全文获取类型
收费全文 | 616篇 |
免费 | 59篇 |
国内免费 | 196篇 |
出版年
2023年 | 5篇 |
2022年 | 10篇 |
2021年 | 24篇 |
2020年 | 13篇 |
2019年 | 22篇 |
2018年 | 13篇 |
2017年 | 14篇 |
2016年 | 21篇 |
2015年 | 29篇 |
2014年 | 29篇 |
2013年 | 31篇 |
2012年 | 63篇 |
2011年 | 59篇 |
2010年 | 49篇 |
2009年 | 46篇 |
2008年 | 48篇 |
2007年 | 39篇 |
2006年 | 35篇 |
2005年 | 34篇 |
2004年 | 24篇 |
2003年 | 19篇 |
2002年 | 30篇 |
2001年 | 27篇 |
2000年 | 16篇 |
1999年 | 16篇 |
1998年 | 15篇 |
1997年 | 6篇 |
1996年 | 6篇 |
1995年 | 5篇 |
1994年 | 11篇 |
1993年 | 4篇 |
1992年 | 10篇 |
1991年 | 10篇 |
1990年 | 3篇 |
1989年 | 11篇 |
1988年 | 6篇 |
1987年 | 6篇 |
1986年 | 3篇 |
1985年 | 6篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1982年 | 4篇 |
1981年 | 3篇 |
1980年 | 3篇 |
1979年 | 6篇 |
1976年 | 3篇 |
1973年 | 3篇 |
1972年 | 3篇 |
1957年 | 3篇 |
1956年 | 3篇 |
排序方式: 共有871条查询结果,搜索用时 203 毫秒
1.
2.
作者在广东省梅县市发现四个吸虫新种:1.鼠丽色吸虫,新种Reesella ratta sp. nov., 2.后囊蛙蠕吸虫,新种Batrachotrema optstosacca sp. nov., 3.梅县后平睾吸虫,新种Opisthioparorchis meixianensis sp. nov., 4.大卵后平睾吸虫,新种Opisthioparorchis megaloonos sp. nov.,它们分别归隶于蹲茎科Cathaemasiidae及蛙蠕科Bstrachotrematidae。模式标本保存于中山医科大学寄生虫学研究所。 相似文献
3.
Lu49888, a photoaffinity analog of verapamil, was used to identify specific binding sites for phenylalkylamines of calcium channels present in rabbit skeletal muscle microsomes. Direct binding equilibrium measurements and displacement curves of Lu49888 by its non-radioactive analog yielded an apparent single class of binding sites with Kd and Bmax values of 16.5 nM and 7.5 pmol/mg respectively. Lu49888 was specifically incorporated into three proteins of apparently 165 kDa, and 33 kDa. Incorporation into the 55-kDa protein was blocked by 10--50-fold higher concentrations of unlabeled phenylalkylamines compared to incorporation into the 165-kDa protein, suggesting that the 165-kDa and 55-kDa proteins contain a high and a low-affinity verapamil-binding site respectively. The photoaffinity-labeled proteins were solubilized by 1% digitonin or 1% Chaps in roughly equal amounts. The 165-kDa protein bound to wheat-germ-agglutinin(WGA)--Sepharose and sedimented in sucrose density gradients with the same constant as the purified dihydropyridine receptor, which has been reconstituted to a functional calcium channel. The 55-kDa membrane protein did not bind to the WGA-Sepharose column and sedimented in sucrose density gradients with a lower s value than the 165-kDa protein. The 165-kDa but not the 55-kDa membrane protein was specifically labeled by azidopine, the photoaffinity analogue of dihydropyridines. The 55-kDa protein of the purified dihydropyridine receptor was not significantly labeled by Lu49888 showing that the 55-kDa protein of the membrane is unrelated to the purified high-affinity dihydropyridine receptor. 相似文献
4.
Phosphorylation of cGMP-dependent protein kinase increases the affinity for cyclic AMP 总被引:2,自引:0,他引:2
Cyclic-GMP-dependent protein kinase contains two binding sites for cGMP, which have different affinities for cGMP. Autophosphorylation of the enzyme affects mainly the binding of cGMP to the 'high'-affinity site (site 1). The enzyme binds cAMP and cAMP stimulates the phosphotransferase activity of the native enzyme half-maximally at 44 microM. Autophosphorylation of the enzyme decreases the apparent Ka value to 7 microM. Autophosphorylation does not affect the catalytic rate of the enzyme if measured at a saturating concentration of ATP. Tritiated cAMP apparently binds at 4 degrees C to one site with a Kd value of 3 microM. Binding to the second site is not measurable. Autophosphorylation of the enzyme increases the affinity of the high-affinity site for cAMP sixfold (Kd 0.46 microM) and allows the detection of a second site. In accordance with these data the dissociation rate of [3H]cAMP from the high-affinity site is decreased from 4.5 min-1 to 1.2 min-1 by autophosphorylation. Experiments in which unlabeled cAMP competes with [3H] cGMP for the two binding sites confirmed these results. Recalculation of the competition curves by a computer program for two binding sites indicated that autophosphorylation decreases the Kd value for binding of cAMP to the high-affinity site from 1.9 microM to 0.17 microM. Autophosphorylation does not affect significantly the affinity for the second site. Kd values for site 2 varied from 17 microM to 40 microM. These results suggest that autophosphorylation of cGMP-dependent protein kinase increases the affinity of the enzyme for cAMP by affecting mainly the properties of binding site 1. 相似文献
5.
6.
对我国52种微茎类吸虫的18项成虫形态学特征进行主成分分析,结果表明:卵巢位置、子宫延伸位置等7项性状对第一主成分贡献较大,提示描述器官位置的指标是重要的分类依据。52个虫种在前三个主成分上的排序图显示应将其划分成4个亚科。 相似文献
7.
本文就萤叶甲亚科中柱萤叶甲属鞘翅具黑色刻点的种类进行研究,共记述4种,我国已记录3种,其中1种为新种。 相似文献
8.
9.
维生素C·Cu·菲咯啉系统对DNA的定位损伤 总被引:4,自引:0,他引:4
维生素C·Cu·菲咯啉系统对DNA的定位损伤柯德森,王爱国,罗广华(中国科学院华南植物研究所,广州510650)关键词活性氧;DNA;定位损伤活性氧对DNA的损伤已经被很多工作所证实”””,这种伤害是由0B”直接作用于DNA所引起的”’“。但是OH’... 相似文献
10.
本文报道在湛江市附近海域海鸟体内获得的两种吸虫,经鉴定为新种,命名为巨口类茎吸虫,新种Microphalloides macrostonrs sp.nov.,珊瑚多黄吸虫,新种Multivitellus coralius sp.nov. 相似文献