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N I Sorokina I V Miroshnichenko M F Nikonova R Kh Aknazarova M G Mikhna 《Radiobiologiia》1987,27(5):581-585
Irradiation of a mouse thymocyte fraction enriched by T-lymphocyte precursors changes the antigenic phenotype of cells toward the increase of their highly differentiated forms. Similar changes in membrane marker antigens are produced by chemical inductors of differentiation and thymotropin. The changes in the cell phenotype induced by the above agents are associated with both membrane and intragenome rearrangements. The results of the experiments on preventing the expression of some antigens by puromycin and the data on the level of spontaneous genome lesions in thymocyte fractions have prompted an assumption that destabilization of the genome upon irradiation increases DNA injury above some critical level which may serve a stimulus for "sorting out" the most radiosensitive thymocyte fraction. 相似文献
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In studying the main indices that characterize the neurochemical system of biosynthesis and degradation of a dopamine neuromediator, tyrosine hydroxylase-dopamine-monoamine oxidase, in different brain regions 5-6 min, 1 and 18 h after whole-body irradiation with high energy electrons (100 Gy) the authors have revealed a 25-40% inhibition of tyrosine hydroxylase and monoamine oxidase activity, and a 40% increase in the dopamine content of basal ganglia of the brain that control behavioural reactions of the organism. The neurochemical disturbances revealed are involved in the mechanisms of early transient incapacity after irradiation with superhigh doses. 相似文献
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Using methods of G- and C-banding, a study was made of the karyotype of mouse myeloma cell line sp2/0-Ag14. The number of chromosomes varies from 58 to 65, the modal class being 61-62. 50 per cent of chromosomes are rearranged. Normal chromosomes 6, 12 and X were not detected in either examined cell of this line. Among the marker chromosomes there are an isochromosome (19/19), three dicentric markers and one marker with two interstitial C-bands. There is a specific marker t (12; 15) of mouse plasmacytomas in the karyotype sp2/0-Ag14. A possible association of specific translocations and segregations of the normal chromosomes with the phenotype of line sp2/0-Ag14 is discussed. The results obtained may be useful for cytogenetic analysis of hybridomas. 相似文献
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The effects of osmotic pressure on inward sodium current during a change in temperature were investigated during experiments on isolated rat spinal ganglia neurons using techniques of intracellular perfusion and voltage clamping. It was found that the effect of osmotic pressure on the kinetic parameters of sodium current does not depend on temperature over a wide range of 8–40°C; the apparent values of activation energies for the activation and inactivation processes do not dependent on degree of osmolality. Overall findings would appear to indicate that the osmotic pressure effect is actually initiated by association with aqueous transmembrane flux. Opinions are expressed as to the location of the structures through which this aqueous flux passes and of the sodium channel gating mechanism, together which the molecular mechanisms of interaction between these two elements.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 18, No. 4, pp. 518–525, July–August, 1986. 相似文献
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Z A Sorokina 《Ukrainski? biokhimicheski? zhurnal》1984,56(4):413-417
Polypeptide fragments from Na+, K+-ATPase of cattle brain are obtained by the bromocyan-treatment of the protein and subsequent gel filtration via sephadexes; the fragments were reconstructed into bilayer lipid membranes (BLM). Polypeptides of fractions I and II induce cationic and polypeptides of fraction IV--cationic-anionic conductivity of BLM. Neither sodium nor potassium selectivity of BLM modified by protein fragments of fractions I and II was observed. Fluctuations of the modified membranes current are of spasmodic character, ATP and inhibitors of the sodium pump do not affect them. The induction of current fluctuations peculiar to channels into BLM is supposed to be a character of polypeptides obtained after the ATPase splitting but not of the cation-transport system of the sodium pump. 相似文献
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现有的分类系统尚缺乏可用于减少䶄属物种种间鉴定不确定性的形态学鉴别特征。鉴于此,本研究旨在筛选出能够对同域分布的棕背䶄(Myodes rufocanus), 红背䶄(M. rutilus)和堤岸䶄(M. glareolus)进行可靠鉴定的头骨形态指标和方法。为了达到以上目的,本文一方面筛选出能够用于种间鉴别的头骨和齿列形态特征,另一方面提出可用于鉴别以上三个物种的计算公式。对不同研究者提出来用于鉴别以上三个同域分布物种的35个特征进行筛选。头骨形态测量数据的获取需要花费较长的时间,而且结果变异很大。在本研究中,在分析中剔除15个形态特征,20个相对保守的特征被选择用于分析(包括来自头盖骨、牙齿和头骨其它部位的特征)。在20个特征中,有8个特征被选择出来,并使用Statistica 10 计算出判别公式。这些特征使准确鉴定不同个体各自所属种类成为可能。 相似文献