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Saksinchai S Suzuki M Lumyong S Ohkuma M Chantawannakul P 《Antonie van Leeuwenhoek》2012,101(3):633-640
During a survey of yeasts associated with raw honey collected in Thailand, two strains of the Zygoascus clade were isolated from the Asian cavity-nesting honeybee Apis cerana and the stingless bee Homotrigona fimbriata. Phylogeny based on 26S rDNA D1/D2 sequences placed these yeasts as members of a clade including Candida bituminiphila, Candida patagonica and Candida polysorbophila. The strains of the two novel species, CBS 12271T and CBS 12270T, respectively, could be unquestionably distinguished from their relatives by rDNA sequences and other taxonomic characteristics.
Therefore, the novel anamorphic species, Candida lundiana sp. nov. (type strain CBS 12271T = JCM 16823T) and Candida suthepensis sp. nov. (type strain CBS 12270T = JCM 16822T) are described. 相似文献
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Saksinchai S. Suphantharika M. Verduyn C. 《World journal of microbiology & biotechnology》2001,17(3):307-316
The suitability of using a simple brewer's yeast extract (BYE), prepared by autolysis of complete beer slurry, for growth and sporulation of Bacillus thuringiensis kurstaki was studied in baffled shake flasks. In a standard buffered medium with 2.5% (w/v) glucose and 1% (w/v) brewer's yeast extract, growth of B. t. kurstaki resulted in a low biomass production with considerable byproduct formation, including organic acids and a concomitant low medium pH, incomplete glucose utilization and marginal sporulation, whereas growth in the same medium with a commercial laboratory-grade yeast extract (Difco) resulted in a high biomass concentration, complete glucose utilization, relatively low levels of byproducts and complete sporulation (2.6 × 109 spores/ml). When glucose was left out of the medium, however, growth parameters and sporulation were comparable for BYE and commercial yeast extract, but absolute biomass levels and spore counts were low. Iron was subsequently identified as a limiting factor in BYE. After addition of 3 mg iron sulphate/l, biomass formation in BYE-medium more than doubled, low byproduct formation was observed, and complete sporulation occurred (2.8 × 109spores/ml). These data were slightly lower than those obtained in media with commercial yeast extract (3.6 × 109spores/ml), which also benefited, but to a smaller extent, from addition of iron. 相似文献
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