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Dida MM Srinivasachary Ramakrishnan S Bennetzen JL Gale MD Devos KM 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(2):321-332
Restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), expressed-sequenced tag (EST),
and simple sequence repeat (SSR) markers were used to generate a genetic map of the tetraploid finger millet (Eleusine coracana subsp. coracana) genome (2n = 4x = 36). Because levels of variation in finger millet are low, the map was generated in an inter-subspecific F2 population from a cross between E. coracana subsp. coracana cv. Okhale-1 and its wild progenitor E. coracana subsp. africana acc. MD-20. Duplicated loci were used to identify homoeologous groups. Assignment of linkage groups to the A and B genome
was done by comparing the hybridization patterns of probes in Okhale-1, MD-20, and Eleusine indica acc. MD-36. E. indica is the A genome donor to E. coracana. The maps span 721 cM on the A genome and 787 cM on the B genome and cover all 18 finger millet chromosomes, at least partially.
To facilitate the use of marker-assisted selection in finger millet, a first set of 82 SSR markers was developed. The SSRs
were identified in small-insert genomic libraries generated using methylation-sensitive restriction enzymes. Thirty-one of
the SSRs were mapped. Application of the maps and markers in hybridization-based breeding programs will expedite the improvement
of finger millet.
Electronic supplementary material Supplementary material is available in the online version of this article at and is accessible for authorized users. 相似文献
2.
Srinivasachary Gosman N Steed A Simmonds J Leverington-Waite M Wang Y Snape J Nicholson P 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(8):1145-1153
Fusarium head blight (FHB) is an important disease of wheat worldwide. The cultivar Spark is more resistant than most other UK winter
wheat varieties but the genetic basis for this is not known. A mapping population from a cross between Spark and the FHB susceptible
variety Rialto was used to identify quantitative trait loci (QTL) associated with resistance. QTL analysis across environments
revealed nine QTL for FHB resistance and four QTL for plant height (PH). One FHB QTL was coincident with the Rht-1D locus and accounted for up to 51% of the phenotypic variance. The enhanced FHB susceptibility associated with Rht-D1b is not an effect of PH per se as other QTL for height segregating in this population have no influence on susceptibility.
Experiments with near-isogenic lines supported the association between susceptibility and the Rht-D1b allele conferring the semi-dwarf habit. Our results demonstrate that lines carrying the Rht-1Db semi-dwarfing allele are compromised in resistance to initial infection (type I resistance) while being unaffected in resistance
to spread within the spike (type II resistance). 相似文献
3.
Identification of QTLs for resistance to Fusarium head blight, DON accumulation and associated traits in the winter wheat variety Arina 总被引:3,自引:0,他引:3
Draeger R Gosman N Steed A Chandler E Thomsett M Srinivasachary Schondelmaier J Buerstmayr H Lemmens M Schmolke M Mesterhazy A Nicholson P 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(5):617-625
Fusarium head blight (FHB) of wheat has become a serious threat to wheat crops in numerous countries. In addition to loss
of yield and quality, this disease is of primary importance because of the contamination of grain with mycotoxins such as
deoxynivalenol (DON). The Swiss winter cultivar Arina possesses significant resistance to FHB. The objective of this study
was to map quantitative trait loci (QTL) for resistance to FHB, DON accumulation and associated traits in grain in a double
haploid (DH) population from a cross between Arina and the FHB susceptible UK variety Riband. FHB resistance was assessed
in five trials across different years and locations. Ten QTL for resistance to FHB or associated traits were detected across
the trials, with QTL derived from both parents. Very few of the QTL detected in this study were coincident with those reported
by authors of two other studies of FHB resistance in Arina. It is concluded that the FHB resistance of Arina, like that of
the other European winter wheat varieties studied to date, is conferred by several genes of moderate effect making it difficult
to exploit in marker-assisted selection breeding programmes. The most significant and stable QTL for FHB resistance was on
chromosome 4D and co-localised with the Rht–D1 locus for height. This association appears to be due to linkage of deleterious genes to the Rht-D1b (Rht2) semi-dwarfing allele rather than differences in height per se. This association may compromise efforts to enhance FHB resistance
in breeding programmes using germplasm containing this allele. 相似文献
4.
