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1.
Antônio A. Lins Mesquita Wanderley Gonçlves De Oliveira Rosa M. Taveira Neiva Otto R. Gottlieb 《Phytochemistry》1975,14(3):803-806
The wood of Tovomita pyrifolium (Guttiferae) contains the novel tovopyrifolins A [1,6-dihydroxy-7-methoxy-5-prenyl-6′,6′-dimethylpyrano (2′,3′:3,2)xanthone], B (1,5-dihydroxy-3,4-dimethoxyxanthone) and C (1,3,5-trihydroxy-2-methoxyxanthone) and also the known tovophyllins A and B [structure revised to 1,6-dihydroxy-5-prenyl-6′, 6′-dimethylpyrano(2′,3′:3,2)-6″,6″-dimethylpyrano(2″,3″:7,8)xanthone]. 相似文献
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Jovana Karoline Lima Neiva Leite Luciane Viater Turek Ricardo Lehtonen Rodrigues Souza Luciana da Silva Timossi Ana Claudia Vecchi Osiecki Raul Osiecki Lupe Furtado-Alle 《Gene》2013
Polymorphisms of butyrylcholinesterase (BChE) have been reported to be associated to weight, BMI variance and hypertriglyceridemia in adults and adolescents. The aim of the present study was to investigate the association of −116A (SNP: G/A; rs1126680) and 1914G (SNP: A/G; rs3495) variants of BCHE gene with anthropometric and biochemical variables associated with obesity in population sample of 115 individuals, from Southern Brazil. Participants were grouped in two categories: obese (BMI ≥ 30) and non-obese (BMI < 30). The 1914G allele showed significantly higher frequency in the obese group, and carriers of 1914G allele showed lower mean BChE activity when compared to 1914A carriers (p = 0.006). Higher means of BMI (p = 0.02) and triglyceride (TG; p = 0.01) were found in 1914G carriers (BMI = 27.57kg/m2; TG = 150.8 mg/dL) when compared to 1914A homozygotes (BMI = 25.55 kg/m2; TG = 107.9 mg/dL). Carriers of the −116A allele showed lower mean BChE activity than usual homozygotes, and the −116A variant was found in cis with 1914G (p < 0.0001; D′ = 1). The region of BCHE gene that contains the 1914G mutation site is target of microRNAs (miRs) and the response of BChE to glucocorticoids is especially influenced by these miRs. Therefore, it is possible that the 1914G allele can be interfering in gluconeogenesis, hyperglycemia, lipolysis and body fat distribution. This lower activity may cause an imbalance in lipid metabolism, which may lead to an increased predisposition to obesity and to a lower ability to maintain metabolic homeostasis. 相似文献
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Megalin acts in concert with cubilin to mediate endocytosis of high density lipoproteins 总被引:6,自引:0,他引:6
Hammad SM Barth JL Knaak C Argraves WS 《The Journal of biological chemistry》2000,275(16):12003-12008
Cubilin has recently been shown to function as an endocytic receptor for high density lipoproteins (HDL). The lack of apparent transmembrane and cytoplasmic domains in cubilin raises questions as to the means by which it can mediate endocytosis. Since cubilin has been reported to bind the endocytic receptor megalin, we explored the possibility that megalin acts in conjunction with cubilin to mediate HDL endocytosis. While megalin did not bind to HDL, delipidated HDL, or apoA-I, it was found to copurify with cubilin isolated by HDL-Sepharose affinity chromatography. Cubilin and megalin exhibited coincident patterns of mRNA expression in mouse tissues including the kidney, ileum, thymus, placenta, and yolk sac endoderm. The expression of both receptors in yolk sac endoderm-like cells was inducible by retinoic acid treatment but not by conditions of sterol depletion. Suppression of megalin activity or expression by treatment with either megalin antibodies or megalin antisense oligodeoxynucleotides resulted in inhibition of cubilin-mediated endocytosis of HDL. Furthermore, megalin antisense oligodeoxynucleotide treatment resulted in reduced cell surface expression of cubilin. These data demonstrate that megalin acts together with cubilin to mediate HDL endocytosis and further suggest that megalin may play a role in the intracellular trafficking of cubilin. 相似文献
5.
