全文获取类型
收费全文 | 2988篇 |
免费 | 217篇 |
国内免费 | 1篇 |
专业分类
3206篇 |
出版年
2023年 | 35篇 |
2022年 | 53篇 |
2021年 | 76篇 |
2020年 | 58篇 |
2019年 | 74篇 |
2018年 | 117篇 |
2017年 | 92篇 |
2016年 | 119篇 |
2015年 | 148篇 |
2014年 | 144篇 |
2013年 | 199篇 |
2012年 | 236篇 |
2011年 | 237篇 |
2010年 | 157篇 |
2009年 | 111篇 |
2008年 | 157篇 |
2007年 | 165篇 |
2006年 | 165篇 |
2005年 | 121篇 |
2004年 | 105篇 |
2003年 | 112篇 |
2002年 | 93篇 |
2001年 | 52篇 |
2000年 | 21篇 |
1999年 | 24篇 |
1998年 | 20篇 |
1997年 | 10篇 |
1996年 | 16篇 |
1995年 | 14篇 |
1994年 | 19篇 |
1993年 | 13篇 |
1992年 | 11篇 |
1991年 | 17篇 |
1990年 | 11篇 |
1989年 | 13篇 |
1988年 | 17篇 |
1986年 | 7篇 |
1984年 | 8篇 |
1983年 | 10篇 |
1982年 | 8篇 |
1981年 | 7篇 |
1979年 | 10篇 |
1977年 | 10篇 |
1975年 | 12篇 |
1974年 | 14篇 |
1973年 | 9篇 |
1972年 | 9篇 |
1971年 | 7篇 |
1968年 | 6篇 |
1967年 | 6篇 |
排序方式: 共有3206条查询结果,搜索用时 15 毫秒
1.
2.
Male Great Plains toads, Bufo cognatus, exhibit two mating tactics. At any time, most males give advertisement calls to attract females, while other males, ‘satellites’, remain silent and station themselves near callers in an attempt to intercept females. Females are usually capable of moving through choruses undetected by males. Those females detected by males can avoid being clasped by quickly darting away; but if clasped, can detach the male by inflating. Females initiated amplexus with callers; subsequently calling males mated with 92% of the females and satellites mated with the remaining 8%. Toads employing the satellite tactic associated with males that had longer calls and that were larger, although call duration was the stronger predictor. The percentage of males exhibiting satellite behaviour during a sampling period varied from 0 to 57%. Several hypotheses explaining satellite frequency were explored, including variation in male density, male quality, and operational sex ratio. Of the variables considered, only two aspects of male density (namely, the number of unmated males per m of shoreline and number of calling males per m shoreline) affected satellite frequency. High male density may cause at least some calling males to lose vocal conspicuousness, thereby favouring satellite behaviour. However, male density accounted for only 17% of the observed variation in satellite frequency. This, plus the fact that up to 74% of marked males switched between tactics, suggests that factors such as higher energetic demands of calling may affect satellite frequency. 相似文献
3.
A G Pettigrew F Crepel M Krupa 《Proceedings of the Royal Society of London. Series B, Containing papers of a Biological character. Royal Society (Great Britain)》1988,234(1275):199-218
Neurons of the inferior olive of the rat were studied at different stages of their postnatal (PN) development by using the current clamp technique in slices maintained in vitro. Antidromic and synaptic activation of inferior olivary neurons could be achieved in preparations as young as PN day 2. Neurons at this age already exhibited a variety of ionic conductances which included fast sodium-dependent spikes, high-threshold and low-threshold calcium spikes, potassium-dependent currents, Ca-dependent after-hyperpolarizing potentials (AHPS), and both instantaneous and time-dependent inward rectification at hyperpolarized levels of membrane potential. The two types of Ca-dependent responses recorded in olivary neurons during the first postnatal week were graded with the magnitude of the depolarization imposed on the cells. Furthermore, the high-threshold Ca spikes were only clearly observed during this early period when K conductances were depressed by the injection of caesium into the cells or by bath application of 4-aminopyridine. In contrast, the high-threshold Ca spikes could be obtained without suppression of K currents and were all-or-none in character in some neurons after PN day 8 and in all neurons after PN day 11. The observations suggest that the balance between K and Ca currents changes throughout maturation and is largely in favour of the K current until about the end of the first PN week. At all ages studied, the low-threshold Ca spikes were much less sensitive to the Ca channel blocker cadmium than were the high-threshold Ca spikes. Finally, spontaneous, regular oscillations of the membrane potential were observed for the first time at PN day 16 and were only commonly observed after PN day 19, suggesting a late development of electrotonic coupling between olivary neurons. 相似文献
4.
