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Expression of novel lhmlt fusion protein using plant viral vector and study of its anticancer effect
Naseri Zahid Dorani Uliaei Ebrahim Ofoghi Hamideh Davarpanah Seyed Javad 《Plant Cell, Tissue and Organ Culture》2020,143(1):97-108
Plant Cell, Tissue and Organ Culture (PCTOC) - Melittin peptide is the main component of honey bee venom with the cytotoxic and anti-cancer effect which can affect healthy and cancerous cells... 相似文献
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Hamid Moghimi Javad Hamedi Zargham Sepehrizadeh Hamideh Ofoghi 《Annals of microbiology》2013,63(2):669-675
Weeds are a significant part of the pests limiting crop production. Currently, chemical herbicides are widely used for weed control. Environment pollution and the rise of resistant strains highlight the need for new herbicides. Nep1 is a natural bio-herbicide protein which is an effective necrosis stimulant in dicotyledonous weeds. In this study, the cDNA encoding nep1 was isolated form Fusarium oxysporum, cloned and overexpressed in Escherichia coli. The Nep1 inclusion body was purified and refolded. For biological assay, the recombinant Nep1 was applied on Sinapis arvensis, as a chemical herbicide-resistant weed, and on Nicotiana tabacum, as a model plant. Our results show a significant necrosis on the leaves of S. arvensis and N. tabacum after spraying 50 μg/ml of the recombinant protein. 相似文献
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