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Decru Eva Vranken Nathan Maetens Heleen Mertens De Vry Amber Kayenbergh Annelies Snoeks Jos Van Steenberge Maarten 《Hydrobiologia》2022,849(8):1743-1762
Hydrobiologia - We present an extensive DNA barcoding study of the fish species of the Lake Edward system, including information on intraspecific variation. The DNA barcode gene, cytochrome c... 相似文献
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Evolutionarily conserved role of nucleostemin: controlling proliferation of stem/progenitor cells during early vertebrate development 下载免费PDF全文
Beekman C Nichane M De Clercq S Maetens M Floss T Wurst W Bellefroid E Marine JC 《Molecular and cellular biology》2006,26(24):9291-9301
Nucleostemin (NS) is a putative GTPase expressed preferentially in the nucleoli of neuronal and embryonic stem cells and several cancer cell lines. Transfection and knockdown studies indicated that NS controls the proliferation of these cells by interacting with the p53 tumor suppressor protein and regulating its activity. To assess the physiological role of NS in vivo, we generated a mutant mouse line with a specific gene trap event that inactivates the NS allele. The corresponding NS(-/-) embryos died around embryonic day 4. Analyses of NS mutant blastocysts indicated that NS is not required to maintain pluripotency, nucleolar integrity, or survival of the embryonic stem cells. However, the homozygous mutant blastocysts failed to enter S phase even in the absence of functional p53. Haploid insufficiency of NS in mouse embryonic fibroblasts leads to decreased cell proliferation. NS also functions in early amphibian development to control cell proliferation of neural progenitor cells. Our results show that NS has a unique ability, derived from an ancestral function, to control the proliferation rate of stem/progenitor cells in vivo independently of p53. 相似文献
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Driessens G Nuttin L Gras A Maetens J Mievis S Schoore M Velu T Tenenbaum L Préat V Bruyns C 《Cancer immunology, immunotherapy : CII》2011,60(2):273-281
Vaccination of dendritic cells (DC) combined with GM-CSF secreting tumor cells has shown good therapeutic efficacy in several
tumor models. Nevertheless, the engineering of GM-CSF secreting tumor cell line could represent a tedious step limiting its
application for treatment in patients. We therefore developed in rats, an “all in vivo” strategy of combined vaccination using
an in vivo local irradiation of the tumor as a source of tumor antigens for DC vaccines and an exogenous source of GM-CSF.
We report here that supplying recombinant mGM-CSF by local injections or surgical implantation of osmotic pumps did not allow
reproducing the therapeutic efficacy observed with in vitro prepared combined vaccines. To bypass this limitation possibly
due to the short half-life of recombinant GM-CSF, we have generated adeno-associated virus coding for mGM-CSF and tested their
efficacy to transduce tumor cells in vitro and in vivo. The in vivo vaccines combining local irradiation and AAV2/1-mGM-CSF
vectors showed high therapeutic efficacy allowing to cure 60% of the rats with pre-implanted tumors, as previously observed
with in vitro prepared vaccines. Same efficacy has been observed with a second generation of vaccines combining DC, local
tumor irradiation, and the controlled supply of recombinant mGM-CSF in poloxamer 407, a biocompatible thermoreversible hydrogel.
By generating a successful “all in vivo” vaccination protocol combining tumor radiotherapy with DC vaccines and a straightforward
supply of GM-CSF, we have developed a therapeutic strategy easily translatable to clinic that could become accessible to a
much bigger number of cancer patients. 相似文献
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Efficient mouse transgenesis using Gateway-compatible ROSA26 locus targeting vectors and F1 hybrid ES cells 下载免费PDF全文
Omar Nyabi Michael Naessens Katharina Haigh Agnieszka Gembarska Steven Goossens Marion Maetens Sarah De Clercq Benjamin Drogat Lieven Haenebalcke Sonia Bartunkova Ilse De Vos Bram De Craene Mansour Karimi Geert Berx Andras Nagy Pierre Hilson Jean-Christophe Marine Jody J. Haigh 《Nucleic acids research》2009,37(7):e55
The ability to rapidly and efficiently generate reliable Cre/loxP conditional transgenic mice would greatly complement global high-throughput gene targeting initiatives aimed at identifying gene function in the mouse. We report here the generation of Cre/loxP conditional ROSA26-targeted ES cells within 3–4 weeks by using Gateway® cloning to build the target vectors. The cDNA of the gene of interest can be expressed either directly by the ROSA26 promoter providing a moderate level of expression or by a CAGG promoter placed in the ROSA26 locus providing higher transgene expression. Utilization of F1 hybrid ES cells with exceptional developmental potential allows the production of germ line transmitting, fully or highly ES cell-derived mice by aggregation of cells with diploid embryos. The presented streamlined procedures accelerate the examination of phenotypical consequences of transgene expression. It also provides a unique tool for comparing the biological activity of polymorphic or splice variants of a gene, or products of different genes functioning in the same or parallel pathways in an overlapping manner. 相似文献
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