全文获取类型
收费全文 | 6007篇 |
免费 | 648篇 |
国内免费 | 2篇 |
出版年
2022年 | 30篇 |
2021年 | 141篇 |
2020年 | 82篇 |
2019年 | 96篇 |
2018年 | 119篇 |
2017年 | 117篇 |
2016年 | 187篇 |
2015年 | 258篇 |
2014年 | 308篇 |
2013年 | 363篇 |
2012年 | 466篇 |
2011年 | 433篇 |
2010年 | 295篇 |
2009年 | 234篇 |
2008年 | 348篇 |
2007年 | 328篇 |
2006年 | 305篇 |
2005年 | 283篇 |
2004年 | 230篇 |
2003年 | 216篇 |
2002年 | 201篇 |
2001年 | 93篇 |
2000年 | 69篇 |
1999年 | 80篇 |
1998年 | 51篇 |
1997年 | 49篇 |
1996年 | 45篇 |
1995年 | 34篇 |
1994年 | 34篇 |
1993年 | 46篇 |
1992年 | 57篇 |
1991年 | 60篇 |
1990年 | 52篇 |
1989年 | 51篇 |
1988年 | 50篇 |
1987年 | 42篇 |
1986年 | 39篇 |
1985年 | 59篇 |
1984年 | 47篇 |
1983年 | 41篇 |
1982年 | 40篇 |
1981年 | 37篇 |
1980年 | 37篇 |
1979年 | 46篇 |
1978年 | 35篇 |
1977年 | 32篇 |
1976年 | 31篇 |
1974年 | 53篇 |
1973年 | 33篇 |
1972年 | 26篇 |
排序方式: 共有6657条查询结果,搜索用时 156 毫秒
1.
2.
D C Rideout M Lambert D A Kendall G R Moe D G Osterman H P Tao I B Weinstein E T Kaiser 《Journal of cellular physiology》1985,124(3):365-371
Four amphiphilic peptides, each with net charges of +2 or more at neutrality and molecular weights under 4 kilodaltons, were found to mediate the adhesion of normal rat kidney fibroblasts to polystyrene surfaces. Two of these peptides, a model for calcitonin (peptide 1, MCT) and melittin (peptide 2, MEL), form amphiphilic alpha-helical structures at aqueous/nonpolar interfaces. The other two, a luteinizing hormone-releasing hormone model (peptide 3, LHM) and a platelet factor model (peptide 4, MPF) form beta-strand structures in amphiphilic environments. Although it contains only 10 residues, LHM mediated adhesion to surfaces coated with solutions containing as little as 10 pmoles/ml of peptide. All four of these peptides were capable of forming monolayers at air-buffer interfaces with collapse pressures greater than 20 dynes/cm. None of these four peptides contains the tetrapeptide sequence Arg-Gly-Asp-Ser, which has been associated with fibronectin-mediated cell adhesion. Ten polypeptides that also lacked the sequence Arg-Gly-Asp-Ser but were nonamphiphilic and/or had net charges less than +2 at neutrality were all incapable of mediating cell adhesion (Pierschbacher and Ruoslahti, 1984). The morphologies of NRK cells spread on polystyrene coated with peptide LHM resemble the morphologies on fibronectin-coated surfaces, whereas cells spread on surfaces coated with MCT or MEL exhibit strikingly different morphologies. The adhesiveness of MCT, MEL, LHM, and MPF implies that many amphiphilic cationic peptides could prove useful as well defined adhesive substrata for cell culture and for studies of the mechanism of cell adhesion. 相似文献
3.
Sabrina Nickel Farnusch Kaschani Tom Colby Renier A.L. van der Hoorn Markus Kaiser 《Bioorganic & medicinal chemistry》2012,20(2):601-606
Activity-based protein profiling represents a powerful methodology to probe the activity state of enzymes under various physiological conditions. Here we present the development of a para-nitrophenol phosphonate activity-based probe with structural similarities to the potent agrochemical paraoxon. We demonstrate that this probes labels distinct serine hydrolases with the carboxylesterase CXE12 as the predominant target in Arabidopsis thaliana. The designed probe features a distinct labeling pattern and therefore represents a promising chemical tool to investigate physiological roles of selected serine hydrolases such as CXE12 in plant biology. 相似文献
4.
