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Joumana El-Turk Omorefe Asemota Juliette Leymarie Christophe Sallaud Stéphane Mesnage Colette Breda Dominique Buffard Adam Kondorosi Robert Esnault 《Gene》1996,170(2):213-216
We constructed an alfalfa cDNA library from mRNA extracted from leaves after infection with Pseudomonas syringae (incompatible interaction). Screening with oligodeoxyribonucleotides designed from regions conserved in all known peroxidases allowed the isolation of four cDNAs (MsprxlA, 1B, 1C and 2). Sequence analysis revealed the presence of open reading frames of 351, 355, 358 and 323 amino acids, respectively, with the characteristic consensus sequences of plant peroxidases. Sequence comparison showed that the Msprx2 product is significantly different from the others and, particularly, lacks a C-terminal propeptide which might be required for vacuolar targeting. 相似文献
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The levels of three essential minerals Ca, Fe and Mg and the extent of their availability were assessed in four commonly eaten
Caribbean tuber crops [dasheen (Xanthosoma spp.), Irish potato (Solanum tuberosum), sweet potato (Ipomoea batatas) and yellow yam (Dioscorea cayenensis)] in their processed and unprocessed states. Calcium was highest in cooked dasheen (5150±50 mg/kg) while Magnesium was highest
in uncooked Irish potato (3600±200 mg/kg). There was no significant loss of calcium from the food samples upon cooking. All
the uncooked food samples displayed higher levels minerals assessed compared to the cooked samples except for cooked Irish
potato that recorded the level of iron (182.25±8.75 mg/kg). Availability of these minerals in the cooked and uncooked tubers
crops upon digestion also showed a similar pattern. In conclusion, the consumption of these tuber crops in the Caribbean may
not be responsible for the reported cases of iron deficiency in the region. However, the␣availability of minerals from these
tuber crops when consumed with other foods (the usual practice in the Caribbean) needs further investigation. 相似文献
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Anthony Uchenna Emeribe Stanley Obialor Anyanwu Idongesit Kokoabasi Isong Uno Remi Bassey Imeobong Joseph Inyang Emmanuel Onyekachukwu Ibeneme Enosakhare Aiyudubie Asemota Zibril Okhormhe Bassey Icha Idris Nasir Abdullahi 《Saudi Journal of Biological Sciences》2021,28(12):6748-6755
BackgroundHypoestes rosea (family: Acanthacea), has been harnessed and utilized for treatment of several ailments. However, there is the paucity of available data on nephrotoxicity associated with this herb. Here, we investigated the phytochemical profile and toxicological effect of H. rosea on Wistar Rats.MethodsTwenty rats (weight range: 75–100 g) were assigned into five study groups, viz; (a) control (without treatment) (b) treatment group 1, orally administered with 50 mg/kg (c) treatment group 2, orally administered with 100 mg/kg (d) treatment group 3, orally administered with 250 mg/kg, and (e) treatment group 4, orally administered with 300 mg/kg of H. rosea, respectively for 28 days of four rats per group. The rats were made unconscious by using oral administration of chloroform. Cardiac punctures were made, and blood samples collected into 10 ml labeled plain container, allowed to clot and spun to harvest serum for determination of sodium, potassium, chloride, bicarbonate, urea and creatinine using colorimetric, back-titrimetric, Urease-Berthelot and Jaffe’s reaction methods respectively. Kidneys of rats were harvested, weighed and immediately fixed in 10% neutral buffered formalin for histological analysis.ResultMean serum sodium (p = 0.049), potassium (p = 0.007), and urea (p < 0.001) levels were significantly higher among the treatment groups compared to controls. Histopathological findings of kidney sections revealed mild glomerular infiltration in treatment groups 2–4. Additionally, sclerosis was observed in groups 3–4. Phytochemical analysis of H. rosea revealed presence of alkaloids, flavonoids, saponins, tannins, terpenoids, steroids and reducing sugars.ConclusionFrom the findings in this study, H. rosea leaf extract causes significant damage to the kidneys of Wistar rats at higher doses. Of which, the damages were dose-dependent in direct proportionality manner. To better determine the safe dosage and ideal duration of consumption, there is the need for further studies on H. rosea. 相似文献
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Marcells A. Omole Lisa Zhou Omowunmi A. Sadik Helen N. Asemota Jason Gilchrist 《Analytical biochemistry》2009,395(1):54-325
This article reports the first electrochemical characterization of pain biomarkers that include arachidonic acid (AA), prostaglandin G2 (PGG2), and cyclooxygenase 2 (COX-2). These biomarkers are mediators of pathophysiology of pain, inflammation, and cell proliferation in cancer. The article also reports the development of an electrochemical immunosensor for monitoring these pain biomarkers. The results revealed that direct electron transfer between AA metabolites and the electrode could be easily monitored and that an enzyme-modified electrode dramatically enhanced bioelectrocatalytic activity toward AA. Cyclic voltammetric analysis of AA revealed a concentration-dependent anodic current with a slope of 2.37 and a limit of detection (LOD) of 0.25 nM. This unique AA/gold electrode electron transfer provides a good electrochemical sensing platform for prostaglandin H2 (PGH2) as the basis for quantitation of pain. An amperometric signal intensity of a COX-2 antibody-modified gold electrode was linear with COX-2 concentration in the range of 0.1-0.5 μg/ml and an LOD of 0.095 μg/ml. The results also revealed a linear correlation of the concentration of PGG2 with an LOD of 0.227 μM. 相似文献
