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SHUCHI SMITA RAVI RAJWANSHI SANGRAM KESHARI LENKA AMIT KATIYAR VISWANATHAN CHINNUSAMY KAILASH CHANDER BANSAL 《Journal of genetics》2013,92(3):363-368
Fruit ripening process is associated with change in carotenoid profile and accumulation of lycopene in tomato (Solanum lycopersicum L.). In this study, we quantified the β-carotene and lycopene content at green, breaker and red-ripe stages of fruit ripening in eight tomato genotypes by using high-performance liquid chromatography. Among the genotypes, lycopene content was found highest in Pusa Rohini and lowest in VRT-32-1. To gain further insight into the regulation of lycopene biosynthesis and accumulation during fruit ripening, expression analysis of nine carotenoid pathway-related genes was carried out in the fruits of high lycopene genotype—Pusa Rohini. We found that expression of phytoene synthase and β-carotene hydroxylase-1 was four and thirty-fold higher, respectively, at breaker stage as compared to red-ripe stage of fruit ripening. Changes in the expression level of these genes were associated with a 40% increase in lycopene content at red-ripe stage as compared with breaker stage. Thus, the results from our study suggest the role of specific carotenoid pathway-related genes in accumulation of high lycopene during the fruit ripening processes. 相似文献
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Scintillation autoradiography was utilized to demonstrate thevariable duration of G2 in Vicia faba root meristems. The ratioof the longest (max G) to the shortest (min ) duration was 5.4,with min being approximately 2.5 h. 相似文献
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