排序方式: 共有15条查询结果,搜索用时 15 毫秒
1.
T. A. Larkina A. L. Sazanova K. A. Fomichev O. Y. Barkova A. A. Sazanov T. Malewski K. Jaszczak 《Russian Journal of Genetics》2011,47(8):1012-1015
The quantitative traits of mass and percentage of abdominal fat in chicken and various types of obesity in mammals are homologous
and functionally similar. Therefore, the genes involved in obesity development in humans and laboratory rodents as well as
those responsible for pig lard thickness could be involved in abdominal fat deposition in broilers. Expression of candidate
genes FABP1, FABP2, FABP3, HMGA1, MC4R, PPARG, PPARGC1A, POMC and PTPN1 was studied in fat, liver, colon, muscle, pituitary gland, and brain in chicken (broilers) using real-time PCR. Significant
difference in the HMGA1 gene expression in the liver of broiler chicken with high (3.5 ± 0.18%) and low (1.9 ± 0.56%) abdominal fat concentration
has been revealed. The expression of this gene was been shown to correlate with the amount (0.7, P ≤ 0.01) and mass (0.7, P ≤ 0.01) of abdominal fat. The PPARG gene expression in liver in the same chicken subsets was also significantly different. Correlation coefficients of the gene
expression with the abdominal fat amount and mass were respectively 0.55 (P ≤ 0.05) and 0.57 (P ≤ 0.01). Based on these results, we suggest that the HMGA1 and PPARG genes are involved in abdominal fat deposition. The search for single nucleotide polymorphisms (SNPs) in the HMGA and PPARG regulatory regions could facilitate identifying genetic markers for broiler breeding according to the mass and percentage
of abdominal fat. 相似文献
2.
V. V. Kolpakov E. A. Tomilova T. V. Bespalova N. Yu. Larkina M. V. Stolbov A. A. Tkachuk 《Human physiology》2016,42(2):203-213
The concept of typological variability of physiological individuality was a methodological basis for chrono-physiological assessment of habitual physical activity (HPA) and heart rate (HR) in individuals with different functional types of constitution (FTC) (low HPA (FTC-1), medium HPA (FTC-2), and high HPA (FTC-3)) living at different latitudes of West Siberia (Tyumen, 57°09’N; Khanty–Mansi Autonomous Okrug (KhMAO)–Yugra, 61°00’N; and Yamal–Nenets Autonomous Okrug (YaNAO), 63°12’N). The typical chronostructure of the circadian rhythm of HPA and HR was established for each FTC under the conditions of both mid-latitude (Tyumen) and northern regions. The regional differences were determined too. Compared to the mid-latitude parameters, the chrono-physiological assessment of typological variability of HPA and HR in FTC-1, FTC-2 and FTC-3 subjects living under the climatic and geographical conditions of KhMAO–Yugra and YaNAO in each respective group demonstrated a decrease in the midline-estimating statistic of rhythm (MESOR) and energy intensity of the circadian rhythm, an increase in the proportion of ultradian rhythms, differences in the time of HPA and HR acrophases, a decrease in synchronization and coherence indices, as well as a higher coefficient of the number of heart beats per locomotion and a lower circadian index. All of the above parameters of northerners with each FTC, with regard for the general principles of modern approaches, are not pathologies but rather regional characteristics that should be taken into consideration in the assessment of general state of health and prenosological diagnostics; they were the basis for using chronoprevention and time correction of reduced physical activity. 相似文献
3.
Laboratory mutator strain of Drosophila melanogaster is characterized by increased (up to 10(-3)-10(-4) frequency of spontaneous mutability. Mutations appear in premeiotic stages of gametes development. The majority of mutations were unstable (high frequencies of reversions, appearance of new mutations at the same and other loci, replicating instability). Localization of mobile elements mdg1, mdg2, mdg3, mdg4, copia and P element in X chromosomes of mutator individuals and its mutations y, ct, sbt was studied by hybridization in situ. In all strains P element was absent. The distribution of mdg1, mdg2, mdg3 and copia was identical in mutator strains and its derivatives, but distribution of mdg4 was different. The essential heterogeneity in localization of mdg4 and increased (up to 30-40) copy number in the mutator strain individuals was observed. The ability of single element mdg4 to autonomous transpositions was thus shown. 相似文献
4.
V. V. Kolpakov N. Yu. Larkina E. A. Tomilova A. A. Tkachuk T. V. Bespalova 《Human physiology》2017,43(4):404-415
On the basis of the concept of the typological variability of human physiological individuality, we have determined the systemic correlation between the indicators of cognitive activity and the parameters and development of emotional intelligence (EI) and the formation of typical behavior strategies in older preschool children of three functional types (FT-1, FT-2, and FT-3), including subjects with low (LHPA), medium (MHPA), and high (HHPA) levels of habitual physical activity. We revealed the EI features typical of each functional group of children and observed a strong negative correlation between the indicator of HPA and the total EI score. The typical manifestation of EI is closely related to other psychological parameters of cognitive and emotional–volitional activity (logical and imaginative thinking, state anxiety, and attention), which allowed us to establish the constitutional features of the behavior strategy on the basis of the integrated assessment of all the obtained data. In different conflict situations, children with HHPA (FT-3) made decisions more rapidly and by themselves; their decisions were active and aimed at achieving a specific goal on the basis of domination (individual problem solving). In contrast, children with LHPA (FT-1) solved the problems on the basis of the emotional state of other people and preferred to explain the situation to their parents or educators and ask for help (cooperative problem solving). Up to 10% of children with LHPA preferred not to solve the problem. 相似文献
5.