P Petrás J Zdrazílek H Srámová L Masková 《Journal of hygiene, epidemiology, microbiology, and immunology》1988,32(1):59-69
Tests for enterotoxins A, B, C, D, E and TSST-1 production were carried out on 775 S. aureus strains isolated from various sources (50 mothers and neonates studied periodically, mothers and infants treated for various acute inflammatory conditions, members of hospital staff, environmental swabs) during the period 1981-1983 at a maternity ward chosen for a 3-year systematic study and on additional 97 isolates obtained in 1985 from another maternity ward. This had contributed to a better classification of strains within certain phage type groups. It was found that the distribution of S. aureus types in the particular sub-sets varied, depending on the source of isolates. At the maternity ward followed for 3 years there was a clear-cut trend towards the spread of phage-untypable isolates producing enterotoxin C whereas at ward examined for comparative purposes B enterotoxin producers of phage type 95 were predominant. The tests for enterotoxigenicity has also proved to be useful as the epidemiological marker characterizing the predominantly circulating S. aureus strain. It has been confirmed that the majority role in the spread of maternity-ward-staphylococci is played by the neonates and the factors of hospital environment. 相似文献
5.
6.
7.
8.
Résumé L'ultrastructure des lamelles branchiales et spécialement celle des chloride cells du poisson rouge (Carassius aureus) a été étudiée. Nous avons constaté que du matériel amorphe floconneux, faiblement adiélectronique était attaché aux endroits des creux apicaux. Afin de préciser la nature de ce matériel, nous avons étudié ces structures au microscope électronique avec les techniques suivantes: acide periodique méthènamine d'argent, colorations au fer colloïdal et au bleu d'alcian. Après la réaction à l'acide periodique méthènamine d'argent, de fines précipitations aux endroits des creux apicaux, correspondant au matériel floconneux visible après la fixation au glutaraldéhyde tétroxyde d'osmium, étaient visibles. La coloration au bleu d'alcian révélait des particules fortement colorées formant un film plus ou moins continu à la surface libre des lamelles, sauf aux endroits oò les chloride cells sont en contact avec la surface. Là et notamment dans les 2reux apicaux, du matériel légèrement granuleux, de faible densité, faisait une couche assez épaisse attachée à la membrane cellulaire. Tenant compte des résultats d'autres auteurs et de nos propres observations, nous considérons que la plus grande partie du matériel se trouvant à la surface des chloride cells, et particulièrement dans les creux apicaux, est de type glycoprotéique.
Dédié à Monsieur le Professeur Dr Ernst Horstmann, Hambourg, à l'occasion de son soixantième anniversaire. 相似文献
The ultrastructure of the chloride cells in the gill epithelium of the goldfish
Summary The ultrastructure of the secondary lamellae of the gills and especially that of the chloride cells of Carassius aureus was studied. We found an amorphous, flakey, slightly adielectronic material in the areas of the apical pits. In order to determine the nature of this material, we studied these structures electronmicroscopically applying the periodic acid silver methenamine, colloidal iron and alcian blue methods. The periodic acid silver methenamine reaction, resulted in finely dispersed precipitations which were deposited in the areas of the apical pits and which correspond to the flakey material seen in the ordinary electron micrographs. The alcian blue method reveales strongly stained particles which form a more or less continuous film on the free surface of the lamellae, interrupted only at the level of the chloride cells. In these areas, notably within the apical pits, a rather thick layer of finely granular low-density material is attached to the plasma membrane. In taking into account other studies performed on this subject, as well as our own observations, we consider the material found on the surface of the chloride cells and particularly within their apical pits to be predominantly of glycoproteinous nature.
Dédié à Monsieur le Professeur Dr Ernst Horstmann, Hambourg, à l'occasion de son soixantième anniversaire. 相似文献
9.
Summary Lamellar bodies are described in the non-ciliated epithelial bronchiolar cells of the normal mouse lung. They are constituted of smooth concentric membranes, with a cytoplasmic center. They are related to mitochondria. They seem to belong to smooth endoplasmic reticulum. An origin from Golgi elements is discussed.
Acknowledgment. This work was supported by Grant No 69088 of Conseil de la Recherche Médicale du Québec. 相似文献
10.
Butomus umbellatus L. is a plant species typical of littoral communities of river and stream shores. It can form continuous stands in shallow reservoirs with fluctuating water level. Their expansion is promoted by: (a) intensive vegetative reproduction of plants, (b) crowded sprouting from rhizome fragments on emerged pond bottom, (c) shallow water layer in the year following summer drainage. Expansion of B. umbellatus depends on ploidy level: two cytotypes were found in the Czech and Slovak Republics, differing in their reproductive ability. Seed production of triploids is strongly limited (they are self-incompatible within clones), while diploids can be fully fertile. Nevertheless, even in diploids, the efficiency of seed reproduction under natural conditions is low. Triploids spread by intensive vegetative reproduction, which is decisive for clonal growth of populations and their regeneration after scraping of bottom surface. During seasonal development, maximum of aboveground biomass is produced in early summer, while underground biomass increases till autumn. Growth of the plants is limited by cutting before maximum underground biomass is attained, or by duck grazing. 相似文献