On Eosperma oxroadense gen. et sp. nov.: a New Lower Carboniferous Seed from East Lothian

A new petrified platyspermic seed is described from the CementstoneGroup (Tournaisian) of Scotland. It has a generally Lagenostomaleanappearance despite its unusual symmetry, and it is comparedwith some other members of that order. It possesses a well-preservedmegaspore membrane, one of the most notable features of whichis the polygonal markings on the outer surface. Comparison ismade with some megaspores from fossil and living seeds. Thepossible affinity of the seed is discussed.     

SENSORY THRESHOLDS: ESTIMATION FROM DOSE-RESPONSE CURVES

Three methods of threshold estimation are compared for the detection of 1,8-cineole added to Concord grape juice presented in a semi-ascending paired difference design (Lundahl et al. 1986). Method A tests the significance of obtaining a nonzero response, method B determines the transition point of a 2-phase linear model fitted to the data, method C fits a logistic model to the data and defines threshold as the point of maximum curvature, C_max, of the curve. Method A yields interval estimates of thresholds, the midpoints of which systematically decrease with increasing sample size. Methods B and C yield point estimates of thresholds which do not systematically decrease with increasing sample size, are a feature of the dose-response curve, and are similar in value to each other.     

Tracing carbon and oxygen isotope signals from newly assimilated sugars in the leaves to the tree-ring archive

The analysis of δ ¹³C and δ ¹⁸O in tree-ring archives offers retrospective insights into environmental conditions and ecophysiological processes. While photosynthetic carbon isotope discrimination and evaporative oxygen isotope enrichment are well understood, we lack information on how the isotope signal is altered by downstream metabolic processes.
In Pinus sylvestris , we traced the isotopic signals from their origin in the leaf water ( δ ¹⁸O) or the newly assimilated carbon ( δ ¹³C), via phloem sugars to the tree-ring, over a time-scale that ranges from hours to a growing season.
Seasonally, variable ¹³C enrichment of sugars related to phloem loading and transport did lead to uncoupling between δ ¹³C in the tree-ring, and the c _i/ c _a ratio at the leaf level. In contrast, the oxygen isotope signal was transferred from the leaf water to the tree-ring with an expected enrichment of 27‰, with time-lags of approximately 2 weeks and with a 40% exchange between organic oxygen and xylem water oxygen during cellulose synthesis.
This integrated overview of the fate of carbon and oxygen isotope signals within the model tree species P. sylvestris provides a novel physiological basis for the interpretation of δ ¹³C and δ ¹⁸O in tree-ring ecology.     

Mosquito density, biting rate and cage size effects on repellent tests

Mosquito biting rates and the mean duration of protection (in hours) from bites (MDPB) of Aedes aegypti and Anopheles quadrimaculatus , using the repellent 'deet' ( N , N -diethyl-3-methylbenzamide) on a 50 cm² area of healthy human skin, were observed in small (27 l), medium (≈65 l) and large (125 l) cages containing low, medium or high densities of mosquitoes: respectively, 640, 128 or 49 cm³ of cage volume per female. At the initial treatment rate of ≈ 0.4 μl/cm² (1 ml of 25% deet in ethanol on 650 cm² of skin), the MDPB for deet against Ae. aegypti ranged from 4.5 to 6.5 h and was significantly less (5.0 ± 0.8 h) in large cages compared with medium (6.2 ± 0.9 h) and small (6.2 ± 0.8 h) cages, regardless of the density. Against An. quadrimaculatus the MDPB for deet 0.4 μl/cm² was 1.5–8.0 h, less in small (3.7 ± 2.3 h) and large (2.2 ± 1.1 h) cages at medium (3.7 ± 2.3 h) and high (2.5 ± 1.7 h) mosquito densities, and was longest in medium cages (6.2 ± 2.6 h) at low mosquito densities (5.8 ± 2.8 h). With equinoxial photoperiodicity (light on 06.00–18.00 hours) the biting rate was influenced by the time of observation (08.00, 12.00, 16.00 hours) for Ae. aegypti but not for An. quadrimaculatus. For both species, the biting rate was inversely proportional to mosquito density and the MDPB. The shortest MDPBs were obtained in large cages with high densities of mosquitoes and longest protection times occurred in medium sized cages with low mosquito densities.     

