排序方式: 共有6条查询结果,搜索用时 15 毫秒
1
1.
2.
Somta P Musch W Kongsamai B Chanprame S Nakasathien S Toojinda T Sorajjapinun W Seehalak W Tragoonrung S Srinives P 《Molecular ecology resources》2008,8(5):1155-1157
In this study, we reported the isolation and analysis of new polymorphic microsatellites in mungbean (Vigna radiata (L.) Wilczek). Twelve out of 210 primer pairs screened in 30 mungbean accessions gave polymorphism. The polymorphic markers detected two to three alleles per locus with an average of 2.08. Observed heterozygosity varied from 0 to 0.133, while expected heterozygosity ranged from 0.095 to 0.498. Tests for Hardy-Weinberg equilibrium (HWE) and pairwise linkage disequilibrium of the polymorphic loci revealed that all loci except MB-SSR14 significantly departed from HWE and four pairwise combinations, viz. MB-SSR14 vs. MB-SSR42, MB-SSR42 vs. MB-SSR87, MB-SSR114 vs. MB-SSR121, and MB-SSR175 vs. MB-SSR231 significantly deviated from linkage disequilibrium. The markers are being used to study genetic diversity and genome mapping of mungbean. 相似文献
3.
Sermsiri Chanprame T. M. Kuo J. M. Widholm 《In vitro cellular & developmental biology. Plant》1998,34(1):64-68
Summary Somatic and zygotic embryos of soybean cv. Jack were analyzed for soluble carbohydrate, total lipids, and protein during development.
Zygotic embryos accumulated trace amounts of fructose, galactose, and galactinol., whereas somatic embryos contained only
trace amounts of galactose. Somatic embryos accumulated much higher glucose levels than zygotic embryos. Both somatic and
zygotic embryos contain low levels of sucrose, myoinositol, and pinitol. Raffinose and stachyose accumulated in the late developmental
stages of zygotic embryos, but only stachyose was found to accumulate in the late stage somatic embryos. Zygotic embryos contained
low total lipid levels up to 50 d after flowering (DAF) and then the levels increased to 16% by 55 DAF and 21% at 65 DAF.
Somatic embryos had low levels of total lipids throughout development with the maximum of only 4.7%. Soybean zygotic embryos
contained about 40% protein throughout development, while the protein concentration of somatic embryos decreased from 44%
to 25% as maturation approached. These studies demonstrate that the composition of Jack zygotic embryos is similar to that
described for other cultivars during development while the somatic embryo composition and size is markedly different. The
low somatic embryo germination often noted might be due to the abnormal development as shown by a composition different from
that of mature zygotic embryos. The low concentration of the raffinose series sugars might be especially important factors. 相似文献
4.
Peerasak Srinives Ratanakorn Kitsanachandee Thitaporn Chalee Warunee Sommanas Sontichai Chanprame 《Plant and Soil》2010,335(1-2):423-437
Iron deficiency chlorosis (IDC) is a major problem reducing yield of mungbean in many countries. In this study, we crossed “KPS1”, the most popular Thai mungbean cultivar susceptible to IDC with “NM10-12”, a mungbean line from Pakistan resistant to IDC. Segregation analysis of the F2 population revealed that the resistance is controlled by a major gene (IR) with dominant effect. Two AFLP markers, E-ACT/M-CTA and E-ACC/M-CTG were identified closely linking with the IR gene. The frequencies of these markers were assessed in 241 mungbean accessions from several countries. The accessions could be divided, in relative to total chlorophyll content of the resistant check (NM10-12) and the susceptible check (KPS1), into the resistant group with 125 accessions and the susceptible group with 116 accessions. Among 125 resistant accessions, E-ACT/M-CTA and E-ACC/M-CTG were present in 119 (95%) and 109 (87%) accessions, respectively. Both markers can identify all resistant accessions from England, Indonesia and Pakistan, but only E-ACT/M-CTA linked to all resistant accessions from Australia, India, Iraq, Taiwan and Thailand. Understanding the inheritance and identifying molecular markers linking to the IR gene can help plant breeders to improve this crop for growing in iron-deficient soils. 相似文献
5.
Tanadul Orn-u-ma Noochanong Wilawan Jirakranwong Patiruj Chanprame Sontichai 《Bioprocess and biosystems engineering》2018,41(5):613-619
Bioprocess and Biosystems Engineering - The objective of this study was to enhance biomass and lipid productivity in Chlorella sp. isolate 6-4 by inducing mutagenesis with two growth inhibitors:... 相似文献
6.
Pink-pigmented facultative methylotrophic bacteria (PPFMs) have been found on the surfaces of leaves of most plants tested. We found PPFMs on the leaf surfaces of all 40 plants (38 species) tested and on soybean pods by pressing onto AMS medium with methanol as the sole carbon source. The abundance ranged from 0.5 colony forming unit (cfu) /cm2 to 69.4 cfu/cm2 on the leaf surfaces. PPFMs were found in homogenized leaf tissues of only 4 of the species after surface disinfestation with 1.05% sodium hypochlorite and were rarely found in cultures initiated from surface disinfested Datura innoxia leaves or inside surface disinfested soybean pods. Of 20 antibiotics tested for PPFM growth inhibition, rifampicin was the most effective and of seven others which also inhibited PPFM growth, cefotaxime should be the most useful due to the expected low plant cell toxicity. These antibiotics could be used in concert with common surface sterilization procedures to prevent the introduction or to eliminate PPFM bacteria in tissue cultures. Thus, while PPFMs are present on the surfaces of most plant tissues, surface disinfestation alone can effectively remove them so that uncontaminated tissue cultures can be initiated in most cases. 相似文献
1