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A. A. Makarenko A. Yu. Arinbasarova T. N. Kuvichkina S. V. Balashov A. N. Reshetilov 《Applied Biochemistry and Microbiology》2005,41(5):457-459
Parameters of degradation of p-toluenesulfonate (TS) by free and agar-embedded Comamonas testosteroni BS1310 (pBS1010) cells were determined. The maximum rate of TS degradation was 25% lower in immobilized than free cells, equaling 11 nmol min?1 mg?1 cells. Degradation of TS by both free and immobilized cells was associated with molecular oxygen consumption (molar ratio 1 : 2). In a plug-flow reactor, the degradation rate was 10.4 nmol min?1 mg?1 cells. The results can be applied to designing reactors for TS degradation in sewage and developing biosensors. 相似文献
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Kitova AE Kuvichkina TN Arinbasarova AIu Reshetilov AN 《Prikladnaia biokhimiia i mikrobiologiia》2004,40(3):307-311
Degradation of 2,4-dinitrophenol (2,4-DNP) by the cells of Rhodococcus erythropolis HL PM-1 was studied. The enzymes involved in 2,4-DNP degradation were inducible, and their resynthesis took place during the process. Cell immobilization by embedding into agar gels decreased the degrader activity. Maximum rates of 2,4-DNP degradation by free and immobilized cells were 10.0 and 5.4 nmol/min per mg cells, respectively. The concentration dependence of 2,4-DNP degradation was typical of substrate inhibition kinetics. The immobilized cells were used in a model reactor designed for 2,4-DNP biodegradation. Its maximum capacity was 0.45 nmol/min per mg cells at a volumetric flow rate of 20 h-1. The reactor operated for 14 days without losing capacity; its half-lifetime equaled 16 days. 相似文献
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A. E. Kitova T. N. Kuvichkina P. V. Il'yasov A. Yu. Arinbasarova A. N. Reshetilov 《Applied Biochemistry and Microbiology》2002,38(5):500-505
A model of a reactor-type biosensor based on the Rhodococcus erythropolis HL PM-1 was developed for amperometric detection of 2,4-dinitrophenol (2,4-DNP). The effects of the matrix material (agar and calcium alginate gels, ceramic support, and cellulose powder) on the biosensor signal concentration dependence, detection time, and biosensor stability were studied. In the case of bacterial cells immobilized on cellulose powder, the lower limit of 2,4-DNP detection was 20 M and the time of single analysis, the biosensor recovery included, was 30–50 min. In the continuous detection mode, the biosensor response was maintained at a stable level without biosensor inactivation for ten days. The biosensor can be used as an element of a complex analytical system for detecting nitroaromatic compounds in samples. 相似文献
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Kitova AE Kuvichkina TN Il'iasov PV Arinbasarova AIu Reshetilov AN 《Prikladnaia biokhimiia i mikrobiologiia》2002,38(5):585-590
A model of a reactor-type biosensor based on the Rhodococcus erythropolis HL PM-1 was developed for amperometric detection of 2,4-dinitrophenol (2,4-DNP). The effects of the matrix material (agar and calcium alginate gels, ceramic support, and cellulose powder) on the biosensor signal concentration dependence, detection time, and biosensor stability were studied. In case of bacterial cells immobilized on cellulose powder, the lower limit of 2,4-DNP detection was 20 microM and the time of single analysis, the biosensor recovery included, was 30-50 min. In the continuous detection mode, the biosensor response was maintained at a stable level without biosensor inactivation for ten days. The biosensor can be used as an element of a complex analytical system for detecting nitroaromatic compounds in samples. 相似文献
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A. E. Kitova T. N. Kuvichkina A. Yu. Arinbasarova A. N. Reshetilov 《Applied Biochemistry and Microbiology》2004,40(3):258-261