Extraintestinal Development of Caryospora simplex (Apicomplexa: Eimeriidae) in Experimentally Infected Mice,Mus musculus1

Developmental stages of Caryospora simplex were found in connective tissue of the cheek, tongue, and nose of Swiss-Webster and C57 BL/6 mice (Mus musculus) from 8 through 70 days after oral inoculation with 50,000 or 250,000 oocysts, or 60,000 free sporocysts of the same species obtained from an Ottoman viper, Vipera xanthina xanthina. The earliest developmental stages were seen on day 8 post-inoculation (PI) and consisted of two types of meronts and gamonts (undifferentiated sexual stages). Gamonts, microgametocytes, macrogametes, and unsporulated oocysts were found on days 10 and 12 PI. Fully sporulated, thin-walled oocysts containing eight sporozoites surrounded by a thin sporocyst membrane were first seen 12 days PI. Monozoic cysts (caryocysts) were first seen 12 days PI and appeared fully viable throughout the duration of the study, 70 days PI. Four mice injected intra-peritoneally with 150,000 free sporozoites and killed 12 days PI contained unsporulated and sporulated oocysts in connective tissues of the cheek, tongue, and nose, suggesting that sporozoites may be carried to the site of infection via the lymphatic/circulatory system. Four cotton rats, Sigmodon hispidus, inoculated orally with 250,000 oocysts all had unsporulated and sporulated oocysts of C. simplex in connective tissue of the cheek, tongue, and nose when killed on day 12 PI, indicating extraintestinal development in the secondary host is not species specific. This is the first report of a heteroxenous coccidium with both asexual and sexual development in the primary (predator) and secondary (prey) hosts.     

Male copulation frequency and female competition for fertilizations in a promiscuous brood parasite, the Pin-tailed Whydah Vidua macroura

Male Pin-tailed Whydahs Vidua macroura copulate infrequently and terminate most courtship displays, even when females are highly receptive. Female sexual interference is aimed at preventing other females from copulating, and is nearly always successful.     

Morphological correlates of nectar production used by honeybees

Abstract.  1. Honeybees foraging on lavender have been shown to choose inflorescences that are larger and have more flowers. If they are selecting optimally then these inflorescences should yield higher net rates of energy gain. The number and distribution of flowers within inflorescences is a complex function of age, however, which might itself influence nectar quality and availability.
2. Sampling of the overnight nectar secretion of visited and unvisited inflorescences showed that younger inflorescences with more flowers produced more sugar per flower and had fewer unproductive flowers than other inflorescences, but the size of the inflorescence had no effects.
3. Overall display size attracted bees to inspect inflorescences, as inflorescences that were inspected but rejected were larger and/or had larger or more bracts than those that were ignored. Bees, however, accepted more productive inflorescences based on different cues: inflorescence age and number of flowers.
4. Inflorescence choice thus appeared to reflect a two-stage decision process based on different morphological criteria at each stage.     

Variable sexual ornaments in scarlet-tufted malachite sunbirds (Nectarinia johnstoni) on Mount Kenya

In order to be elaborated by sexual selection, sexual ornaments must vary perceptibly and genetically among individuals in natural populations. Rather little is known about ornament variation in monogamous species, in which sexual selection should act more weakly than in polygynous species. We report phenotypic variation in feather ornament size (elongated tails and pectoral tufts) and body size in the scarlet-tufted malachite sunbird Nectarinia johnstoni , a monogamous, sexually dimorphic nectarivore of East African alpine zones. Fully-expressed male ornaments are highly significantly more variable (CVs = 12–29%) than are skeletal and wing measures primarily affected by natural selection (CVs = 2 4%). Female sunbirds have pectoral tufts which are significantly (22–25%) smaller than those of adult males, but more variable (CV_s= 21–22%, CV_s= 12–15%), and more variable than body size. Among males with fully-grown ornaments, those with longer tails tend to have longer wings and wider tufts. The high variation in fully-grown ornaments in malachite sunbirds is consistent with the view that the ornaments are condition-dependent sexual signals. Finally, we review studies of feather ornament variation to date, and show that ornaments are much more variable in monogamous than non-monogamous species, apparently due to the relatively weak pressure of sexual selection.