全文获取类型
收费全文 | 248篇 |
免费 | 22篇 |
出版年
2022年 | 4篇 |
2021年 | 13篇 |
2020年 | 7篇 |
2019年 | 9篇 |
2018年 | 4篇 |
2017年 | 7篇 |
2016年 | 10篇 |
2015年 | 16篇 |
2014年 | 18篇 |
2013年 | 20篇 |
2012年 | 14篇 |
2011年 | 23篇 |
2010年 | 8篇 |
2009年 | 6篇 |
2008年 | 7篇 |
2007年 | 9篇 |
2006年 | 13篇 |
2005年 | 12篇 |
2004年 | 8篇 |
2003年 | 8篇 |
2002年 | 7篇 |
2001年 | 14篇 |
2000年 | 11篇 |
1999年 | 1篇 |
1998年 | 4篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1986年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
排序方式: 共有270条查询结果,搜索用时 31 毫秒
1.
2.
Primary structure and properties of the Na+/glucose symporter (Sg1S) of Vibrio parahaemolyticus. 下载免费PDF全文
R I Sarker W Ogawa T Shimamoto T Shimamoto T Tsuchiya 《Journal of bacteriology》1997,179(5):1805-1808
Previously, we cloned and sequenced a DNA fragment from Vibrio parahaemolyticus and found four open reading frames (ORFs). Here, we clearly demonstrate that one of the ORFs, ORF1, is the gene (sglS) encoding a Na+/glucose symporter (SglS). We characterize the Na+/glucose symporter produced in Escherichia coli mutant (JM1100) cells which lack original glucose transport activity and galactose transport activity. We also show that phlorizin, a potent inhibitor of the SGLT1 Na+/glucose symporter of animal cells, inhibited glucose transport, but not galactose transport, via the SglS system. 相似文献
3.
Ahammad Ishtiaque Sarker Md. Rafiul Islam Khan Akib Mahmud Islam Sohidul Hossain Mahmud 《International journal of peptide research and therapeutics》2020,26(4):1923-1938
International Journal of Peptide Research and Therapeutics - Overexpression of ERBBB family of receptors (ERBB1, ERBB2, ERBB3 and ERBB4) has been found to be hyper-activated in a number of... 相似文献
4.
Bonanomi Giuliano Zotti Maurizio Cesarano Gaspare Sarker Tushar C. Saulino Luigi Saracino Antonio Idbella Mohamed Agrelli Diana D’Ascoli Rosaria Rita Angelo Adamo Paola Allevato Emilia 《Plant and Soil》2021,460(1-2):263-280
Plant and Soil - Data about woody debris (WD) decomposition are very scarce for the Mediterranean basin. The specific aim of this work is to explore the relationships between WD traits with the... 相似文献
5.
Majid Mohammadhosseini Claudio Frezza Alessandro Venditti Satyajit D. Sarker 《化学与生物多样性》2021,18(11):e2100317
The aim of this review article is to present, for the first time, an appraisal of the phytochemical, ethnobotanical and pharmacological data on Bunium species. The literature search was conducted using the Scopus, Google Scholar and PubMed databases. The genus Bunium has been found to produce both essential oil (EO), mainly comprising monoterpenes and sesquiterpenes, and non-volatile components mainly coumarins and flavonoids. There are several pharmacological activities associated with the Bunium species, especially antioxidant, antibacterial and antifungal properties. The chemotaxonomic appraisal of the phytochemical pattern of the genus is in sink with the current classification of the family. Moreover, this review confirms the significant ethnobotanical and pharmacological potential of different Bunium species. 相似文献
6.
Suchana R. Sarker Jen McComb Treena I. Burgess Giles E.St.J. Hardy 《Fungal biology》2021,125(6):477-484
Analysis of soil samples using High Throughput Sequencing (HTS) frequently detects more Phytophthora species compared with traditional soil baiting methods. This study investigated whether differences between species in the timing and abundance of sporangial production and zoospore release could be a reason for the lower number of species isolated by baiting. Stems of Eucalyptus marginata were inoculated with ten Phytophthora species (P. nicotianae, P. multivora, P. pseudocryptogea, P. cinnamomi, P. thermophila, P. arenaria, P. heveae, P. constricta, P. gondwanensis and P. versiformis), and lesioned sections for each species were baited separately in water. There were significant differences between species in timing of sporangia production and zoospore release. P. nicotianae, P. pseudocryptogea, P. multivora and P. thermophila released zoospores within 8–12 h and could be isolated from lesioned baits within 1–2 days. In contrast, P. constricta did not produce zoospores for over 48 h and was only isolated 5–7 days after baiting. P. heveae and P. versiformis did not produce zoospores and were not recovered from the baits. When species were paired in the same baiting tub, those that produced zoospores in the shortest time were isolated most frequently, while species slow to produce zoospores, or which produced them in lower numbers, were isolated from few baits or not at all. Thus, species differences in the timing of sporangia production and zoospore release may contribute to the ease of isolation of some Phytophthora species when they are present together with other Phytophthora species in an environmental sample. 相似文献
7.