Vinzent N. Spetzler Nicolas Goldaracena Jan M. Knaak Kristine S. Louis Nazia Selzner Markus Selzner 《Journal of visualized experiments : JoVE》2015,(99)
The success of liver transplantation has resulted in a dramatic organ shortage. Each year, a considerable number of patients on the liver transplantation waiting list die without receiving an organ transplant or are delisted due to disease progression. Even after a successful transplantation, rejection and side effects of immunosuppression remain major concerns for graft survival and patient morbidity. Experimental animal research has been essential to the success of liver transplantation and still plays a pivotal role in the development of clinical transplantation practice. In particular, the porcine orthotopic liver transplantation model (OLTx) is optimal for clinically oriented research for its close resemblance to human size, anatomy, and physiology. Decompression of intestinal congestion during the anhepatic phase of porcine OLTx is important to guarantee reliable animal survival. The use of an active porto-caval-jugular shunt achieves excellent intestinal decompression. The system can be used for short-term as well as long-term survival experiments. The following protocol contains all technical information for a stable and reproducible liver transplantation model in pigs including post-operative animal care. 相似文献
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Karl E Zhang Z Dong Z Neiva KG Soengas MS Koch AE Polverini PJ Núñez G Nör JE 《Cell death and differentiation》2007,14(9):1657-1666
Expression of Bcl-x(L) correlates with the clinical outcomes of patients with cancer. While the role of Bcl-2 in angiogenesis is becoming increasingly evident, the function of Bcl-x(L) in angiogenesis is unclear. Here, we showed that epidermal growth factor (EGF) induces in vitro capillary sprouting and Bcl-x(L) expression in primary endothelial cells. Bcl-x(L)-transduced human dermal microvascular endothelial cells (HDMEC-Bcl-x(L)), but not empty vector control cells, spontaneously organize into capillary-like sprouts. Searching for a mechanism to explain these responses, we observed that Bcl-x(L) induced expression of the pro-angiogenic chemokines CXC ligand-1 (CXCL1) and CXC ligand-8 (CXCL8), and that blockade of CXC receptor-2 (CXCR2) signaling inhibited spontaneous sprouting of HDMEC-Bcl-x(L). Bcl-x(L) led to Bcl-2 upregulation, but Bcl-2 did not upregulate Bcl-x(L), suggesting the existence of a unidirectional crosstalk from Bcl-x(L) to Bcl-2. EGF and Bcl-x(L) activate the mitogen-activated protein kinase/ERK pathway resulting in upregulation of vascular endothelial growth factor (VEGF), a known inducer of Bcl-2 in endothelial cells. Inhibition of VEGF receptor signaling in HDMEC-Bcl-x(L) prevented Bcl-2 upregulation and demonstrated the function of a VEGF-mediated autocrine loop. Bcl-2 downregulation by RNAi blocked CXCL1 and CXCL8 expression downstream of Bcl-x(L), and markedly decreased angiogenesis in vivo. We conclude that Bcl-x(L) functions as a pro-angiogenic signaling molecule controlling Bcl-2 and VEGF expression. These results emphasize a complex interplay between Bcl-2 family members beyond their classical roles in apoptosis. 相似文献
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Stefania Neiva Lavorato Policarpo Ademar Sales Júnior Silvane Maria Fonseca Murta Alvaro José Romanha Ricardo José Alves/ 《Memórias do Instituto Oswaldo Cruz》2015,110(4):566-568
We describe herein the antitrypanosomal activity of 20 novel
1,3-bis(aryloxy)propan-2-amine derivatives. Compounds 2, 4, 6, 12, 15, 16 and 19 were
significantly active against amastigote and trypomastigote forms, with half maximal
inhibitory concentrationvalues in the range of 6-18 µM. In the cytotoxicity tests
against L929 cells, only compound 4 presented selectivity index value above 10,
indicating low toxicity. 相似文献
9.
Mariele Porto Carneiro Le?o Patricia Vieira Tiago Fernando Dini Andreote Welington Luiz de Araújo Neiva Tinti de Oliveira 《Genetics and molecular biology》2015,38(1):86-92
The entomopathogenic fungi of the genus Metarhizium have several
subtilisin-like proteases that are involved in pathogenesis and these have been used
to investigate genes that are differentially expressed in response to different
growth conditions. The identification and characterization of these proteases can
provide insight into how the fungus is capable of infecting a wide variety of insects
and adapt to different substrates. In addition, the pr1A gene has
been used for the genetic improvement of strains used in pest control. In this study
we used quantitative RT-PCR to assess the relative expression levels of the
pr1A gene in M. anisopliae and M.
acridum during growth in different culture conditions and during
infection of the sugar cane borer, Diatraea saccharalis Fabricius.
We also carried out a pathogenicity test to assess the virulence of both species
against D. saccharalis and correlated the results with the pattern
of pr1A gene expression. This analysis revealed that, in both
species, the pr1A gene was differentially expressed under the growth
conditions studied and during the pathogenic process. M. anisopliae
showed higher expression of pr1A in all conditions examined, when
compared to M. acridum. Furthermore, M. anisopliae
showed a greater potential to control D. saccharalis. Taken
together, our results suggest that these species have developed different strategies
to adapt to different growing conditions. 相似文献
10.
Romano E Soares A Proite K Neiva S Grossi M Faria JC Rech EL Aragão FJ 《Genetics and molecular research : GMR》2005,4(2):177-184
Transgene elimination is a poorly studied phenomenon in plants. We made genetic and molecular studies of a transgenic dry bean line immune to bean golden mosaic geminivirus and a soybean line. In both lines, the transgenes were stable during the vegetative phase but were eliminated during meiosis. Due to its potential biotechnological value, this transgenic line was micropropagated by grafting and the vegetative copies were studied for more than two years. More than 300 plants of progeny were obtained during this period, demonstrating that the phenomenon of elimination was consistently repeated and offering an opportunity for detailed study of transgene elimination, including the characterization of the integration sites. Cloning and sequencing of the transgenic loci, reciprocal crosses to untransformed plants, genomic DNA blots, and GUS assays were performed in the transgenic lines. Based on the molecular and genetic characterization, possible mechanisms involved in transgene elimination include intrachromosomal recombination, genetic instability resulting from the tissue culture manipulations, and co-elimination of transgenes, triggered by a process of genome defense. 相似文献