Closing the loop on platinum from catalytic converters: Contributions from material flow analysis and circularity indicators

In this study, material flow analysis (MFA) is applied to quantify and reduce the obstacles for advancing a circular economy (CE) of platinum (Pt) from catalytic converters (CC) in Europe. First, the value chain and related stakeholders are mapped out in an MFA‐like model to both facilitate the assessment of stocks and flows, and get a comprehensive view of potential action levers and resources to close‐the‐loop. Then, through the cross analysis of numerous data sources, two MFA are completed: (i) one general MFA, and (ii) one sector‐specific MFA, drawing a distinction between the fate of Pt from (a) light‐duty vehicles, under the European Union's End of Life Vehicle Directive 2000/EC/53, and (b) heavy‐duty and off‐road vehicles. Key findings reveal a leakage of around 15 tons of Pt outside the European market in 2017. Although approximately one quarter of the losses are due to in‐use dissipation, 65% are attributed to insufficient collection and unregulated exports. Comparing the environmental impact between primary and secondary production, it has been estimated that halving the leakage of Pt during usage and collection could prevent the energetic consumption of 1.3 × 10³ TJ and the greenhouse gases emission of 2.5 × 10² kt CO₂ eq. Through the lens of circularity indicators, activating appropriate action levers to enhance the CE performance of Pt in Europe is of utmost importance in order to secure future production of new generations of CC and fuel cells. Moreover, the growing stockpile of Pt from CC in use indicates the need for better collection mechanisms. Also, the CC attrition during use and associated Pt emissions in the environment appears non‐negligible. Based on the scarce and dated publications in this regard, we encourage further research for a sound understanding of this phenomenon that can negatively impact human health.     

DyRAM: An Active Reliable Multicast Framework for Data Distribution

Group communications (multicast) are foreseen to be one of the most critical yet challenging technologies to meet the exponentially growing demands for data distribution in a large variety of applications of the Internet (such as grid computing, web applications and distributed simulations). When reliability is required, there is no straightforward solutions and meeting the objectives of reliable multicast is not an easy task. Active networks open a new perspective in providing more efficient solutions for the problem of reliability. In this context, routers are able to perform customized computations on the packets flowing through them. In this paper, we propose a receiver-based (replier) local recovery multicast protocol with dynamic repliers elected on a per-packet basis. Designed to provide an efficient reliable multicast service without any cache facilities inside the network, our approach, uses low-overhead active services in routers. The current paper addresses the design, evaluation and the implementation of an efficient and scalable reliable multicast protocol noted DyRAM standing for Dynamic Replier Active reliable multicast.     

Phenolics Composition and Biological Activities Assessment of Leaves,Flowers and Roots Extracts from Erodium glaucophyllum,Erodium hirtum and Erodium guttatum

Biology Bulletin - Erodium glaucophyllum, Erodium hirtum and Erodium guttatum were medicinal herbs from a southern Mediterranean known for its health benefits. There is a strong demand for the find...     

Detection of New Point Mutations of BRCA1 and BRCA2 in Breast Cancer Patients

This study included 20 selected female patients with breast cancer, 30 of their female relatives (sisters and daughters), and 10 healthy females as a control group. Genomic DNA was extracted from peripheral blood lymphocytes of all the subjects, and the polymerase chain reaction was carried out using specific primers for BRCA1 (exons 2 and 8) and BRCA2 (exons 9, 11, and 21). The mutations were detected using a single-strand conformation polymorphism assay and heteroduplex analysis. Finally, the sample variants and their controls were sequenced. Mutations were detected in 44% of the study population, with 18% found in the BRCA1 gene and 26% attributed to BRCA2. Five sequence variants were identified, including two frameshift mutations, one nonsense mutation, and two missense mutations. Therefore, we conclude that germline mutations in two major genes, BRCA1 and BRCA2, may have an important influence on the predisposition and development of familial breast cancer.     

