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Hameed Asif Shahina Mariyam Young Li-Sen Lai Wei-An Sridhar Kandikere Ramaiah Young Chiu-Chung 《Plant and Soil》2019,443(1-2):73-86
Plant and Soil - To screen plant-associated Burkholderia strains for plant probiotic traits including allelochemical metabolism and understand their role on rice allelopathy using a... 相似文献
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Hameed A Shahina M Lin SY Sridhar KR Young LS Lee MR Chen WM Chou JH Young CC 《FEMS microbiology letters》2012,333(1):37-45
A strictly aerobic, Gram-negative, rod-shaped bacterium (strain CC-SAMT-1(T)) showing gliding motility was isolated from coastal seawater of China Sea, Taiwan. Strain CC-SAMT-1(T) synthesizes all-trans-zeaxanthin (6.5 ± 0.5 mg g(-1) dry biomass) as a predominant xanthophyll carotenoid. As determined by 16S rRNA gene analysis, strain CC-SAMT-1(T) shared very high sequence similarity to the members of the genera Mariniflexile (96.1-95.3%) and Gaetbulibacter (96.0-95.9%); however, it formed a distinct phyletic lineage distantly associated with Mariniflexile species. Polar lipid profile constitutes phosphatidylethanolamine, four unidentified aminolipids, four unidentified lipids, and an unidentified glycolipid. Strain CC-SAMT-1(T) contains excessive unidentified aminolipid lipid (AL2-4) and glycolipid contents, and therefore clearly distinct from Mariniflexile species. Major fatty acids (> 5% of total fatty acids) were iso-C(15:0) (14.8%), iso-C(17:0) 3-OH (11.8%), iso-C(15:1) G (10.6%), anteiso-C(15:0) (9.7%), C(16:0) (8.1%), iso-C(16:0) 3-OH (7.9%), iso-C(15:0) 3-OH (7.5%), and summed feature 3 (containing C(16:1) ω6c and/or C(16:1) ω7c) (7.5%). Menaquinone-6 (MK-6) was major respiratory quinone. DNA G+C content was 33.7 mol%. Based on polyphasic taxonomy, strain CC-SAMT-1(T) represents a novel genus and species in the family Flavobacteriaceae for which the name Siansivirga zeaxanthinifaciens gen. nov., sp. nov. is proposed. The type strain is CC-SAMT-1(T) (= BCRC 80315(T) = JCM 17682(T)). 相似文献
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Young LS Harrison BR Narayana Murthy UM Moffatt BA Gilroy S Masson PH 《Plant physiology》2006,142(2):564-573
Adenosine kinase (ADK) is a key enzyme that regulates intra- and extracellular levels of adenosine, thereby modulating methyltransferase reactions, production of polyamines and secondary compounds, and cell signaling in animals. Unfortunately, little is known about ADK's contribution to the regulation of plant growth and development. Here, we show that ADK is a modulator of root cap morphogenesis and gravitropism. Upon gravistimulation, soluble ADK levels and activity increase in the root tip. Mutation in one of two Arabidopsis (Arabidopsis thaliana) ADK genes, ADK1, results in cap morphogenesis defects, along with alterations in root sensitivity to gravistimulation and slower kinetics of root gravitropic curvature. The kinetics defect can be partially rescued by adding spermine to the growth medium, whereas the defects in cap morphogenesis and gravitropic sensitivity cannot. The root morphogenesis and gravitropism defects of adk1-1 are accompanied by altered expression of the PIN3 auxin efflux facilitator in the cap and decreased expression of the auxin-responsive DR5-GUS reporter. Furthermore, PIN3 fails to relocalize to the bottom membrane of statocytes upon gravistimulation. Consequently, adk1-1 roots cannot develop a lateral auxin gradient across the cap, necessary for the curvature response. Interestingly, adk1-1 does not affect gravity-induced cytoplasmic alkalinization of the root statocytes, suggesting either that ADK1 functions between cytoplasmic alkalinization and PIN3 relocalization in a linear pathway or that the pH and PIN3-relocalization responses to gravistimulation belong to distinct branches of the pathway. Our data are consistent with a role for ADK and the S-adenosyl-L-methionine pathway in the control of root gravitropism and cap morphogenesis. 相似文献
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Fo-Ting Shen Li-Sen Young Ming-Fang Hsieh Shih-Yao Lin Chiu-Chung Young 《Systematic and applied microbiology》2010
The alkB gene encodes for alkane 1-monooxygenase, which is a key enzyme responsible for the initial oxidation of inactivated alkanes. This functional gene can be used as a marker to assess the catabolic potential of bacteria in bioremediation. In the present study, a pair of primers was designed based on the conserved regions of the AlkB amino acid sequences of Actinobacteria, for amplifying the alkB gene from the genus Gordonia (20 Gordonia strains representing 13 species). The amplified alkB genes were then sequenced and analyzed. In the phylogenetic tree based on the translated AlkB amino acid sequences, all the Gordonia segregated clearly from other closely related genera. The sequence identity of the alkB gene in Gordonia ranged from 58.8% to 99.1%, which showed higher sequence variation at the inter-species level compared with other molecular markers, such as the 16S rRNA gene (93.1–99.8%), gyrB gene (77.5–97.3%) or catA gene (72.4–99.5%). The genetic diversity of four selected loci also showed that the alkB gene might have evolved faster than rrn operons, as well as the gyrB or catA genes, in Gordonia. All the available actinobacterial alkB gene sequences derived from the whole genome shotgun sequencing projects are phylogenetically characterized here for the first time, and they exclude the possibility of horizontal gene transfer of the alkB gene in these bacterial groups. 相似文献
