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1.
Barrière H Rubera I Belfodil R Tauc M Tonnerieux N Poujeol C Barhanin J Poujeol P 《The Journal of membrane biology》2003,193(3):153-170
Volume-sensitive chloride and potassium currents were studied, using the whole-cell clamp technique, in cultured wild-type mouse proximal convoluted tubule (PCT) epithelial cells and compared with those measured in PCT cells from null mutant kcne1 –/– mice. In wild-type PCT cells in primary culture, a Cl– conductance activated by cell swelling was identified. The initial current exhibited an outwardly rectifying current-voltage (I-V) relationship, whereas steady-state current showed decay at depolarized membrane potentials. The ion selectivity was I– > Br– > Cl– >> gluconate. This conductance was sensitive to 1 mM 4,4-Diisothiocyanostilbene-2,2-disulfonic acid (DIDS), 0.1 mM 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) and 1 mM diphenylamine-2-carboxylate (DPC). Osmotic stress also activated K+ currents. These currents are time-independent, activated at depolarized potentials, and inhibited by 0.5 mM quinidine, 5 mM barium, and 10 µM clofilium but are insensitive to 1 mM tetraethylammonium (TEA), 10 nM charybdotoxin (CTX), and 10 µM 293B. In contrast, the null mutation of kcne1 completely impaired volume-sensitive chloride and potassium currents in PCT. The transitory transfection of kcne1 restores both Cl– and K+ swelling-activated currents, confirming the implication of KCNE1 protein in the cell-volume regulation in PCT cells in primary cultures. 相似文献
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3.
Julier B Huguet T Chardon F Ayadi R Pierre JB Prosperi JM Barre P Huyghe C 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(8):1391-1406
In many legume crops, especially in forage legumes, aerial morphogenesis defined as growth and development of plant organs,
is an essential trait as it determines plant and seed biomass as well as forage quality (protein concentration, dry matter
digestibility). Medicago truncatula is a model species for legume crops. A set of 29 accessions of M. truncatula was evaluated for aerial morphogenetic traits. A recombinant inbred lines (RILs) mapping population was used for analysing
quantitative variation in aerial morphogenetic traits and QTL detection. Genes described to be involved in aerial morphogenetic
traits in other species were mapped to analyse co-location between QTLs and genes. A large variation was found for flowering
date, morphology and dynamics of branch elongation among the 29 accessions and within the RILs population. Flowering date
was negatively correlated to main stem and branch length. QTLs were detected for all traits, and each QTL explained from 5.2
to 59.2% of the phenotypic variation. A QTL explaining a large part of genetic variation for flowering date and branch growth
was found on chromosome 7. The other chromosomes were also involved in the variation detected in several traits. Mapping of
candidate genes indicates a co-location between a homologue of Constans gene or a flowering locus T (FT) gene and the QTL
of flowering date on chromosome 7. Other candidate genes for several QTLs are described.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
4.
Thomas?Durand Sophie?Jacob Laura?Lebouil Hassen?Douzane Philippe?Lestaevel Amithys?Rahimian Dimitri?Psimaras Lo?c?Feuvret Delphine?Leclercq Bruno?Brochet Radia?Tamarat Fabien?Milliat Marc?Benderitter Nicolas?Vayatis Georges?No?l Khê?Hoang-Xuan Jean-Yves?Delattre Damien?Ricard Marie-Odile?BernierEmail author 《BMC neurology》2015,15(1):261
Background
Radiotherapy is one of the most important treatments of primary and metastatic brain tumors. Unfortunately, it can involve moderate to severe complications among which leukoencephalopathy is very frequent and implies cognitive deficits such as memory, attention and executive dysfunctions. However, the incidence of this complication is not well established and the risk factors and process are poorly understood. The main objective of the study is to improve knowledge on radio-induced leukoencephalopathy based on pluridisciplinar approaches combining cognitive, biologic, imagery and dosimetric investigations.Method/Design
The EpiBrainRad study is a prospective cohort study including newly diagnosed high grade gliomas patients treated by radiotherapy and concomitant-adjuvant temozolomide chemotherapy. Patients are included between their surgery and first day of radio-chemotherapy, and the follow-up lasts for 3 years after treatment. Cognitive functioning assessments, specific blood biomarkers measures and magnetic resonance imagery are performed at different moment during the follow-up, and a specific dosimetric assessment of organs involved in the beam fields is performed. Firstly, leukoencephalopathy incidence rate will be estimated in this population. Secondly, correlations between cognitive impairments and dosimetry, biomarkers ranges and anomalies on imagery will be analyzed in order to better understand the onset and evolution of cognitive decrement associated with radiotherapy. Furthermore, a new cognitive test, quickly and easily performed, will be studied to determine its sensibility to detect leukoencephalopathy decrement.Discussion
With an original multidisciplinary approach, the EpiBrainRad study aims to improve knowledge on radio-induced leukoencephalopathy in order to improve its early diagnosis and prevention. The main challenge is to preserve quality-of-life after cancer treatments which imply to study the incidence of radiation-induced complications and their associated risk factors.Trial Registration
NCT025441785.
