排序方式: 共有14条查询结果,搜索用时 15 毫秒
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KB Cullberg T Christiansen SK Paulsen JM Bruun SB Pedersen B Richelsen 《Obesity (Silver Spring, Md.)》2013,21(3):454-460
Background:
Vascular growth is a prerequisite for adipose tissue (AT) development and expansion. Some AT cytokines and hormones have effects on vascular development, like vascular endothelial growth factor (VEGF‐A), angiopoietin (ANG‐1), ANG‐2 and angiopoietin‐like protein‐4 (ANGPTL‐4).Methods:
In this study, the independent and combined effects of diet‐induced weight loss and exercise on AT gene expression and proteins levels of those angiogenic factors were investigated. Seventy‐nine obese males and females were randomized to: 1. Exercise‐only (EXO; 12‐weeks exercise without diet‐restriction), 2. Hypocaloric diet (DIO; 8‐weeks very low energy diet (VLED) + 4‐weeks weight maintenance diet) and 3. Hypocaloric diet and exercise (DEX; 8‐weeks VLED + 4‐weeks weight maintenance diet combined with exercise throughout the 12 weeks). Blood samples and fat biopsies were taken before and after the intervention.Results:
Weight loss was 3.5 kg in the EXO group and 12.3 kg in the DIO and DEX groups. VEGF‐A protein was non‐significantly reduced in the weight loss groups. ANG‐1 protein levels were significantly reduced 22‐25% after all three interventions (P < 0.01). The ANG‐1/ANG‐2 ratio was also decreased in all three groups (P < 0.05) by 27‐38%. ANGPTL‐4 was increased in the EXO group (15%, P < 0.05) and 9% (P < 0.05) in the DIO group. VEGF‐A, ANG‐1, and ANGPTL‐4 were all expressed in human AT, but only ANGPTL‐4 was influenced by the interventions.Conclusions:
Our data show that serum VEGF‐A, ANG‐1, ANG‐2, and ANGPTL‐4 levels are influenced by weight changes, indicating the involvement of these factors in the obese state. Moreover, it was found that weight loss generally was associated with a reduced angiogenic activity in the circulation. 相似文献3.
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Kamila Pszon-Bartosz Jesper S. Hansen Karin B. Stibius Jesper S. Groth Jenny Emnéus Oliver Geschke Claus Hélix-Nielsen 《Biochemical and biophysical research communications》2011,406(1):96
Reconstitution of functionally active membrane protein into artificially made lipid bilayers is a challenge that must be overcome to create a membrane-based biomimetic sensor and separation device. In this study we address the efficacy of proteoliposome fusion with planar membrane arrays. We establish a protein incorporation efficacy assay using the major non-specific porin of Fusobacterium nucleatum (FomA) as reporter. We use electrical conductance measurements and fluorescence microscopy to characterize proteoliposome fusion with an array of planar membranes. We show that protein reconstitution in biomimetic membrane arrays may be quantified using the developed FomA assay. Specifically, we show that FomA vesicles are inherently fusigenic. Optimal FomA incorporation is obtained with a proteoliposome lipid-to-protein molar ratio (LPR) = 50 more than 105 FomA proteins could be incorporated in a bilayer array with a total membrane area of 2 mm2 within 20 min. This novel assay for quantifying protein delivery into lipid bilayers may be a useful tool in developing biomimetic membrane applications. 相似文献
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KB Gudmundsdóttir S Sigurdarson J Kristinsson T Eiríksson T Jóhannesson 《Acta veterinaria Scandinavica》2006,48(1):16-5
This study was undertaken in order to examine whether any connection existed between the amounts of iron in forage and the
sporadic occurrence of scrapie observed in certain parts of Iceland. As iron and manganese are considered antagonistic in
plants, calculation of the Fe/Mn ratios was also included by using results from Mn determination earlier performed in the
same samples. Forage samples (n = 170) from the summer harvests of 2001–2003, were collected from 47 farms for iron and manganese
analysis. The farms were divided into four categories: 1. Scrapie-free farms in scrapie-free areas (n = 9); 2. Scrapie-free farms in scrapie-afflicted areas (n = 17); 3. Scrapie-prone farms (earlier scrapie-afflicted, restocked farms) (n = 12); 4. Scrapie-afflicted farms (n = 9). Farms in categories 1 and 2 are collectively referred to as scrapie-free farms. The mean iron concentration in forage samples from scrapie-afflicted farms was significantly higher than in forage samples
from farms in the other scrapie categories (P = 0.001). The mean Fe/Mn ratio in forage from scrapie-afflicted farms was significantly
higher than in forage from scrapie-free and scrapie-prone farms (P < 0.001). The results indicated relative dominance of iron
over manganese in forage from scrapie-afflicted farms as compared to farms in the other categories. Thus thorough knowledge
of iron, along with manganese, in soil and vegetation on sheep farms could be a pivot in studies on sporadic scrapie. 相似文献
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Scanning electron microscopy and gramicidin patch clamp recordings of microvillous receptor neurons dissociated from the rat vomeronasal organ 总被引:2,自引:1,他引:1
Vomeronasal organs from female rats were dissociated and isolated
microvillous receptor neurons were studied. The isolated receptor neurons
kept the typical bipolar shape which they have in situ as observed by
scanning electron microscopy. We applied the perforated patch-clamp
technique using the cation-selective ionophore gramicidin on freshly
isolated and well differentiated receptor neurons. The mean resting
potential was -58+/-14 mV (n=39). The contribution of the sodium pump
current to the resting potential was demonstrated by lowering the K+
concentration in the bath or by application of 100 microM dihydro-ouabain.
The input resistance was in the range of 1-6 GOmega and depolarizing
current pulses of a few pA were sufficient to trigger overshooting action
potentials. In voltage clamp conditions a fast transient sodium inward
current and a sustained outward potassium current were activated by
membrane depolarization. These observations indicate that freshly isolated
vomeronasal receptor neurons of rats can be recorded, using gramicidin,
with little modification of the intracellular content. Their
electrophysiological properties are very similar to those observed in situ.
Four out of eight female vomeronasal receptor cells were depolarized by
diluted rat male urine.
相似文献
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Pszon-Bartosz K Hansen JS Stibius KB Groth JS Emnéus J Geschke O Hélix-Nielsen C 《Biochemical and biophysical research communications》2011,414(1):96-100
The gene for a putative cation calcium exchanger (CCX) from Arabidopsis thaliana, AtCCX5, was cloned and its function was analyzed in yeast. Green fluorescent protein-tagged AtCCX5 expressed in yeast was localized in the plasma membrane and nuclear periphery. The yeast transformants expressing AtCCX5 were created and their growth in the presence of various cations (K+, Na+, Ca2+, Mg2+, Fe2+, Cu2+, Co2+, Cd2+, Mn2+, Ba2+, Ni2+, Zn2+, and Li+) were analyzed. AtCCX5 expression was found to affect the response to K+ and Na+ in yeast. The AtCCX5 transformant also showed a little better growth to Zn2+. The yeast mutant 9.3 expressing AtCCX5 restored growth of the mutant on medium with low K+ (0.5 mM), and also suppressed its Na+ sensitivity. Ion uptake experiments showed that AtCCX5 mediated relatively high-affinity K+ uptake and was also involved in Na+ transport in yeast. Taken together, these findings suggest that the AtCCX5 is a novel transport protein involves in mediating high-affinity K+ uptake and Na+ transport in yeast. 相似文献
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Patrick Royston Friederike M-S Barthel Mahesh KB Parmar Babak Choodari-Oskooei Valerie Isham 《Trials》2011,12(1):1-18