Oxidants and antioxidants in myocardial infarction (MI): Investigation of ischemia modified albumin,malondialdehyde, superoxide dismutase and catalase in individuals diagnosed with ST elevated myocardial infarction (STEMI) and non-STEMI (NSTEMI)

BackgroundCoronary ischemia can lead to myocardial damage and necrosis. The pathogenesis of cardiovascular diseases often includes increased oxidative stress and decreased antioxidant defense. The study aimed to assess levels of ischemia modified albumin (IMA), malondialdehyde acid (MDA), superoxide dismutase (SOD), and catalase in individuals diagnosed with ST elevated myocardial infarction (STEMI) and non-STEMI.MethodsThe present study prospectively included 50 STEMI patients, 55 NSTEMI patients, and 55 healthy subjects. Only patients who were recently diagnosed with STEMI or NSTEMI were included in this study. IMA, MDA, SOD, and catalase activities were measured spectrophotometrically. Significant coronary artery lesions were determined by angiography.ResultsPatients with ACS had significantly greater IMA and MDA values than the healthy controls (p<0.001). Besides, patients with STEMI had IMA levels that were significantly greater than those of the patients with NSTEMI (p<0.001), while the reverse was true for MDA levels (p<0.001). The healthy controls had the highest levels of SOD and catalase levels, followed by patients with STEMI and patients with NSTEMI, respectively (p<0.001). There was a significant negative correlation among MDA and SOD with catalase levels (r = -0.771 p<0.001 MDA vs catalase; r = -0.821 p<0.001 SOD vs catalase).ConclusionsData obtained in this study reveals that compared to healthy controls, STEMI and NSTEMI patients had increased levels of MDA and IMA and decreased levels of SOD and catalase.     

Reliability-oriented bioinformatic networks visualization

SUMMARY: We present our protein-protein interaction (PPI) network visualization system RobinViz (reliability-oriented bioinformatic networks visualization). Clustering the PPI network based on gene ontology (GO) annotations or biclustered gene expression data, providing a clustered visualization model based on a central/peripheral duality, computing layouts with algorithms specialized for interaction reliabilities represented as weights, completely automated data acquisition, processing are notable features of the system. AVAILABILITY: RobinViz is a free, open-source software protected under GPL. It is written in C++ and Python, and consists of almost 30 000 lines of code, excluding the employed libraries. Source code, user manual and other Supplementary Material are available for download at http://code.google.com/p/robinviz/.     

1H, 13C, and 15N resonance assignment of a 179 residue fragment of hepatitis C virus non-structural protein 5A

Non-structural protein 5A (NS5A) plays an important role in the life cycle of hepatitis C virus. This proline-rich phosphoprotein is organized into three domains. Besides its role in virus replication and virus assembly, NS5A is involved in a variety of cellular regulation processes. Recent studies on domain 2 and 3 revealed that both belong to the class of intrinsically disordered proteins as they adopt a natively unfolded state. In particular, domain 2 together with its vicinal regions is responsible for NS5A’s multiple interactions with other proteins necessary for virus persistence. The low chemical shift dispersion observed for instrinsically disordered proteins presents a challenge for NMR spectroscopy. Here we report sequential resonance assignment of a 179-residue fragment of NS5A, comprising the entire domain 2, using a set of sensitivity and resolution optimized 3D correlation experiments, as well as amino-acid-type editing in ¹H-¹⁵N correlation spectra. Our assignment reveals the presence of several segments with high propensity to form α-helical structure that may be of importance to the function of this protein fragment as a versatile interaction platform.     

Effect of aqueous extract of phyllantus amarus Leaves on implantation and pregnancy in rats

Phyllanthus amarus is a medicinal plant used widely in the treatment of many diseases. It has a long tradition of use in the Hindu Ayurvedic system of medicine and it has long been used as a medicinal agent in cultures around the world. Traditionally, in the Yoruba speaking part of western Nigeria it is used in treating sterility and difficult childbirth. This experiment was therefore designed to scientifically test the effect of aqueous extract of Phyllanthus amarus (AEPA) leaves on implantation and pregnancy. Animals were divided into two groups of 18 rats each after pregnancy has been established. Group I received 0.2mg/100g body weight of AEPA from day 1 of pregnancy. Group II received equal volume of distilled water serving as the control. Six rats from each group were sacrificed on days 6, 8 and 19 respectively. Implantation and pregnancy were assessed. AEPA reduced the time frame for implantation in the treated rats and caused abortion of pregnant rats. Although the aqueous extract of phyllanthus amarus reduces the time frame for implantation, its abortificent effect does not support the traditional claim that it can treat sterility.     

A Generic Model to Solve Tactical Planning Problems in Flexible Manufacturing Systems

This paper is an attempt to develop a generic modeling framework that addresses tactical planning problems of flexible manufacturing systems in a coherent manner. We propose a generic 0-1 mixed integer programming formulation, that integrates batching, loading, and routing problems with their critical aspects related to a system's performance. For this purpose, a thorough analysis is made to determine and relate system components, their attributes, and alternatives together with performance measures specific to tactical planning. This provided the justification to support our argument about generality of the model. A linear programming formulation is provided to approximate the mixed integer formulation proposed so as to overcome the problem's combinatorial complexity. The potential capability of the linear approximation proposed also is demonstrated via a small set of test problems.