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1.
The influence of phosphate nutrition on H ion efflux from the roots of young rape plants 总被引:3,自引:0,他引:3
Changes in pH around the roots of young rape plants were studied using a nutrient film technique which allowed either part
or all of the root system to be subjected to specific nutrient treatments. The rapidity and direction of change of pH was
assessed by embedding absorbing roots in a thin layer of agar containing bromocresol purple. Measurements were also made with
a pH microelectrode placed next to the roots.
Phosphate-fed plants were deprived of phosphate when 14 days old. Patterns of pH changes round the deprived roots were the
same as with phosphate-fed plants until the plants had been deprived of P for three days, when H ion efflux started in the
terminal portions of the roots. The lengths of root producing acid and amounts of H ion both increased as the plants became
more P deficient. Both P fed and P deprived roots produced HCO3 ions but the net amount of HCO3 ion produced by the P deficient roots fell as did nitrate uptake rates. Cation-anion balances measured at the end of the
experiment showed that uptake of all anions and K decreased in the P deprived plants but uptake of Ca and Mg were little altered.
This resulted in a smaller ratio of anions to cations absorbed which was reflected in the reduced HCO3 ion efflux. 相似文献
2.
The effects (and interaction) of two solution concentrations of Mg (50, 500, μM) and two of K (250, 4250 μM) on the growth of micropropagated plants of “F. 12/1” and “Colt” were investigated using a flowing solution culture system.
Magnesium inflow and growth of “Colt” and “F. 12/1” were inhibited to a similar extent by an increased concentration of K
in the nutrient solution. However, the consequences of this inhibition were different. Reduced inflow of Mg in “F. 12/1” caused
Mg deficiency symptoms at high and low concentrations of K, whereas this only occurred with a combination of high K concentration
and low Mg concentration in “Colt”. The distribution of dry matter within the plant was significant in determining susceptibility
to Mg deficiency. Since “F. 12/1” has a smaller root:shoot ratio than Colt it is unable to sustain the same concentration
of Mg in leaves as “Colt” irrespective of external K concentration. The molar ratio of K:Mg in soil solutions should remain
<8.5:1 in order to ensure maximum growth of “F. 12/1” and “Colt”.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
3.
Levels of nitrate reductase activity (N.R.A.) were measured in shoots and roots of P sufficient and P deficient rape plants
and changes in N.R.A. examined in relation to the onset of H ion efflux from the roots. Rates of xylem exudation were measured
and the sap analysed for nitrate, amino-N and phosphate content.
The optimum concentration of phosphate in the leaves for N.R.A. was about 0.7%. Both high and low concentrations of phosphate
within the leaves inhibited N.R.A in those leaves. This inhibition of N.R.A led to the accumulation of nitrate in the older
parts of the shoots of P sufficient plants. Less accumulation of nitrate occurred in the P deficient plants since nitrate
uptake by the plants decreased before any fall in N.R.A.
Xylem exudation rates halved within 18 hours of depriving the plants of phosphate, and, since the composition of the sap remained
constant, this indicated a reduced flux of nitrate into the xylem. The rate of xylem exudation continued to fall and by the
end of the experiment was approximately one tenth of the rate in the P sufficient plants.
The onset of H ion efflux from the terminal portions of the root preceded any effect on N.R.A by 2 days. 相似文献
4.
Investigations into the Role of Sucrose in Potato cv. Estima Microtuber Production in vitro 总被引:1,自引:0,他引:1
Sucrose has been the carbohydrate traditionally used for potatomicrotuber production. Added to nutrient media, sucrose canact solely as a carbon source, or as an osmoticum, or both.Preliminary tests showed that the osmolarity of sucrose solutionswas increased by autoclaving, indicating some breakdown of thesugar. This was taken into consideration in experiments whichinvolved supplementing 4% sucrose media with sucrose, maltose,glucose or fructose, while keeping the osmotic potential ofthe media constant. A medium concentration of about 400 mM withonly sucrose was more suitable for microtuber production thanmedia supplemented with maltose, glucose or fructose. However,a better microtuber yield was obtained when hexoses were addedthan with unsupplemented 4% sucrose media. When glucose wassupplied at concentrations which had the same number of carbonatoms as 8% sucrose, the high osmolarity inhibited microtuberisation.Sugar movement in the tubering plantlet was followed using radio-labelledsucrose, glucose and fructose. The sucrose was translocatedand used at a faster rate than the other sugars, which tendedto remain in the roots of the plantlets. Furthermore, therewas no difference in microtuber production on media to whichthe sucrose was added before or after autoclaving, indicatingthat levels of breakdown were not severe enough to affect theprocess. Therefore, it is concluded that sucrose acts primarilyas a suitable carbon source for uptake and utilization by theplantlets, but, at 8%, it also provides a favourable osmolarityfor the development of microtubers.Copyright 1995, 1999 AcademicPress Solanum tuberosum (L.), potato, microtuber, media, sugar, sucrose, osmolarity, pH 相似文献
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6.
