新疆北部森林生态系统地衣植物生活型分析

Life forms of lichen of forest ecosystem in Northern Xinjiang were researched in the Kanas National Natural Reservation Area.They have been grouped into 6 types:Epipetria,Soil-epipetria,Epiphytia arboricosa,Epiphytia moss,Epiphytis grass and Dry-radicantia,in which the species of Epiphytia arboricosa account for 36.89% of the total species in this area,the species of Epipetria for 22.33% and Epiphytia grass the least.The results show that life forms of lichem at the srea in Northem Xinjiang are relatively rich,which indicates the biodiversity characteristics of lichen.     

DEVELOPMENT AND EVALUATION OF A RAPID ENZYME-LINKED IMMUNOFILTRATION ASSAY (ELIFA) AND AN ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR THE DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN B

An enzyme-linked immunofiltration assay (ELIFA) and a microtitre plate enzyme-linked immunosorbent assay (ELISA) were developed and compared for their ability to detect staphylococcal enterotoxin B (SEB). The double antibody capture format was used for both assays. Factors which improved the sensitivity of the ELIFA system were (1) addition of casein and thimerosal to the antigen dilution buffer; (2) addition of polyethylene glycol (MW 6000) to the detection and conjugate antibody dilution buffers; and (3) washing with diethanolamine buffer prior to addition of the substrate/chromogen. The ELIFA system had a turnaround time of approximately 1 h and a detection limit of 1 ng/mL of purified SEB. The ELISA had a total turnaround time of 21 h, or 3 h using plates pre-coated overnight with the capture antibody. The detection limit of the ELISA for purified SEB was 0.05 ng/mL. The detection limit of SEB in cheese samples spiked with purified enterotoxin and subjected to a simple extraction procedure was 1 ng/mL and 0.1 ng/mL of extract, with the ELIFA and the ELISA, respectively.     

Physiological studies of a hybrid between populations of Dactylis glomerata from contrasting climatic regions. I. Inter population differences

A study of the physiological responses of a hybrid between a North European population and a colchicine-induced autotetraploid of a Portuguese population of Dactylis glomerata subspecies lusitanica showed an intermediate response of the hybrid for many characters. However, the hybrid resembled one or other of the parents in the more favourable expression of certain characters in controlled environments at 20°C, 16 h photoperiod and 8 °C, 8 h photoperiod. Rate of dry-matter production of the hybrid did not differ significantly from that of the northern parent at 20 °C, 16 h and that of the Portuguese parent at 8 °C, 8 h. Leaf growth of the hybrid at 8 °C, 8 h was greater than that of the northern parent and together with the high percentage survival in a freezing test may indicate a role for similar hybrids in the development of potential varieties with improved seasonal distribution of dry-matter production.     

新疆天山西部山脉森林生态系统地衣群落结构的初步研究

 根据多年的研究资料并采用聚类分析方法对分布在天山西部山脉的地衣群落结构进行了研究。结果表明,分布在该地区的地衣种类形成了6种群落:1) 土星猫耳衣（Leptogium saturninum)＋矮石蕊(Cladonia humilis)＋兰灰蜈蚣衣（Physcia caesia）群落;2）黑穗石蕊（Cladonia amaurocr     

Isolation and characterization of microsatellite loci in the sand fly Phlebotomus papatasi (Diptera: Psychodidae)

Phlebotomus papatasi is a proven vector of Leishmania major which is one of the causative agents of cutaneous leishmaniasis in the Old World. Although it has a wide geographical range, its population structure is not yet well understood. In an effort to better understand the population dynamics of this vector, we developed a panel of di‐ and trinucleotide microsatellite markers, using a magnetic bead hybridization enrichment protocol. These microsatellite loci showed three to seven alleles with an expected heterozygosity range between 0.702 and 0.876. The level of polymorphisms found in this study suggests that these microsatellite loci can be used for population analysis of P. papatasi.     

蓝麻黄的组织培养和快速繁殖

1植物名称蓝麻黄(Ephedra glaoca). 2材料类别2000年11月下旬,从新疆托克逊县科委蓝麻黄人工种植实验地取蓝麻黄,连土移栽到大花盆中,放到温室中培养,以其新生幼枝条为外植体. 3培养条件(1)诱导愈伤组织培养基:MS NAA1.5 mg·L-1(单位下同);(2)愈伤组织继代培养基:MS KT 0.05 2,4-D 1.0;(3)诱导芽分化培养基:MS 6-BA 3 IAA 0.2;(4)诱导腋芽培养基:MS 6-BA 0.4.以上培养基均附加3%蔗糖、0.7%琼脂,pH 5.6～5.8.培养温度24～26℃,光照时间15 h·d-1,光照度2 000～3 000 lx.     

禽多杀性巴氏杆菌P1059成熟黏附蛋白的原核表达、纯化和抗原性检测

目的：建立rCPM36在大肠杆菌中的表达体系,纯化表达产物并检测其抗原性。方法：运用PCR方法从禽多杀性巴氏杆菌国际标准株P1059基因组中扩增出编码36kDa成熟黏附蛋白的cpm36基因,构建原核表达载体pQE30-cpm36,转化到大肠杆菌M15中并诱导表达目的蛋白,用镍离子螯合层析柱纯化目的蛋白及制备其抗体,Western印迹分析其抗原性。结果：SDS-PAGE结果显示目标蛋白以可溶性形式表达在大肠杆菌M15细胞质中,其相对分子质量为37kDa,Western印迹结果表明表达蛋白具有良好的抗原性。结论：成功构建出原核表达载体并实现了目的蛋白表达,用镍离子螯合层析柱纯化得到具有抗原性的蛋白,为进一步开展禽多杀性巴氏杆菌黏附因子和保护性抗原的研究奠定基础。     

Cell Expansion and Wall pH in the Fern Regnellidium diphyllum, A Plant Lacking Acidinduced Growth

Petioles of the semi-aquatic fern Regnellidium diphyllum donot show acid growth but low wall pH is a necessary conditionfor maximum rates of IAA-induced cell expansion. Measurementsof wall pH by two indirect methods indicate an unusually lowvalue, in the range pH 4 to 5. This is one to two pH units belowthat estimated for petioles of the semi-aquatic dicotyledonNymphoides peltata, a species in which IAA aand ethylene causegrowth responses very similar to those in Regnellidium but whereacid growth occurs. Having shown previously that fusicoccinenhances proton secretion in both Regnellidium and Nymphoides,we now show that although it causes a reduction in the estimatedapoplast pH to below 4·0 in Regnellidium, cell expansionis not promoted. The FC-induced reduction in pH in Nymphoidesis less and occurs more slowly, but growth is promoted significantly;when IAA and fusicoccin are present together, growth promotionis approximately additive for Nymphoides A model is proposed for Regnellidium in which equilibrium wallpH is maintained at a low value that is optimal for acid growth,the availability of acid-labile sites in the wall being thechief limitation to cell extension. We suggest that this controlmechanism may be widespread for organs without a cuticle, includingroots and the gametophytes of lower plants growing in acidicconditions. Key words: Acid growth, wall pH, fusicoccin, Regnellidium diphyllum, Nymphoides peltata