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Phanit Nakayan Asif Hameed Satnam Singh Li-Sen Young Mei-Hua Hung Chiu-Chung Young 《Plant and Soil》2013,373(1-2):301-315
Aim
Phosphate-solubilizing yeasts have been under-exploited in eco-friendly maize cultivation. In this regard, soil yeasts Meyerozyma guilliermondii CC1, Rhodotorula mucilaginosa CC2 and M. caribbica CC3 were investigated for their plant growth-promoting (PGP) activities.Methods
Soil yeasts were isolated and characterized. Maize (Zea mays L. cv. Tainong No.1) and Chinese cabbage (Brassica rapa L. cv. Pekinensis) were used for seed bioassay. Growth-promoting effects of yeasts under greenhouse conditions were evaluated using maize and lettuce (Lactuca sativa L. cvs. Capitata and Taiwan sword leaf). Ultimately, M. guilliermondii CC1 was tested on field-grown maize; treatments included full-dose chemical fertilizers (CF), yeast (CC1), half-dose chemical fertilizers (½CF), CC1?+?½CF and control. Nutrient uptake, growth, and yield of maize and rhizospheric soil microbes were estimated.Results
Strain M. guilliermondii CC1 exhibited better seed vigor index in maize and Chinese cabbage. CC1?+?½CF significantly improved the dry-weights, and nutrient uptakes of maize and sword leaf lettuce under greenhouse conditions. In field, CC1?+?½CF application exerted a pronounced effect on growth of maize, cob yield, nutrient-uptake and rhizospheric soil microbial counts.Conclusion
Our results validated superior biochemical potency and PGP traits of M. guilliermondii CC1 that reduced requisite chemical fertilizer application without affecting the optimal productivity in maize. 相似文献8.
Hong-Tian Zhang Jie Luo Li-Sen Sui Xu Ma Zhong-Jie Yan Jian-Hao Lin Yu-Sheng Wang Yi-Zhao Chen Xiao-Dan Jiang Ru-Xiang Xu 《Cellular and molecular neurobiology》2009,29(8):1283-1292
Controversies exist concerning the need for mesenchymal stromal cells (MSCs) to be transdifferentiated prior to their transplantation.
In the present study, we compared the results of grafting into the rat contused spinal cord undifferentiated, adipose tissue-derived
stromal cells (uADSCs) versus ADSCs induced by two different protocols to form differentiated nervous tissue. Using Basso,
Beattie, and Bresnahan scores and grid tests, we found that three cell-treated groups, including uADSCs-treated, dADSCs induced
by Protocol 1 (dADSC-P1)-treated, and dADSCs induced by Protocol 2 (dADSC-P2)-treated groups, significantly improved locomotor
functional recovery in SCI rats, compared with the saline-treated group. Furthermore, functional recovery was better in the
uADSC-treated and dADSC-P2-treated groups than in the dADSC-P1-treated group at week 12 postinjury (P < 0.05 for dADSC-P1 group vs. uADSCs or dADSC-P2 groups). Although both protocols could induce high percentages of cells
expressing neural markers in vitro, few BrdU-labeled cells survived at the injury sites in the three cell-treated groups,
and only a small percentage of BrdU-positive cells expressed neural markers. On the other hand, the number of NF200-positive
axons in the uADSC-treated and dADSC-P2-treated groups was significantly larger than those in the dADSC-P1-treated and saline-treated
control groups. Our results indicate that ADSCs are able to differentiate into neural-like cells in vitro and in vivo. However, neural differentiated ADSCs did not result in better functional recovery than undifferentiated ones, following SCI.
In vitro neural transdifferentiation of ADSCs might therefore not be a necessary pretransplantation step. Furthermore, cellular
replacement or integration might not contribute to the functional recovery of the injured spinal cord. 相似文献
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Gravity signal transduction in primary roots 总被引:8,自引:0,他引:8
Perrin RM Young LS Murthy U M N Harrison BR Wang Y Will JL Masson PH 《Annals of botany》2005,96(5):737-743
AIMS: The molecular mechanisms that correlate with gravity perception and signal transduction in the tip of angiosperm primary roots are discussed. SCOPE: Gravity provides a cue for downward orientation of plant roots, allowing anchorage of the plant and uptake of the water and nutrients needed for growth and development. Root gravitropism involves a succession of physiological steps: gravity perception and signal transduction (mainly mediated by the columella cells of the root cap); signal transmission to the elongation zone; and curvature response. Interesting new insights into gravity perception and signal transduction within the root tip have accumulated recently by use of a wide range of experimental approaches in physiology, biochemistry, genetics, genomics, proteomics and cell biology. The data suggest a network of signal transduction pathways leading to a lateral redistribution of auxin across the root cap and a possible involvement of cytokinin in initial phases of gravicurvature. CONCLUSION: These new discoveries illustrate the complexity of a highly redundant gravity-signalling process in roots, and help to elucidate the global mechanisms that govern auxin transport and morphogenetic regulation in roots. 相似文献
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Li-Sen Wang Xiang-Yang Ge Wei-Guo Zhang 《World journal of microbiology & biotechnology》2007,23(4):461-465
A novel raw-starch-digesting glucoamylase producer, Rhizopus sp. W-08, and Saccharomyces cerevisiae Z-06 were used in a fed batch process for simultaneous saccharification and fermentation of raw corn flour. Ethanol concentration
of 21% (v/v) was obtained after 48 h. The conversion efficiency of raw corn flour to ethanol was 94.5% of the theoretical
ethanol yield. 相似文献