全文获取类型
收费全文 | 2462篇 |
免费 | 166篇 |
出版年
2023年 | 11篇 |
2022年 | 15篇 |
2021年 | 67篇 |
2020年 | 29篇 |
2019年 | 31篇 |
2018年 | 59篇 |
2017年 | 38篇 |
2016年 | 89篇 |
2015年 | 107篇 |
2014年 | 120篇 |
2013年 | 201篇 |
2012年 | 196篇 |
2011年 | 179篇 |
2010年 | 112篇 |
2009年 | 103篇 |
2008年 | 143篇 |
2007年 | 125篇 |
2006年 | 116篇 |
2005年 | 126篇 |
2004年 | 124篇 |
2003年 | 99篇 |
2002年 | 94篇 |
2001年 | 32篇 |
2000年 | 24篇 |
1999年 | 33篇 |
1998年 | 19篇 |
1997年 | 21篇 |
1996年 | 17篇 |
1995年 | 11篇 |
1994年 | 18篇 |
1993年 | 12篇 |
1992年 | 13篇 |
1991年 | 19篇 |
1990年 | 14篇 |
1989年 | 15篇 |
1988年 | 20篇 |
1987年 | 17篇 |
1986年 | 15篇 |
1985年 | 18篇 |
1984年 | 15篇 |
1983年 | 11篇 |
1982年 | 10篇 |
1981年 | 11篇 |
1980年 | 10篇 |
1979年 | 10篇 |
1978年 | 8篇 |
1975年 | 5篇 |
1973年 | 7篇 |
1970年 | 6篇 |
1967年 | 5篇 |
排序方式: 共有2628条查询结果,搜索用时 78 毫秒
1.
Shigeki Takeura Hiizu Aoki Tatsuya Tsurumi Yukihiro Nishiyama Hisashi Fujioka Saiji Yoshii Koichiro Maeno 《Microbiology and immunology》1984,28(4):427-437
Host-dependent restriction of influenza B virus replication in L cells was analysed in comparison with productive infection in MDCK or 1–5C-4 cells. The synthesis and intracellular distribution of virus-specific proteins and the production of cytoplasmic ribonucleoproteins in nonpermissive L cells were similar to those in permissive MDCK cells. However, an electron microscopic study of infected L cells showed neither extracellular virions nor budding virus particles on the cell surface, in contrast to MDCK cells which produced numerous virus particles. PAGE analysis of the plasma membrane isolated from the cells demonstrated no significant difference in the composition of viral polypeptides between permissive 1-5C-4 and nonpermissive L cells. It was noted that the abortiveness of influenza B virus infection in L cells may be due to a defect in host cell function involved in the initiation of virus budding. 相似文献
2.
3.
4.
Chemical modification of a functional arginine residue of rat liver glycine methyltransferase 总被引:1,自引:0,他引:1
Rat liver glycine methyltransferase is inactivated irreversibly by phenylglyoxal in potassium phosphate buffer. The inactivation obeys pseudo-first-order kinetics, and the apparent first-order rate constant for inactivation is linearly related to the reagent concentration. A second-order rate constant of 10.54 +/- 0.44 M-1 min-1 is obtained at pH 8.2 and 25 degrees C. Amino acid analysis shows that only arginine is modified upon treatment with phenylglyoxal. Sodium acetate, a competitive inhibitor with respect to glycine, affords complete protection in the presence of S-adenosylmethionine. Acetate alone has no effect on the rate of inactivation. The value of the dissociation constant for acetate determined from the protection experiment is in good agreement with that obtained by kinetic analysis. Comparison of the amount of [14C]phenylglyoxal incorporated into the protein and the number of arginine residues modified in the presence and absence of protecting ligands indicates that modification of one arginine residue per enzyme subunit eliminates the enzyme activity, and this residue is identified as Arg-175 by peptide analysis. The arginine-modified glycine methyltransferase appears to bind S-adenosylmethionine as the native enzyme does, as seen from quenching of the protein fluorescence by S-adenosylmethionine. These results suggest the requirement of Arg-175 in binding the carboxyl group of the substrate glycine. 相似文献
5.