Jennifer F. Knight Vanessa Y.C. Sung Elena Kuzmin Amber L. Couzens Danielle A. de Verteuil Colin D.H. Ratcliffe Paula P. Coelho Radia M. Johnson Payman Samavarchi-Tehrani Tina Gruosso Harvey W. Smith Wontae Lee Sadiq M. Saleh Dongmei Zuo Hong Zhao Marie-Christine Guiot Ryan R. Davis Jeffrey P. Gregg Morag Park 《Cell reports》2018,22(12):3191-3205
6.
Sebastien L'hoste Abderrahmen Chargui Radia Belfodil Christophe Duranton Isabelle Rubera Baharia Mograbi Chantal Poujeol Michel Tauc Philippe Poujeol 《Free radical biology & medicine》2009,46(8):1017-1031
The aim of this study was to characterize the role of CFTR during Cd2+-induced apoptosis. For this purpose primary cultures and cell lines originated from proximal tubules (PCT) of wild-type cftr+/+ and cftr?/? mice were used. In cftr+/+ cells, the application of Cd2+ (5 μM) stimulated within 8 min an ERK1/2-activated CFTR-like Cl? conductance sensitive to CFTRinh-172. Thereafter Cd2+ induced an apoptotic volume decrease (AVD) within 6 h followed by caspase-3 activation and apoptosis. The early increase in CFTR conductance was followed by the activation of volume-sensitive outwardly rectifying (VSOR) Cl? and TASK2 K+ conductances. By contrast, cftr?/? cells exposed to Cd2+ were unable to develop VSOR currents, caspase-3 activity, and AVD process and underwent necrosis. Moreover in cftr+/+ cells, Cd2+ enhanced reactive oxygen species (ROS) production and induced a 50% decrease in total glutathione content (major ROS scavenger in PCT). ROS generation and glutathione decrease depended on the presence of CFTR, since they did not occur in the presence of CFTRinh-172 or in cftr?/? cells. Additionally, Cd2+ exposure accelerates effluxes of fluorescent glutathione S-conjugate in cftr+/+ cells. Our data suggest that CFTR could modulate ROS levels to ensure apoptosis during Cd2+ exposure by modulating the intracellular content of glutathione. 相似文献
7.
Delayed fluorescence as a universal tool for the measurement of circadian rhythms in higher plants 总被引:1,自引:0,他引:1
Peter D. Gould Patrick Diaz Claire Hogben Jelena Kusakina Radia Salem James Hartwell Anthony Hall 《The Plant journal : for cell and molecular biology》2009,58(5):893-901
The plant circadian clock plays an important role in enhancing performance and increasing vegetative yield. Much of our current understanding of the mechanism and function of the plant clock has come from the development of Arabidopsis thaliana as a model circadian organism. Key to this rapid progress has been the development of robust circadian markers, specifically circadian-regulated luciferase reporter genes. Studies of the clock in crop species and non-model organisms are currently hindered by the absence of a simple high-throughput universal assay for clock function, accuracy and robustness. Delayed fluorescence (DF) is a fundamental process occurring in all photosynthetic organisms. It is luminescence-produced post-illumination due to charge recombination in photosystem II (PSII) leading to excitation of P680 and the subsequent emission of a photon. Here we report that the amount of DF oscillates with an approximately 24-h period and is under the control of the circadian clock in a diverse selection of plants. Thus, DF provides a simple clock output that may allow the clock to be assayed in vivo in any photosynthetic organism. Furthermore, our data provide direct evidence that the nucleus-encoded, three-loop circadian oscillator underlies rhythms of PSII activity in the chloroplast. This simple, high-throughput and non-transgenic assay could be integrated into crop breeding programmes, the assay allows the selection of plants that have robust and accurate clocks, and possibly enhanced performance and vegetative yield. This assay could also be used to characterize rapidly the role and function of any novel Arabidopsis circadian mutant. 相似文献
8.
Doxorubicin immunoconjugates containing bivalent,lysosomally-cleavable dipeptide linkages 总被引:4,自引:0,他引:4
Dubowchik GM Radia S Mastalerz H Walker MA Firestone RA Dalton King H Hofstead SJ Willner D Lasch SJ Trail PA 《Bioorganic & medicinal chemistry letters》2002,12(11):1529-1532
Bivalent doxorubicin (DOX)-dipeptides (16a-c) were prepared and conjugated to the monoclonal antibody BR96. The dipeptides are cleaved by lysosomal proteases following internalization of the resulting immunoconjugates. Conjugate 18b demonstrated antigen-specific in vitro tumor cell killing activity (IC(50)=0.2 microM) that was equipotent to DOX with a near doubling of drug molecules/MAb. Size exclusion chromatography showed 18b to be a noncovalent dimer that was formed immediately upon conjugation. 相似文献
9.
Allali S Muller JB Brauner R Lourenço D Boudjenah R Karageorgou V Trivin C Lottmann H Lortat-Jacob S Nihoul-Fékété C De Dreuzy O McElreavey K Bashamboo A 《PloS one》2011,6(10):e24117