Jagjeet Singh Lore Mandeep Singh Hunjan Pritpal Singh Laetitia Willocquet Srinivasachary Sri Serge Savary 《Journal of Phytopathology》2013,161(4):224-229
Sheath blight, caused by Rhizoctonia solani, is one of the most important rice diseases worldwide especially under irrigated agro‐ecosystems. To date, no rice accession with complete resistance to sheath blight has been reported. However, a number of genotypes with varying levels of resistance have been reported. Twelve genotypes (including mega varieties) viz. Tetep, Jasmine 85, Te‐Qing, Duduruchi, Betichikon, Khatochalani, D‐6766, D‐256, Swarna, Sarju‐52, MTU‐1010 and Samba Mashuri were evaluated for quantitative measurement of partial physiological resistance to sheath blight under controlled conditions using detached tiller method. Three independent experiments, each involving three replications, were conducted. Seven days after inoculation, the following disease variables were measured: number of lesions, lesion length, vertical sheath colonization (VSC) on the tiller, disease severity, relative vertical sheath colonization (RVSC) and survival of the leaf blade. Variation between rice genotypes was observed for all the disease variables. Disease severity and VSC were the two most correlated variables, whereas the number of lesions and mean lesion length were the least correlated variables. The ranking of varieties often differed depending on the disease variable considered. Amongst the genotypes tested, D‐256, Tetep and Jasmin‐85 had the lowest number of lesions and disease severity. Similarly, Tetep and D‐256 showed the lowest levels of RVSC, whilst Jasmine‐85 was found to be intermediate. D‐6766, Samba Mashuri and Betichikon showed the highest levels of disease variables. The fraction of dead leaves ranged from 0.00 to 0.38. No dead leaves were observed in Te‐Qing, Swarna and MTU‐1010. The highest fraction of dead leaves was observed for Betichikon (0.38) followed by Duduruchi and D‐6766 (0.33). Our results suggest that this method in combination with other phenotyping methods could be used to quantify partial resistance to rice sheath blight. 相似文献
5.
Srinivasachary N. Gosman A. Steed T. W. Hollins R. Bayles P. Jennings P. Nicholson 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(4):695-702
Fusarium head blight (FHB) is an important disease of wheat worldwide. Soissons is one of the most resistant varieties grown
in UK. The current study was undertaken to identify QTL for FHB resistance in Soissons and to determine whether the semi-dwarfing
alleles Rht-B1b and Rht-D1b have a similar influence on susceptibility to FHB. A Soissons (Rht-B1b; Rht-D1a) × Orvantis (Rht-B1a; Rht-D1b) doubled haploid (DH) population was assessed for FHB resistance in three trials. Soissons contributed a single, stable major
FHB QTL linked to the Rht-D1 locus. In contrast, the Rht-B1b allele (contributed by Soissons) conferred no negative effect on FHB resistance, even conferring a very minor positive effect
in one trial. The influence of the Rht-B1b and Rht-D1b alleles on FHB resistance was further investigated using both Mercia and Maris Huntsman near-isogenic lines. Under high disease
pressure both Rht-B1b and Rht-D1b significantly decreased Type 1 resistance (resistance to initial infection). However, whilst Rht-D1b has no effect on Type 2 resistance (resistance to spread of the fungus within the spike), Rht-B1b significantly increased Type 2 resistance. Our study demonstrates that the choice of semi-dwarfing gene used in plant breeding
programmes may be a significant consideration where resistance to FHB is an important breeding target. 相似文献
6.
Srinivasachary Dida MM Gale MD Devos KM 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(4):489-499
Finger millet is an allotetraploid (2n = 4x = 36) grass that belongs to the Chloridoideae subfamily. A comparative analysis has been carried out to determine the relationship
of the finger millet genome with that of rice. Six of the nine finger millet homoeologous groups corresponded to a single
rice chromosome each. Each of the remaining three finger millet groups were orthologous to two rice chromosomes, and in all
the three cases one rice chromosome was inserted into the centromeric region of a second rice chromosome to give the finger
millet chromosomal configuration. All observed rearrangements were, among the grasses, unique to finger millet and, possibly,
the Chloridoideae subfamily. Gene orders between rice and finger millet were highly conserved, with rearrangements being limited
largely to single marker transpositions and small putative inversions encompassing at most three markers. Only some 10% of
markers mapped to non-syntenic positions in rice and finger millet and the majority of these were located in the distal 14%
of chromosome arms, supporting a possible correlation between recombination and sequence evolution as has previously been
observed in wheat. A comparison of the organization of finger millet, Panicoideae and Pooideae genomes relative to rice allowed
us to infer putative ancestral chromosome configurations in the grasses.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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