Galvestine-1, a novel chemical probe for the study of the glycerolipid homeostasis system in plant cells

Plant cells are characterized by the presence of chloroplasts, membrane lipids of which contain up to ～80% mono- and digalactosyldiacylglycerol (MGDG and DGDG). The synthesis of MGDG in the chloroplast envelope is essential for the biogenesis and function of photosynthetic membranes, is coordinated with lipid metabolism in other cell compartments and is regulated in response to environmental factors. Phenotypic analyses of Arabidopsis using the recently developed specific inhibitor called galvestine-1 complete previous analyses performed using various approaches, from enzymology, cell biology to genetics. This review details how this probe could be beneficial to study the lipid homeostasis system at the whole cell level and highlights connections between MGDG synthesis and Arabidopsis flower development.     

Effect of total sleep deprivation on egocentric distance estimation following a fatiguing task

The aim of this study was to investigate the effect of a total sleep deprivation (TSD) on the egocentric distance estimation following a fatiguing task (i.e. sprint repetitions). Ten soccer players (age: 22.8 ± 1.3 years; weight: 72. 8 ± 10 .5 kg; height: 1.8 ± 0.03 m) were volunteered to participate in the study. Subjects were invited to estimate different distances in two different sessions (i) following a reference night and (ii) following a night of TSD. The distance estimation consists to perceive three different distances (15, 25, and 35 m) before and after a repeated sprint exercise on a cycloergometer. The results demonstrated a significant effect of TSD on the perception of egocentric distance (F (1.9) = 11.22, p < 0.01). Indeed, the results showed an underestimation (compression of the real distance) of all distances at rest and following the repeated sprint exercise. Additionally, the underestimation was more acute after the fatiguing task. TSD affects the estimation of egocentric distance by soccer players at rest and following fatiguing task.     

miR-203 modulates epithelial differentiation of human embryonic stem cells towards epidermal stratification

The molecular mechanisms controlling the differentiation of human basal keratinocyte stem cells towards the epidermis are well characterized, whereas the earliest process leading to the specification of embryonic stem cells into keratinocytes is still not well understood. MicroRNAs are regulators of many cellular events, but evidence for microRNA acting on the differentiation of human embryonic stem cells into a specific lineage has been elusive. By using our recent protocol for obtaining functional keratinocytes from hESC, we attempted to analyze the role of microRNAs in the early stages of epidermal differentiation. Thus, we identified a set of 5 microRNAs, namely miR-200a, miR-200b, miR-203, miR-205 and miR-429, that are specifically overexpressed during the early stages of the differentiation process. Interestingly, our functional analyses revealed an instrumental role of miR-203, which had been previously shown to play a key role during the formation of the pluristratified epidermis by basal keratinocyte stem cells, in the early keratinocyte commitment. These results highlight the determinant and unique role of miR-203 during the entire process of epidermal development by extending its spectrum of action from the early commitment of embryonic stem cells to ultimate differentiation of the organ.     

Comparison of Immobilized and Free Amyloglucosidase Process in Glucose SyrupsProduction from White Sorghum Starch

Optimum conditions for glucose syrups production from white sorghum were studied through sequential liquefaction and saccharification processes. In the liquefaction process, a maximum dextrose equivalent (DE) of 10.98 % was achieved using 30 % (w/v) of starch and Termamyl ɑ-amylase from Bacillus licheniformis. Saccharification was performed by free and immobilized amyloglucosidase from Rhizopus mold at 1 % (w/v). DE values of 88.32 % and 79.95 % were obtained from 30 % (w/v) of starch with, respectively, free and immobilized enzyme. The immobilized Amyloglucosidase in calcium alginate beads showed reusable capacity for up to 6 cycles with 46 % of the original activity retained. The kinetic behaviour of immobilized and free enzyme gives K_m value of 22.13 and 16.55 mg mL⁻¹ and V_max of 0.69 and 1.61 mg mL⁻¹ min⁻¹, respectively. The hydrolysis yield using immobilized amyloglucosidase were lower than that of the free one. However, it is relevant to reuse enzyme without losing activity in order to trim down the overall costs of enzymatic bioprocesses as starch transformation into required products in industrial manufacturing. Hydrolysis of sorghum starch using immobilized amyloglucosidase represents a promising alternative towards the development of the glucose syrups production process and its utilization in various industries.