers were developed for use in marker-assisted breeding of downy mildew resistance in Chinese cabbage. Four simple PCR markers flanking the BrRHP1 locus were shown to be collinear with the long-arm region of Arabidopsis chromosome 3. The two closely linked flanking markers delimit the BrRHP1 locus within a 2.2-Mb interval of this Arabidopsis syntenic region. 相似文献
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Discovery of a novel cytoplasmic male-sterility and its restorer lines in radish (<Emphasis Type="Italic">Raphanus sativus</Emphasis> L.) 总被引:2,自引:2,他引:0
Lee YP Park S Lim C Kim H Lim H Ahn Y Sung SK Yoon MK Kim S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(6):905-913
A male-sterile (MS) radish (Raphanus sativus L.) was found in an accession collected from Uzbekistan. Unlike Ogura MS radishes in which no pollen grain is typically visible
during anthesis, a small number of pollen grains stuck together in the dehiscing anthers was observed in the newly identified
MS radish. Fluorescein diacetate tests and scanning electron micrographs showed that pollen grains in the new MS radish were
severely deformed and non-viable. Cytological examination of pollen development stages showed a clear difference in the defective
stage from that seen in Ogura male-sterility. Reciprocal cross-pollination with diverse male-fertile lines indicated that
pollen grains of the new MS radish were completely sterile, and the female organs were fully fertile. When the new MS radish
and Ogura MS lines were cross-pollinated with a set of eight breeding lines, all F1 progeny originating from crosses with the new MS radish were male-sterile. In contrast, most of the F1 progeny resulting from crosses with Ogura MS lines were male-fertile. These results demonstrated that factors associated
with induction of the newly identified male-sterility are different from those of Ogura male-sterility. The lack of restorer
lines for the newly identified male-sterility led us to predict that it might be a complete cytoplasmic male-sterility without
restorer-of-fertility genes in nuclear genomes. However, cross-pollination with more diverse radish germplasm identified one
accession introduced from Russia that could completely restore fertility, proving the existence of restorer-of-fertility gene(s)
for the new male-sterility. Meanwhile, the PCR amplification profile of molecular markers for the classification of radish
mitochondrial genome types revealed that the new MS radish contained a novel mitotype. 相似文献