Ruminant livestock turn forages and poor-quality feeds into human edible products, but enteric methane (CH4) emissions from ruminants are a significant contributor to greenhouse gases (GHGs) and hence to climate change. Despite the predominance of pasture-based beef production systems in many parts of Europe there are little data available regarding enteric CH4 emissions from free-ranging grazing cattle. It is possible that differences in physiology or behaviour could influence comparative emissions intensities for traditional and modern breed types depending on the nutritional characteristics of the herbage grazed. This study investigated the role of breed type in influencing CH4 emissions from growing beef steers managed on contrasting grasslands typical of intensive (lowland) and extensive (upland) production systems. Using the SF6 dilution technique CH4 emissions were estimated for a modern, fast-growing crossbred (Limousin cross) and a smaller and hardier native breed (Welsh Black) when grazing lowland perennial ryegrass (high nutritional density, low sward heterogeneity) and semi-improved upland pasture (low/medium nutritional density, high sward heterogeneity). Live-weight gain was substantially lower for steers on the upland system compared to the lowland system (0.31 vs. 1.04 kg d−1; s.e.d. = 0.085 kg d−1; P<0.001), leading to significant differences in estimated dry matter intakes (8.0 vs. 11.1 kg DM d−1 for upland and lowland respectively; s.e.d. = 0.68 kg DM d−1; P<0.001). While emissions per unit feed intake were similar for the lowland and upland systems, CH4 emissions per unit of live-weight gain (LWG) were substantially higher when the steers grazed the poorer quality hill pasture (760 vs 214 g kg−1 LWG; s.e.d. = 133.5 g kg−1 LWG; P<0.001). Overall any effects of breed type were relatively small relative to the combined influence of pasture type and location. 相似文献
7.
Responses of plant growth rate to nitrogen supply: a comparison of relative addition and N interruption treatments 总被引:1,自引:0,他引:1
This paper investigates the effects of uptake of nitrate and the availability of internal N reserves on growth rate in times of restricted supply, and examines the extent to which the response is mediated by the different pools of N (nitrate N, organic N and total N) in the plant. Hydroponic experiments were carried out with young lettuce plants (Lactuca sativa L.) to compare responses to either an interruption in external N supply or the imposition of different relative N addition rate (RAR) treatments. The resulting relationships between whole plant relative growth rate (RGR) and N concentration varied between linear and curvilinear (or possibly bi-linear) forms depending on the treatment conditions. The relationship was curvilinear when the external N supply was interrupted, but linear when N was supplied by either RAR methods or as a supra-optimal external N supply. These differences resulted from the ability of the plant to use external sources of N more readily than their internal N reserves. These results show that when sub-optimal sources of external N were available, RGR was maintained at a rate which was dependent on the rate of nitrate uptake by the roots. Newly acquired N was channelled directly to the sites of highest demand, where it was assimilated rapidly. As a result, nitrate only tended to accumulate in plant tissues when its supply was essentially adequate. By comparison, plants forced to rely solely on their internal reserves were never able to mobilize and redistribute N between tissues quickly enough to prevent reductions in growth rate as their tissue N reserves declined. Evidence is presented to show that the rate of remobilization of N depends on the size and type of the N pools within the plant, and that changes in their rates of remobilization and/or transfer between pools are the main factors influencing the form of the relationship between RGR and N concentration. 相似文献
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9.
Summary Changes in pH around the roots of young rape plants (Brassica napus cv. emerald) were studied using a nutrient film technique that allowed part or whole of the root system to be subjected to specific nutrient treatments. The rapidity and direction of pH change was assessed by imbedding absorbing roots in a thin film of agar containing bromocresol purple. When nitrate-fed plants were deprived of all sources of nitrogen at 15 or 17 days old, the release of H ions from the roots was immediate and uniformly distributed over the root length. When nitrate was witheld from half of the root system of nitrate-fed plants, the roots deprived of nitrate immediately started to produce H ions even though the nitrate-fed half of the root system continued to supply the whole of the plant with nitrate. However, the rate of H ion production in plants partly supplied with NO3 was less than in plants completely deprived of NO3. It is suggested that malate produced in the shoots, following nitrate reduction, may be redistributed to the roots deprived of nitrate. There, HCO3 produced by the decarboxylation of the malate masks some of the expected H ion efflux. 相似文献
10.