Amanda McGrosky Carlo Meloro Ana Navarrete Sandra A. Heldstab Andrew C. Kitchener Karin Isler Marcus Clauss 《American journal of primatology》2019,81(8)
Although it is generally assumed that among mammals and within mammal groups, those species that rely on diets consisting of greater amounts of plant fiber have larger gastrointestinal tracts (GIT), statistical evidence for this simple claim is largely lacking. We compiled a dataset on the length of the small intestine, caecum, and colon in 42 strepsirrhine, platyrrhine, and catarrhine primate species, using specimens with known body mass (BM). We tested the scaling of intestine length with BM, and whether dietary proxies (percentage of leaves and a diet quality index) were significant covariates in these scaling relationships, using two sets of models: one that did not account for the phylogenetic structure of the data, and one that did. Intestine length mainly scaled geometrically at exponents that included 0.33 in the confidence interval; Strepsirrhini exhibited particularly long caeca, while those of Catarrhini were comparatively short. Diet proxies were only significant for the colon and the total large intestine (but not for the small intestine or the caecum), and only in conventional statistics (but not when accounting for phylogeny), indicating the pattern occurred across but not within clades. Compared to terrestrial Carnivora, primates have similar small intestine lengths, but longer large intestines. The data on intestine lengths presented here corroborate recent results on GIT complexity, suggesting that diet, as currently described, does not exhaustively explain GIT anatomy within primate clades. 相似文献
5.
Felix Roosen-Runge Marcus Hennig Tilo Seydel Fajun Zhang Maximilian W.A. Skoda Stefan Zorn Robert M.J. Jacobs Marco Maccarini Peter Fouquet Frank Schreiber 《Biochimica et Biophysica Acta - Proteins and Proteomics》2010,1804(1):68-75
We report on a combined cold neutron backscattering and spin-echo study of the short-range and long-range nanosecond diffusion of the model globular protein bovine serum albumin (BSA) in aqueous solution as a function of protein concentration and NaCl salt concentration. Complementary small angle X-ray scattering data are used to obtain information on the correlations of the proteins in solution. Particular emphasis is put on the effect of crowding, i.e. conditions under which the proteins cannot be considered as objects independent of each other. We thus address the question at which concentration this crowding starts to influence the static and in particular also the dynamical behaviour. We also briefly discuss qualitatively which charge effects, i.e. effects due to the interplay of charged molecules in an electrolyte solution, may be anticipated. Both the issue of crowding as well as that of charge effects are particularly relevant for proteins and their function under physiological conditions, where the protein volume fraction can be up to approximately 40% and salt ions are ubiquitous. The interpretation of the data is put in the context of existing studies on related systems and of existing theoretical models. 相似文献
6.
M. I. Jsselstijn J. Kaiser F. L. van Delft H. E. Schoemaker F. P. J. T. Rutjes 《Amino acids》2003,24(3):263-266
Summary. Novel synthetic procedures for the modification of non-proteinogenic acetylene-containing amino acids have been developed.
The functionalization either proceeds via zinc/copper-mediated introduction of alkyl substituents, or via tungsten-catalyzed
ring-closing alkyne metathesis reactions.
Received March 28, 2002 Accepted October 3, 2002 Published online December 18, 2002
Acknowledgements These investigations are supported (in part) by the Netherlands Research Council for Chemical Sciences (CW) with financial
aid from the Netherlands Technology Foundation (STW).
Authors' address: Floris P. J. T. Rutjes, Prof. Dr., Department of Organic Chemistry, University of Nijmegen, Toernooiveld 1, NL-6525 ED Nijmegen,
The Netherlands, E-mail: rutjes@sci.kun.nl
2, selected data: 1H NMR (300 MHz, CDCl3) δ 5.32 (d, J = 7.7 Hz, 1H), 4.44–4.40 (m, 1H), 3.76 (s, 3H), 2.75–2.73 (d, J = 5.0 Hz, 2H), 1.44 (s, 9H); 13C NMR (75 MHz, CDCl3) δ 171.0, 155.0, 80.3, 74.6, 52.6, 51.9, 41.7, 28.3, 24.0; mp = 55°C.
Typical procedure for 5: zinc dust (116 mg, 1.408 mmol) was weighed into a 20 mL flask, which was repeatedly evacuated (with heating using a heat
gun) and flushed with argon. Dry DMF (0.5 mL, distilled from CaH2) and 1,2-dibromoethane (9.2 μL, 0.106 mmol) were added and the flask was heated at 80°C for 40 min. The reaction mixture was allowed to cool to room temperature,
trimethylsilyl chloride (4 μL, 0.035 mmol) was added and the resulting mixture was stirred vigorously for a further 30 min under argon. Iodocyclohexane
(69 μl, 0.528 mmol) was added and stirred at room temperature for 3 h more after which stirring was ceased to settle the zinc.
