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Perkovskaya G. Yu. Kravchuk Zh. N. Grodzinsky D. M. Dmitriev A. P. 《Russian Journal of Plant Physiology》2004,51(5):609-614
Metabolites of a phytopathogenic fungus Botrytis cinerea Pers. were analyzed for the presence of biotic elicitors. Three groups of elicitors competent in inducing defense responses inAllium cepa cells were identified and partly purified. The recognition of the elicitor signal in onion cells was shown to elevate the concentration of reactive oxygen species (ROS), namely, superoxide anion-radical (O2^{\overset{-}.}) and hydrogen peroxide (22). The intensity of ROS release depended on chemical identity of elicitor and its concentration. The most active ROS production in onion cells was induced by a protein fraction isolated from the medium for fungus culturing. The carbohydrate elicitors extracted from the fungus cytoplasm and cell walls of mycelia were much less effective. The dynamics of ROS generation comprised two stages. The first stage represented fast and low-amplitude changes that peaked in 15 min after the elicitor treatment. The second stage was more durable and extensive; it occurred in 1.5–6 h after the treatment. 相似文献
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E. Yu. Yuzbasheva T. V. Yuzbashev T. K. Konstantinova I. A. Laptev N. I. Perkovskaya S. P. Sineokii 《Applied Biochemistry and Microbiology》2011,47(8):744-753
The closest homologue of the Saccharomyces cerevisiae Flo1p cell wall protein was detected in Yarrowia lipolytica yeast (YALI0C09031p) by the method of genomic analysis, and capacity of its N- and C-domains to expose the Lip2 lipase on
the cell surface was studied. The efficient fixation of the enzyme on the Y. lipolytica cell wall surface was demonstrated. The activity of the cell-bound lipase was 9170 and 3200 units per 1 g of dry solid matter
when using N- and C-domains of the cell wall protein, respectively. At the same time, in the case of immobilization using
the N-domain, approximately 30% of the total lipase activity was detected in the culture medium, whereas when using C-domain
of the cell wall protein YALI0C09031p, practically all lipase was in the immobilized state. Obtained values of the level of
the cell-bound lipase activity considerably exceed previously published data opening a prospect for new technological solutions
which meet industrial needs. 相似文献
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E. Yu. Yuzbasheva T. V. Yuzbashev E. B. Mostova N. I. Perkovskaya S. P. Sineokii 《Applied Biochemistry and Microbiology》2014,50(9):789-801
An analytical review of the current data on the microbial synthesis of biodiesel and prospects of its usage is presented. The technological, biochemical, and genetic aspects of biodiesel production using microorganisms are discussed. 相似文献
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E. Yu. Yuzbasheva P. M. Gotovtsev E. B. Mostova N. I. Perkovskaya M. A. Lomonosova V. V. Butylin S. P. Sineokii R. G. Vasilov 《Applied Biochemistry and Microbiology》2014,50(8):737-749
A review of current work in biodiesel production via enzymatic catalysis has been done. The parameters of the process as determined by laboratories are represented and analyzed. The main factors affecting interesterification are considered. The major types of oils and alcohols used in biodiesel synthesis are listed. The means of lipase enzyme immobilization, including exposure on the cell surface, are discussed. 相似文献
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