Kiyotaka Chinzei Kantaro Fujioka Hiroshi Kitazato Itaru Koizumi Tadamichi Oba Motoyoshi Oda Hisatake Okada Toyosaburo Sakai Yoshihiro Tanimura 《Marine Micropaleontology》1987,11(4)
Downcore changes in microfossil assemblages and oxygen isotope ratios in three piston cores recovered from the Northwestern Pacific, off central Japan, show that the subtropical Kuroshio front was located to the south of C-4 core site (Lat. 33° N) during the last glacial. The front then advanced northward, passing over the C-4 site and the C-6 site (34.6° N) at about 13 ka and 10 ka, respectively, and reached the C-1 core site (36° N) at about 7 ka. After 5.5 ka it retreated to the area between the C-1 and C-6 sites. A brief but significant cold event, the readvance of the cold Oyashio Current, is recognized between 11 and 10 ka in the two northern cores, but the current did not reach the southern C-4 site. A contemporaneous cold event is known in the North Atlantic, and the cooling was probably a global phenomenon likely to be associated with lowering of sea level. Contamination of isotopically light water is apparent between 14 and 11 ka in the marked change in isotopic composition of benthic foraminifers. Oxygen isotope ratios of planktonic foraminifers show that prior to the advance of the Kuroshio front, the surface water at these core sites was isotopically lighter than the Kuroshio water at that time. 相似文献
6.
K Hirunagi K Uryu T Fujioka 《Zeitschrift für mikroskopisch-anatomische Forschung》1989,103(4):529-539
Supraependymal cells, fibers and what are presumed to be neuronal bulb-like projections were found in the third ventricle of the domestic chicken with a scanning electron microscope. At least two types of supraependymal cells were found: neuron-like cells and phagocyte-like cells. The former were predominantly seen in the area of the paraventricular organ and infundibular recess. The latter were abundant on the ventricular surface of the median eminence and subfornical organ. Bulb or club-like projections thought to be the dendritic terminals of CSF-contacting neurons were observed in the area of the paraventricular organ and infundibular recess. Similar structures were observed at the preoptic recess as well. The supraependymal neuronal components found in the domestic chicken differed from those of mammals in several respects: 1. the wall of the third ventricle was devoid of supraependymal fibrous plexus except for that of the paraventricular organ; 2. bulb-like projections were abundant in the area of the paraventricular organ; 3. supraependymal neuron-like cells were unipolar or bipolar in appearance. These data underline the dissimilarity of the CSF-contacting neuronal system of birds and mammals. 相似文献
7.
Increased level of apolipoprotein B mRNA in the liver of ventromedial hypothalamus lesioned obese rats 总被引:2,自引:0,他引:2
Y Inui S Kawata Y Matsuzawa K Tokunaga S Fujioka S Tamura T Kobatake Y Keno S Tarui 《Biochemical and biophysical research communications》1989,163(2):1107-1112
The mRNA level of apolipoprotein B (apoB), which is a principal protein component of nascent very low density lipoprotein (VLDL), was determined in parallel with the measurement of acetyl-coenzyme A (Ac-CoA) carboxylase activity in the liver of ventromedial hypothalamus (VMH) lesioned obese rats. Eight weeks after the electrolysis of the bilateral VMH, the level of apoB mRNA in the VMH-lesioned rats was about 1.5-fold higher than that in the sham-operated rats, indicating increased apoB synthesis in the liver of the VMH-lesioned obese rats. The activity of Ac-CoA carboxylase, which is a rate-limiting enzyme for the fatty acid biosynthesis, was about 1.8-fold higher in the VMH-lesioned rats. These observations indicated that VLDL synthesis is increased in the liver of VMH-lesioned obese rats. 相似文献
8.
9.
Summary One hundred twelve human DNA sequences were analyzed with respect to dinucleotide frequency and amino acid composition. The variation in guanine and cytosine (G+C) content revealed: (1) at 2–3 and 3-1 doublet positions CG discrimination is attenuated at high G+C, but TA disfavor is enhanced, and (2) several amino acids are subject to G+C change. These findings have been reported in part for collections of sequences from various species. The present study confirms that in a single organism-the human-the G+C effects do exist. Aspects of the argument that connects G+C with protein thermal stability are also discussed. 相似文献
10.
Cell-surface hydrophobicity of Candida species as determined by the contact-angle and hydrocarbon-adherence methods 总被引:3,自引:0,他引:3
S Minagi Y Miyake Y Fujioka H Tsuru H Suginaka 《Journal of general microbiology》1986,132(4):1111-1115
Cell-surface hydrophobicities of six Candida species were studied by two methods: measurement of the contact angle, and partitioning with aqueous-hydrocarbon (n-octane, n-hexadecane and p-xylene) mixtures. C. tropicalis, C. glabrata and C. krusei adhered better to the hydrocarbons than did C. albicans, C. stellatoidea and C. parapsilosis. Contact angles for the less adherent species were smaller than those for the more adherent species. Thus the two methods gave results that were similar overall and indicated that C. tropicalis, C. glabrata and C. krusei have greater cell-surface hydrophobicities than C. albicans, C. stellatoidea and C. parapsilosis. 相似文献