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Rebecca C. Schreiber Stacey A. Vaccariello Kristen Boeshore Annette M. Shadiack Richard E. Zigmond 《Developmental neurobiology》2002,53(1):68-79
Transecting the axons of neurons in the adult superior cervical ganglion (SCG; axotomy) results in the survival of most postganglionic neurons, the influx of circulating monocytes, proliferation of satellite cells, and changes in neuronal gene expression. In contrast, transecting the afferent input to the SCG (decentralization) results in nerve terminal degeneration and elicits a different pattern of gene expression. We examined the effects of decentralization on macrophages in the SCG and compared the results to those previously obtained after axotomy. Monoclonal antibodies were used to identify infiltrating (ED1+) and resident (ED2+) macrophages, as well as macrophages expressing MHC class II molecules (OX6+). Normal ganglia contained ED2+ cells and OX6+ cells, but few infiltrating macrophages. After decentralization, the number of infiltrating ED1+ cells increased in the SCG to a density about twofold greater than that previously seen after axotomy. Both the densities of ED2+ and OX6+ cells were essentially unchanged after decentralization, though a large increase in OX6+ cells occurred after axotomy. Proliferation among the ganglion's total non‐neuronal cell population was examined and found to increase about twofold after decentralization and about fourfold after axotomy. Double‐labeling experiments indicated that some of these proliferating cells were macrophages. After both surgical procedures, the percentage of proliferating ED2+ macrophages increased, while neither procedure altered the proliferation of ED1+ macrophages. Axotomy, though not decentralization, increased the proliferation of OX6+ cells. Future studies must address what role(s) infiltrating and/or resident macrophages play in regions of decentralized and axotomized neurons and, if both are involved, whether they play distinct roles. © 2002 Wiley Periodicals, Inc. J Neurobiol 53: 68–79, 2002 相似文献
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A three-dimensional investigation of temporomandibular joint loading 总被引:15,自引:0,他引:15
A mathematical model of the muscle groups applied to the human mandible is developed to study the forces developed on the condyles during maximum unilateral occlusion. The results show that the reaction forces are in approximately a 2:1 ratio with the balancing side condyle carrying the greater load. Furthermore, the direction in which these condylar reactions occur is presented. 相似文献
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Dimensional analysis and scaling are related, semi-formal procedures for capturing the essential process(es) controlling the behaviour of a complex system, and for describing the functional relationships between them. The techniques involve the parameterization of natural processes, the identification of the temporal and spatial scales of variation of processes, and the evaluation of potential interactions between processes referenced to those scales using non-dimensional (scaled) parameters. Scaling approaches are increasingly being applied to a broad range of marine ecological problems, with the aims of assessing the relative importance of physical and biological parameters in controlling variation in process rates, and placing limits on the ability of one process to affect another. The value of the approach to coral reef research lies in the conceptualization of relationships between discipline-specific processes, and the evaluation of scale-dependent processes across the large range of spatial and temporal scales which pertain to coral reefs. Characteristic scales of physical, geological and biological processes exhibit different patterns of distribution along the temporal dimension. Scaling arguments based on examples from reef systems indicate that a large group of biological and biogeochemical processes are strongly influenced by hydrodynamic processe occuring at similar time scales within the range from about on hour to one year. We argue that scaling approaches to process-related problems are pre-requisite to interdisciplinary research on coral reefs. 相似文献
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Summary The nuclei of mesophyll cells of olive trees contain numerous sizeable crystalloid inclusions. Cytochemical examination using epoxy resin-embedded, semithin-sectioned tissue indicated the presence of proteins and oligoor polysaccharides in these inclusions. Their electron microscopical analysis revealed a crystalline substructure consisting of intersected subunits of high order. The spacing of the lattice fibrils and the angles of intersection were determined and used to establish a model of the unit cell of crystallization. It is suggested that the nuclear crystalloids of olive trees consist of glycoprotein molecules. They differ from the intranuclear crystalloids observed in other species predominantly in the high density of their subunit arrangement. 相似文献
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The complete amino acid sequence of human brain-derived acidic fibroblast growth factor 总被引:7,自引:0,他引:7
G Gimenez-Gallego G Conn V B Hatcher K A Thomas 《Biochemical and biophysical research communications》1986,138(2):611-617
Acidic fibroblast growth factor is a potent mitogen for a variety of cells in culture, including vascular endothelial cells, and is angiogenic in vivo. The complete amino acid sequence of human brain-derived acidic fibroblast growth factor has been determined from amino terminal sequence analysis and carboxypeptidase A digestion of the whole protein and sequence analyses of peptides generated by tryptic, Staphylococcus aureus V8 protease and cyanogen bromide cleavages. A potential Asn-Gly-Ser glycosylation sequence is present in the human protein. The complete amino acid sequence is compared to that of the equivalent protein purified from bovine brain. 相似文献
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V B Hatcher N Fadl-Allah M A Levitt A Brown S S Margossian P B Gordon 《Journal of cellular physiology》1986,128(3):353-361
Human endothelial cells release components into the growth medium that stimulate cell-substratum adhesion. Several macromolecular components were isolated by ultracentrifugation of the endothelial cell conditioned medium. The components were heterogeneous, consisting of several sizes when examined by sedimentation velocity and gel filtration. When the extracellular components were evaluated by electron microscopy, structurally discrete particles were observed. The extracellular components and the complexes mediated cell-substratum adhesion to both human umbilical and arterial endothelial cells. The majority of the extracellular components that promote endothelial cell adhesion were pelleted by ultracentrifugation. Although the complexes contained fibronectin, antibodies to fibronectin did not inhibit cell adhesion to the complexes. Significant inhibition of endothelial cell adhesion was observed in the presence of heparin and heparan sulfate. The supernatant fraction following ultracentrifugation of the growth medium contained a component that suppressed endothelial cell adhesion to culture dishes coated with fibronectin, type I collagen, and endothelial cell complexes. SDS-polyacrylamide gel electrophoresis indicated that the complexes contained several components, and the majority of the large-molecular-weight components were pelleted by ultracentrifugation. The conditioned medium from human endothelial cells contains specific complexes that promote cell-substratum adhesion and components that suppress cell-substratum adhesion. 相似文献