The degradation of 2,4-dinitrophenol (2,4-DNP) by Rhodococcus erythropolis HL PM-1 was studied. The enzymes involved in 2,4-DNP degradation were inducible, and their resynthesis took place during the process. Cell immobilization by embedding into agar gels decreased the degrader activity. The maximum rates of 2,4-DNP degradation by free and immobilized cells were 10.0 and 5.4 nmol/min per mg cells, respectively. The concentration dependence of 2,4-DNP degradation was typical of substrate inhibition kinetics. The immobilized cells were used in a model reactor designed for 2,4-DNP biodegradation. Its maximum capacity was 0.45 nmol/min per mg cells at a volumetric flow rate of 20 h–1. The reactor operated for 14 days without losing capacity; its half-life equaled 16 days. 相似文献
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Development of Biosensors for Phenol Determination from Bacteria Found in Petroleum Fields of West Siberia 总被引:2,自引:0,他引:2
Makarenko A. A. Bezverbnaya I. P. Kosheleva I. A. Kuvichkina T. N. Ilyasov P. V. Reshetilov A. N. 《Applied Biochemistry and Microbiology》2002,38(1):23-27
Nine gram-negative bacterial strains, selected from 300 strains isolated from soils of the West Siberian petroliferous basin and growing on oil and oil products, consume phenol as a single carbon and energy source. The strains were used for the development of a sensor bioreceptor. The most active 32-I strain was shown to bear a plasmid responsible for phenol degradation. The plasmid-free derivative of this strain, 32-I-1, did not grow on phenol. The possibility of creating a model biosensor for phenol based on the plasmid-containing 32-I strain is considered. The detection limit for phenol was 5 M. The optimum conditions for the sensor operation are: pH 7.4, 35°C, and operation time 30 h. 相似文献
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Makarenko AA Arinbasarova AIu Kuvichkina TN Balashov SV Reshetilov AN 《Prikladnaia biokhimiia i mikrobiologiia》2005,41(5):521-524
Parameters of degradation of p-toluenesulfonate (TS) by free and agar-embedded Comamonas testosteroni BS1310 (pBS1010) cells were determined. The maximum rate of TS degradation was 25% lower in by immobilized than free cells, equaling 11 nmol x min(-1) x mg(-1) cells. Degradation of TS by both free and immobilized cells was associated with molecular oxygen consumption (molar ratio, 1 : 2). In a plug-flow reactor, the degradation rate was 10.4 nmol x min(-1) x mg(-1) cells. The results can be applied to designing reactors for TS degradation in sewage and developing biosensors. 相似文献
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Makarenko AA Bezverbnaia IP Kosheleva IA Kuvichkina TN Il'iasov PV Reshetilov AN 《Prikladnaia biokhimiia i mikrobiologiia》2002,38(1):29-34
Nine Gram-negative bacterial strains, selected from 300 strains isolated from soils of the West Siberian petroliferous basin and growing on oil and oil products, consume phenol as a single carbon and energy source. The strains were used for the development of a sensor bioreceptor. The most active 32-I strain was shown to bear a plasmid responsible for phenol degradation. The plasmid-free derivative of this strain, 32-I-1, did not grow on phenol. The possibility of creating a model biosensor for phenol based on the plasmid-containing 32-I strain is considered. The detection limit for phenol was 5 microM. The optimum conditions for the sensor operation are: pH 7.4, 35 degrees C, and operation time 30 h. 相似文献
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T. N. Kuvichkina E. N. Kaparullina N. V. Doronina Yu. A. Trotsenko A. N. Reshetilov 《Applied Biochemistry and Microbiology》2012,48(6):564-568
Enzymatic oxidative degradation of EDTA and EDTA complexes with metals has been investigated using immobilized cells of Chelativorans oligotrophicus LPM-4. A polarographic method, which makes it possible to register oxygen consumption by cells, has been used. For the first time, it has been indicated that the Cd-EDTA and Ni-EDTA complexes undergo degradation by the bacteria under study. 相似文献
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