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We present a detailed analysis of a regression and an equivalent band-sharing technique for estimating mean within-group relatedness from DNA fingerprint data. First, we show that the relatedness estimate obtained from this technique tends to underestimate the pedigree relatedness in random-mating populations, but that the degree of underestimation will be small unless DNA fingerprint similarity among unrelated individuals is unusually high. We derive the exact asymptotic downward bias as a function of allele frequency and probabilities of identity by descent. We suggest some methods for removing the small downward bias in the regression estimate. Secondly, we show that the technique yields accurate estimates of parent-offspring, full, and half-sibling relatedness in natural populations of the red-winged blackbird Agelaius phoeniceus and indigo bunting Passerina cyanea. 相似文献
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Agglutination of Normal Cells by Plant Lectins following Infection with Nononcogenic Viruses 总被引:4,自引:0,他引:4
MAMMALIAN cells transformed by oncogenic viruses and chemical carcinogens undergo characteristic changes in their surface properties, some of which affect the control of cell multiplication. Certain plant lectins agglutinate transformed cells but not normal cells1–6, which, although possessing binding sites, can only be agglutinated following treatment with proteolytic enzymes3–5. Furthermore, both normal and transformed cells bind equal amounts of lectins, indicating that the increased susceptibility of transformed and trypsinized cells to agglutination is not caused by simple “unmasking” of hidden receptor sites. Nevertheless, the increased susceptibility of normal cells to agglutination following trypsinization may well result from changes occurring in the cell coat material. Since lytic infections with certain nononcogenic viruses10 and various drug treatments11 are known to cause modification of the coat material in normal cells, we were interested to see whether these treatments increased the susceptibility of cells to agglutination by lectins. 相似文献
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Synthesis of Haemoglobin Lepore 总被引:4,自引:0,他引:4
HAEMOGLOBIN Lepore is a class of haemoglobin variants composed of α-chains and hybrids of δ and β-chains, which can be designated as α2(δβ)2. The N-terminal amino-acid sequence of the hybrid chains corresponds to that of δ-chains, while the C-terminal sequence corresponds to that of β-chains. A misplaced synapse of the δ and β gene loci is thought to have occurred during meiosis, followed by a non-homologous cross-over1. Three types are known which differ in the position of the cross-over: Hb Lepore Hollandia2,3, Hb Lepore Baltimore4 and Hb Lepore Boston (Washington)5,6. 相似文献
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NATALIE M. SCHREY AARON W. SCHREY EDWARD J. HEIST JOHN D. REEVE 《Molecular ecology resources》2007,7(5):857-859
We developed microsatellite loci for the southern pine beetle (Dendroctonus frontalis). Twelve microsatellite loci were identified. Eight loci were polymorphic and sufficiently variable in 62 individuals (expected heterozygosity ranged from 0.707 to 0.880) to investigate population structure. All loci conformed to HWE except Dfr‐14, which showed heterozygote excess, and no two loci deviated from linkage equilibrium. The loci were tested for cross‐species amplification in four species of Dendroctonus (D. valens, D. terebrans, D. brevicomis, and D. ponderosae). Seven loci were polymorphic in at least one of the species tested. 相似文献
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The monotypic genus Tanganikallabes, endemic to Lake Tanganyika, is a poorly known member of the family Clariidae. Examination of 142 specimens housed in museum collections has revealed the presence of at least two additional species in this genus. Tanganikallabes alboperca sp. nov. is distinguished from all congeners by the length of its pelvic fins, the presence of a depigmented vertical bar on the opercular margin, and a combination of additional morphometric (pectoral spine length, preanal length, body depth at anus) and meristic (dorsal and anal fin ray counts) characters. Tanganikallabes stewarti sp. nov. is distinguished from other Tanganikallabes species by having a relatively shorter, incomplete lateral line, and shallow body depth at the anus, as well as shorter prepelvic and preanal lengths, and a longer anal fin with a higher number of fin rays. Several morphological characters, as well as genetic data from cytochrome b (mitochondrial DNA) and 18S–ITS1–5.8S–ITS2–28S (ribosomal DNA), indicate that Tanganikallabes constitutes a monophyletic group within the Clariidae and support the recognition of additional species diversity. The monophyly of Tanganikallabes, coupled with the geographical isolation of this group to a single lake satisfy the requirements for its classification as a true species flock, the latest to be described from Lake Tanganyika. © 2012 The Linnean Society of London, Zoological Journal of the Linnean Society, 2012, 165 , 121–142. 相似文献
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In their recent article, Albertin et al. (2009) suggest an autotetraploid origin of 10 tetraploid strains of baker’s yeast (Saccharomyces cerevisiae), supported by the frequent observation of double reduction meiospores. However, the presented inheritance results were puzzling and seemed to contradict the authors’ interpretation that segregation ratios support a tetrasomic model of inheritance. Here, we provide an overview of the expected segregation ratios at the tetrad and meiospore level given scenarios of strict disomic and tetrasomic inheritance, for cases with and without recombination between locus and centromere. We also use a power analysis to derive adequate sample sizes to distinguish alternative models. Closer inspection of the Albertin et al. data reveals that strict disomy can be rejected in most cases. However, disomic inheritance with strong but imperfect preferential pairing could not be excluded with the sample sizes used. The possibility of tetrad analysis in tetraploid yeast offers a valuable opportunity to improve our understanding of meiosis and inheritance of tetraploids. 相似文献
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