CuCN (41 mg, 0.458 mmol) and LiCl (40 mg, 0.915 mmol) were heated to 150°C for 2 h and cooled to room temperature. Addition
of DMF (1 mL) formed a soluble CuCN·2LiCl complex within 5 min. After cooling the Cu-complex to −15°C, the organozinc reagent
was added dropwise followed by the bromoacetylene 2 (116 mg, 0.352 mmol). The mixture was allowed to stir overnight at room temperature. Water was added and the suspension was
extracted using heptane, washed with brine, dried (MgSO4) and concentrated. Purification using flash column chromatography (10% EtOAc in heptane) yielded 5 (100 mg, 81%) as a colorless oil. 5: IR ν 3355, 2929, 2852, 2359, 2337, 1749, 1717, 1498, 1447, 1365, 1251, 1181, 1060; 1H NMR (300 MHz, CDCl3) δ 5.28 (d, J = 7.7 Hz, 1H), 4.43–4.38 (m, 1H), 3.73 (s, 3H), 2.69–2.63 (m, 2H), 2.13 (m, 1H), 1.73–1.22 (m, 10H), 1.43 (s, 9H); 13C NMR (75 MHz, CDCl3) δ 171.4, 155.0, 88.1, 79.9, 73.8, 52.3, 32.7, 32.7, 28.8, 28.2, 25.8, 24.6, 23.1; HRMS (EI): calculated for C17H27NO4 309.1940, found 309.1937.
A solution of the tungsten catalyst (7 mg, 10 mol%) in C6H5Cl (2 mL) was treated with a solution of 14 (49.0 mg, 0.120 mmol) in C6H5Cl (5.0 mL) under an argon atmosphere and the resulting mixture was heated at 80°C for 3 h. Evaporation followed by flash
column chromatography (80% EtOAc in heptane) afforded 15 (21.0 mg, 50%; 64% after correction for starting material) and 14 (16 mg, 33%) as colorless oils. 15: [α]D =–14.6 (c = 1, CH2Cl2); IR ν 3313, 2931, 2865, 2249, 1744, 1667, 1520, 1366, 1170; 1H NMR (400 MHz, CDCl3) δ 7.14 (d, J = 8.7 Hz, 1H), 6.08 (d, J = 8.3 Hz, 1H), 4.78 (q, J = 6.8 Hz, 1H), 4.27 (q, J = 7.9 Hz, 1H), 3.73 (s, 3H), 2.17–2.15 (m, 4H), 2.07–1.96 (m, 2H), 1.79–1.52 (m, 4H), 1.45 (s, 9H), 0.89–0.83 (m, 2H); 13C NMR (100 MHz, CDCl3) δ 173.2, 171.8, 155.8, 80.4, 80.2, 79.3, 53.8, 52.5, 51.2, 32.8 (2×), 28.1, 24.6, 24.2, 18.3 (2×); HRMS (EI): calculated for
C18H28N2O5 相似文献
7.
Nanoporous alumina membranes were silanized with aminopropylsilane and iminodiacetic acid (IDA) groups were generated in situ by reaction with iodoacetate. The membranes were mounted in standard filter holders, connected to a HPLC system and saturated with selected metal ions. Cu(II) allowed the capture of chicken muscle lactate dehydrogenase with such stability, repeatability and reproducibility that Michaelis–Menten kinetics could be studied. The IDA surface was stable for months and could be depleted and regenerated with metal ions multiple times without appreciable loss of capacity. The binding of lactate dehydrogenase influenced the backpressure to the extent that could be expected for a monolayer according to Poiseuilles law. 相似文献
8.
The eicosanoids in biology and medicine 总被引:2,自引:0,他引:2
A J Marcus 《Journal of lipid research》1984,25(13):1511-1516
9.
10.
Synthesis of human walking: A planar model for single support 总被引:3,自引:0,他引:3
A mathematical model for the single support phase of normal, level, human walking is formulated. The motion of the lower extremity is synthesized using a preprogrammed set of inputs, recognized by the model as a simple collection of applied joint moments.
Two mechanisms are forwarded as candidates for producing the observed peaks in the vertical ground reaction. The first, stance knee flexion-extension, generates the necessary level of whole-body vertical acceleration during the initial region of single support (opposite toe-off to heel-off). A model accounting for the determinants of foot and knee interaction then predicts the second peak to be the result of an increasing ankle moment in the region from heel-off to opposite heel-strike. 相似文献