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A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences. 总被引:5,自引:0,他引:5 下载免费PDF全文
A nucleic acid sequence-based amplification system primarily targeting mRNA from the Listeria monocytogenes hlyA gene was developed. This system enabled the detection of low numbers (< 10 CFU/g) of L. monocytogenes cells inoculated into a variety of dairy and egg products after 48 h of enrichment in modified listeria enrichment broth. 相似文献
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Blais Burton W. Turner Geoff Sooknanan Roy Malek Lawrence T. Phillippe Lucille M. 《Biotechnology Techniques》1996,10(3):189-194
A NASBA system amplifying specific sequences of the Listeria monocytogenes hlyA gene and an immunoenzymatic assay for the detection of amplimers was developed. The immunoenzymatic assay utilized a simple microtiter plate format and an anti-RNA:DNA hybrid antibody for the detection of NASBA product (predominantly RNA) hybridized to an immobilized DNA probe. This highly sensitive isothermal amplification and detection system was reactive with genomic DNA from various L. monocytogenes isolates but not with other Listeria or non-Listeria species. 相似文献
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