全文获取类型
收费全文 | 2228篇 |
免费 | 242篇 |
出版年
2023年 | 17篇 |
2022年 | 9篇 |
2021年 | 68篇 |
2020年 | 40篇 |
2019年 | 47篇 |
2018年 | 69篇 |
2017年 | 48篇 |
2016年 | 74篇 |
2015年 | 139篇 |
2014年 | 135篇 |
2013年 | 136篇 |
2012年 | 186篇 |
2011年 | 147篇 |
2010年 | 112篇 |
2009年 | 86篇 |
2008年 | 136篇 |
2007年 | 113篇 |
2006年 | 111篇 |
2005年 | 84篇 |
2004年 | 82篇 |
2003年 | 76篇 |
2002年 | 84篇 |
2001年 | 37篇 |
2000年 | 34篇 |
1999年 | 46篇 |
1998年 | 18篇 |
1997年 | 14篇 |
1996年 | 20篇 |
1995年 | 8篇 |
1994年 | 13篇 |
1993年 | 10篇 |
1992年 | 27篇 |
1991年 | 23篇 |
1990年 | 14篇 |
1989年 | 16篇 |
1988年 | 15篇 |
1987年 | 19篇 |
1986年 | 15篇 |
1985年 | 17篇 |
1984年 | 15篇 |
1983年 | 10篇 |
1982年 | 10篇 |
1981年 | 8篇 |
1979年 | 13篇 |
1978年 | 8篇 |
1974年 | 4篇 |
1973年 | 9篇 |
1971年 | 9篇 |
1970年 | 8篇 |
1968年 | 7篇 |
排序方式: 共有2470条查询结果,搜索用时 116 毫秒
1.
2.
3.
M. Filek J. Biesaga-Kościelniak I. Marcińska M. Cvikrová I. Macháčková J. Krekule 《Biologia Plantarum》2010,54(3):483-487
The contents of endogenous free and conjugated polyamines, putrescine (Put) and spermidine (Spd), were determined during 9
week of vernalization (at 5 °C) in winter wheat seedlings cultivated on Murashige and Skoog media without (MS0) and with 2
mg dm−3 zearalenone (MSZEN). At the 4th week of chilling treatment, which is sufficient to induce generative development in 30 % of plants, the marked increase in
free and conjugated forms of Put and free Spd were observed. The presence of ZEN in medium significantly accelerated the vernalization.
About 20 % of plants treated with ZEN flowered already after 2 weeks and 40 % after 3 weeks of chilling. Significantly higher
content of free Put was determined in roots grown on MSZEN compared with MS0 during the first 5 weeks of vernalization with
maximum at the 4th week. After germination, a marked decrease in free Spd content was observed both in plants grown on MS0 and MSZEN. Application
of ZEN significantly slowed down the Spd decline in leaves and roots during the first and second week of vernalization. The
content of Spd and its conjugates decreased in vernalized plants after 1 week of cultivation at 20 °C. 相似文献
4.
C Berche J P Mach J D Lumbroso C Langlais F Aubry F Buchegger S Carrel P Rougier C Parmentier M Tubiana 《BMJ (Clinical research ed.)》1982,285(6353):1447-1451
Transaxial tomoscintigraphy (or single-photon emission computerised tomography) was used to detect secondary deposits of carcinoma in 17 patients who had been injected with iodine-131-labelled monoclonal antibodies against carcinoembryonic antigen. Of 17 tumor sites studied by tomoscintigraphy 16 were detected (sensitivity 94%); five sites had a volume smaller than 10 cm3. Tomoscintigraphy also detected three unknown tumour deposits later confirmed by surgery or radiology. In contrast, when 21 tumour sites in the same patients were studied by rectilinear scintigraphy, only nine tumour sites were detected (sensitivity 43%), of which eight had a volume larger than 50 cm3. 相似文献
5.
DnaB proteolysis in vivo regulates oligomerization and its localization at oriC in Bacillus subtilis
William H. Grainger Cristina Machón David J. Scott Panos Soultanas 《Nucleic acids research》2010,38(9):2851-2864
Initiation of bacterial DNA replication at oriC is mediated by primosomal proteins that act cooperatively to melt an AT-rich region where the replicative helicase is loaded prior to the assembly of the replication fork. In Bacillus subtilis, the dnaD, dnaB and dnaI genes are essential for initiation of DNA replication. We established that their mRNAs are maintained in fast growing asynchronous cultures. DnaB is truncated at its C-terminus in a growth phase-dependent manner. Proteolysis is confined to cytosolic, not to membrane-associated DnaB, and affects oligomerization. Truncated DnaB is depleted at the oriC relative to the native protein. We propose that DNA-induced oligomerization is essential for its action at oriC and proteolysis regulates its localization at oriC. We show that DnaB has two separate ssDNA-binding sites one located within residues 1–300 and another between residues 365–428, and a dsDNA-binding site within residues 365–428. Tetramerization of DnaB is mediated within residues 1–300, and DNA-dependent oligomerization within residues 365–428. Finally, we show that association of DnaB with the oriC is asymmetric and extensive. It encompasses an area from the middle of dnaA to the end of yaaA that includes the AT-rich region melted during the initiation stage of DNA replication. 相似文献
6.
7.
DNA typing of HLA-DR
β
chain genes can discriminate between undetected alleles and real homozygotes 总被引:1,自引:0,他引:1
The polymorphism of HLA-DR antigens has been studied by Southern blot hybridization under conditions specific for the detection of the DR
chain genes. Haplotype-specific patterns were defined with DNA from DR1, 2, 3, 4, 7, w8, w11, w12, and W13 homozygous typing cells, with restriction enzymes Eco RI, Bgl I, and Pvu II. Certain serological specificities, such as DR2, DR3, and DR7, can be encoded by distinct allelic forms of DR
chain genes. The procedure of DNA typing was applied to family analysis of individuals expressing only a single DR specificity upon serological typing. Three cases are described here: (1) in family GR, phenotypic DR 7 homozygotes correspond to genomic heterozygotes, and a novel DR7 allele is described: (2) in family RU, the genes corresponding to a serologically undetected (blank) DR allele were identified by restriction fragment length polymorphism (RFLP); this novel DR haplotype has an RFLP pattern similar to those of the DRw52 family, even though this specificity was not expressed on the DR-blank lymphocytes; (3) in family RG, there is no blank allele, but a homozygote RFLP situation at the DR subregion. 相似文献
8.
9.
10.
Physiological studies on cAMP synthesis in Bacillus subtilis 总被引:1,自引:0,他引:1
Abstract cAMP was detected in Bacillus subtilis SB 19 and in a temperature sensitive mutant ts 33-6 under oxygen limitation. Growth rate and cAMP content were negatively correlated. Dinitrophenol and α-methylglucoside elicited an increase of the synthesis of cAMP and an increase of the intracellular cAMP content. In response to a decrease was increased. This increase of cAMP concentration did not occur if nitrate was present in the growth medium as an alternative terminal electron acceptor. 相似文献