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Cloning and expression of a cDNA encoding a cytoplasmic L5 ribosomal protein from alfalfa (Medicago sativa L.) 总被引:1,自引:0,他引:1
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In this study, the effects of bitter yam sapogenin extract or commercial diosgenin on intestinal disaccharidases and some renal enzymes in diabetic rats were investigated. Diabetic male Wistar rats were fed diets supplemented with 1% sapogenin extract or commercial diosgenin for 3 weeks. Plasma glucose, intestinal disaccharidases and the activities of transaminases, acid phosphatase, glucose-6-phosphatase, ATP citrate lyase, glucose-6-phosphate dehydrogenase and pyruvate kinase were assessed for the level of metabolic changes in the kidney of diabetic rats. Sapogenin extract or commercial diosgenin supplementation resulted in a significant decrease in lactase and maltase activities in all three regions of the intestine compared to the diabetic control group. However, the test diets significantly reduced intestinal sucrase activity in the proximal and mid regions. Test diets supplementation resulted in a significant decrease in the activities of the transaminases compared to the normal and diabetic control groups. The activity of glucose-6-phosphatase was significantly increased while the activities of ATP citrate lyase, pyruvate kinase and glucose-6-phosphate dehydrogenase were significantly reduced in the kidney of the diabetic control rats compared to the normal group. Test diets supplementation did not significantly alter glucose-6-phosphatase, ATP citrate lyase and pyruvate kinase activities compared to the diabetic control. However, there was a significant increase in glucose-6-phosphate dehydrogenase activity toward the normal group. In conclusion, the consumption of bitter yam sapogenin extract or commercial diosgenin demonstrated hypoglycemic properties, which are beneficial in diabetes by reducing intestinal disaccharidases activities; however, bitter yam sapogenin extract may adversely affect the integrity of kidney membrane. 相似文献
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Phytic acid was extracted from sweet potato (Ipomoea batatas) and fed to Wistar rats with or without zinc for 3 weeks. Animals were then sacrificed and bone and faecal minerals were
assessed. The ultra-structure of the bones was examined via scanning electron microscopy. Phytic acid extract or commercial
phytic acid supplemented diets (D + Zn + PE or D + PE) displayed reduced bone calcium levels (101.27 ± 59.11 and 119.27 ± 45.36 g/kg)
compared to the other test groups. Similarly, reduced calcium were observed in the control groups (D + Zn and D) fed formulated
diets with or without zinc supplementation (213.14 ± 15.31 and 210 ± 6.88 g/kg) compared to the other test groups. The group
fed supplemented commercial phytic acid diet (D + CP) demonstrated the lowest femur magnesium (3.72 ± 0.13 g/kg) while the
group fed phytic acid extract supplementation (D + PE) recorded the highest level (4.84 ± 0.26 g/kg) amongst the groups. Femur
iron was highest in the group fed commercial phytic acid supplemented diet (D + CP −115.74 ± 2.41 g/kg) compared to the other
groups. Faecal magnesium levels were significantly higher in the two test groups fed phytic acid extract with or without zinc
(D + Zn + PE or D + PE) compared to all other groups. All the groups which had phytic acid supplemented diets had significantly
thinner bone in the trabecular region, compared to the groups fed formulated diet or zinc supplemented formulated diet (D
or D + Zn). These observations suggest that the consumption of foods high in phytic acid may contribute to a reduction in
the minerals available for essential metabolic processes in rats. 相似文献
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Melissa Powell Andrew O. Wheatley Felix Omoruyi Helen N. Asemota Nadia P. Williams Paula F. Tennant 《Transgenic research》2010,19(3):511-518
Transformation of agricultural crops with novel genes has significantly advanced disease-resistance breeding, including virus
resistance through the expression of virus sequences. In this study, the effects of long-term, repeated exposure to transgenic
papayas carrying the coat protein gene of Papaya ringspot virus and conventional non-transgenic papaya on the histology and selected biochemical parameters of the intestinal tract were
compared. For 3 months, male and female Wistar rats received diets containing transgenic or non-transgenic papaya at twice
the equivalent of the average daily consumption of fresh papayas. Gross and macroscopic appearance of intestinal tissues,
as well as stomach tissues, was comparable (P < 0.05) as were total intestinal bacterial counts and activities of β-glucuronidase. Activities of disaccharidases were not
affected, neither were those of amylase (P < 0.05). Although significant differences were noted in the activity of Ca2+ and Na+/K+ ATPase brush border enzymes, no morphological alteration in the integrity of the intestinal mucosa was found. Overall, negligible
effects on feed intake, body weight, and fecal output were observed (P < 0.05). Taken together, long-term exposure to diets formulated with transgenic papaya did not result in biologically important
unintended effects. 相似文献
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