Sumon Kumar Das Shahnawaz Ahmed Farzana Ferdous Fahmida Dil Farzana Mohammod Jobayer Chisti Daniel T. Leung Mohammad Abdul Malek Kaisar Ali Talukder Pradip Kumar Bardhan Mohammed Abdus Salam Abu Syed Golam Faruque Rubhana Raqib 《PloS one》2013,8(4)
Background
Shigellosis continues to be a public health challenge for developing countries, including Bangladesh. The aim of the study is to demonstrate recent changes in Shigella sero-groups and their geographical diversity.Methods
Data were extracted from data archive of four diarrheal disease surveillance systems. A 2% sub sample from urban Dhaka Hospital (2008–2011; n = 10,650), and 10% from urban Mirpur Treatment Centre (2009–2011; n = 3,585), were enrolled systematically; whereas, all patients coming from the Health and Demographic Surveillance System area in rural Matlab (2008–2011; n = 6,399) and rural Mirzapur (2010–2011; n = 2,812) were included irrespective of age, sex, and disease severity. A fresh stool specimen was collected for identification of Shigella spp. Of them, 315 (3%) were positive for Shigella in Dhaka, 490 (8%) from Matlab, 109 (3%) from Mirpur and 369 (13%) from Mirzapur and considered as analyzable sample size.Results
Among all Shigella isolates regardless of age, significant decreases in percentage of S. flexneri over time was observed in Mirpur (55→29%; p value of χ2-for trend = 0.019) and Mirzapur (59→47%; p = 0.025). A non-significant decrease was also seen in Dhaka (58→48%), while in Matlab there was a non-significant increase (73→81%). Similar patterns were observed among under-5 children at all sites. Emergence of S. sonnei was found in Dhaka (8→25%; p<0.001) and Mirpur (10→33%; p = 0.015), whereas it decreased in Mirzapur (32→23%; p = 0.056). The emergence of S. boydii was seen in all ages in Mirzapur [(3→28%; p<0.001); (3→27%; p<0.001)]. On the other hand, we saw non-significant percent reductions in S. boydii in Dhaka [overall (25→16%); under-5 (16→9%)]. Decreasing rates of Shigella dysenteriae were observed in Matlab, Mirpur and Mirzapur; whereas, in Dhaka it remained unchanged.Conclusion and Significance
Emergence of S. sonnei and S. boydii as important infectious diarrhea etiologies and variations in geographical diversity underscore the need for monitoring, with possible implications for vaccine development. 相似文献8.
Jianbo Yang Varsha Singh Boyoung Cha Tian-E Chen Rafiquel Sarker Rakhilya Murtazina Shi Jin Nicholas C. Zachos George H. Patterson C. Ming Tse Olga Kovbasnjuk Xuhang Li Mark Donowitz 《The Journal of biological chemistry》2013,288(23):16960-16974
Na+/H+ exchanger regulatory factor (NHERF) proteins are a family of PSD-95/Discs-large/ZO-1 (PDZ)-scaffolding proteins, three of which (NHERFs 1-3) are localized to the brush border in kidney and intestinal epithelial cells. All NHERF proteins are involved in anchoring membrane proteins that contain PDZ recognition motifs to form multiprotein signaling complexes. In contrast to their predicted immobility, NHERF1, NHERF2, and NHERF3 were all shown by fluorescence recovery after photobleaching/confocal microscopy to be surprisingly mobile in the microvilli of the renal proximal tubule OK cell line. Their diffusion coefficients, although different among the three, were all of the same magnitude as that of the transmembrane proteins, suggesting they are all anchored in the microvilli but to different extents. NHERF3 moves faster than NHERF1, and NHERF2 moves the slowest. Several chimeras and mutants of NHERF1 and NHERF2 were made to determine which part of NHERF2 confers the slower mobility rate. Surprisingly, the slower mobility rate of NHERF2 was determined by a unique C-terminal domain, which includes a nonconserved region along with the ezrin, radixin, moesin (ERM) binding domain. Also, this C-terminal domain of NHERF2 determined its greater detergent insolubility and was necessary for the formation of larger multiprotein NHERF2 complexes. In addition, this NHERF2 domain was functionally significant in NHE3 regulation, being necessary for stimulation by lysophosphatidic acid of activity and increased mobility of NHE3, as well as necessary for inhibition of NHE3 activity by calcium ionophore 4-Br-. Thus, multiple functions of NHERF2 require involvement of an additional domain in this protein. A23187相似文献
9.
Swapan Kumar Sarker Sanjay Saha Sonet Md. Mohasinul Haque Mahmuda Sharmin 《Ecological Research》2013,28(4):553-565
Tarap Hill Reserve, the largest upland reserve of Bangladesh, is situated along the Indo-Burma Biodiversity Hotspot. It encompasses the last remaining patches of natural vegetation in the Northeastern Tarap Mountain System and harbors 87 % of the nationally declared red-listed vascular plant species. Despite requiring high conservation priority, this is one of the least studied reserves in the tropics. In this study, we collected vegetation and soil (eight variables) data from 68 sample plots. We identified the tree communities by cluster analysis and verified them using the multi-response permutation procedure and detrended correspondence analysis. Species richness, diversity, and compositional similarity between the communities were also estimated. In total, 116 tree species representing 69 genera were recorded within the four identified tree community types. Finally, canonical correspondence analysis with associated Monte Carlo permutation tests (499 permutations) was performed to explore the patterns of variation in tree species distribution explained by the soil variables. Soil phosphorus, organic matter content, and pH were most closely correlated with tree compositional variation. Thus, conservation strategies that take into account variations in these influential soil factors may aid in the conservation of trees in the reserve. 相似文献
10.
Sumon Kumar Das Mohammod Jobayer Chisti Sayeeda Huq Mohammad Abdul Malek Lana Vanderlee Guddu Kaur Mohammed Abdus Salam Tahmeed Ahmed Abu Syed Golam Faruque Abdullah Al Mamun 《PloS one》2013,8(8)