T. V. Machneva A. V. Lokhmatov I. S. Shevtsova E. A. Larkina E. P. Tkachevskaya A. F. Mironov Yu. A. Vladimirov A. N. Osipov 《Biophysics》2012,57(2):187-196
The main goal of the present study was to evaluate the comparative effectiveness of tetrapyrrol photosensitizers (protoporphyrine IX and chlorine e 6) in red (632.8 nm) and green (532.5) spectrum bands on rat blood free radical status, using the experimental model of endotoxic shock. Endotoxic shock was produced by intraperitoneal injection of lipopolysaccharide B. Irradiation effectiveness was estimated by leukocyte activation (measured with luminol-dependent chemiluminescence), superoxide dismutase activity of blood plasma (nitro blue tetrasolium assay) and lipid peroxidation (assay with cis-parinaric acid). It was found that laser irradiation has multidirectional effects on leukocyte activation, membrane lipid peroxidation and plasma SOD activity and all these effects were more pronounced in the case of endotoxic shock. Protoporphyrin was more effective in leukocyte activation and chlorine e 6 demonstrated maximal effects on blood SOD activity. 相似文献
6.
Two triad systems were synthesized from deuteroporphyrin IX by tethering 1,4-naphthoquinone derivatives and aromatic amino acids to its propionic groups using the method of mixed anhydrides. Physicochemical characteristics of the triads were studied, and the pi-electron systems of their chromophores were shown to interact. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2002, vol. 28, no. 4; see also http://www.maik.ru. 相似文献
7.
Larkina E. A. Luzgina V. N. Evstigneeva R. P. 《Russian Journal of Bioorganic Chemistry》2002,28(4):322-325
Two triad systems were synthesized from deuteroporphyrin IX by tethering 1,4-naphthoquinone derivative and aromatic amino acids to its propionic groups using the method of mixed anhydrides. Physicochemical characteristics of the triads were studied, and the -electron systems of their chromophores were shown to interact. 相似文献
8.
Simple sequence repeats (microsatellites) are found in all eukaryotic genomes. Instabilities within these sequences have been associated with several human disorders including Huntington's chorea and myotonic dystrophy. Further studies have identified links between microsatellite instability, faulty mismatch repair and certain human cancers, in particular a form of hereditary colorectal cancer. The assay system described here consists of a congenic set of yeast strains mutated in DNA replication and mismatch repair genes and assay plasmids with which it is possible to measure differences in microsatellite stability in the range of 5-850-fold. The development of this technology will allow monitoring of environmental and dietary influences on the genomic stability in the context of human disease. 相似文献
9.
Larkina TA Sazanova AL Fomichev KA Barkova OY Malewski T Jaszczak K Sazanov AA 《Journal of applied genetics》2011,52(2):225-228
The expression of nine functional candidates for QT abdominal fat weight and relative abdominal fat content was investigated by real-time polymerase chain reaction (PCR) in the liver, adipose tissue, colon, muscle, pituitary gland and brain of broilers. The high mobility group AT-hook 1 (HMG1A) gene was up-regulated in liver with a ratio of means of 2.90 (P?≤?0.01) in the «fatty» group (relative abdominal fat content 3.5?±?0.18%, abdominal fat weight 35.4?±?6.09 g) relative to the «lean» group (relative abdominal fat content 1.9?±?0.56%, abdominal fat weight 19.2?±?5.06 g). Expression of this gene was highly correlated with the relative abdominal fat content (0.70, P?≤?0.01) and abdominal fat weight (0.70, P?≤?0.01). The peroxisome proliferator-activated receptor gamma (PPARG) gene was also up-regulated in the liver with a ratio of means of 3.34 (P?≤?0.01) in the «fatty» group relative to the «lean» group. Correlation of its expression was significant with both the relative abdominal fat content (0.55, P?≤?0.05) and the abdominal fat weight (0.57, P?≤?0.01). These data suggest that the HMG1A and PPARG genes were candidate genes for abdominal fat deposition in chickens. Searching of rSNPs in regulatory regions of the HMG1A and PPARG genes could provide a tool for gene-assisted selection. 相似文献
10.
Larkina TA Sazanova AL Fomichev KA Barkova OIu Sazanova AA Malwski T Jaszczak K 《Genetika》2011,47(8):1140-1144
The quantitative traits of mass and percentage of abdominal fat in chicken and various types of obesity in mammals are homologous and functionally similar. Therefore, the genes involved in obesity development in humans and laboratory rodents as well as those responsible for pig lard thickness could be involved in abdominal fat deposition in broilers. Expression of candidate genes FABP1, FABP2, FABP3, HMGA1, MC4R, PPARG, PPARGC1A, POMC and PTPN1 was studied in fat, liver, colon, muscle, hypophysis, and brain in chicken (broilers) using real-time PCR. Significant difference in the HMGA1 gene expression in the liver of broiler chicken with high (3.5 +/- 0.18%) and low (1.9 +/- 0.56%) abdominal fat concentration has been revealed. The expression of this gene was been shown to correlate with the amount (0.7, P < or = 0.01) and mass (0.7, P < or = 0.01) of abdominal fat. The PPARG gene expression in liver in the same chicken subsets was also significantly different. Correlation coefficients of the gene expression with the abdominal fat amount and mass were respectively 0.55 (P < or = 0.05) and 0.57 (P < or = 0.01). Based on these results, we suggest that the HMGA1 and PPARG genes are involved in abdominal fat deposition. The search for single nucleotide polymorphisms (SNPs) in the HMGA and PPARG regulatory regions could facilitate identifying genetic markers for broiler breeding according to the mass and percentage of abdominal fat. 相似文献