排序方式: 共有19条查询结果,搜索用时 0 毫秒
1.
KB Cullberg T Christiansen SK Paulsen JM Bruun SB Pedersen B Richelsen 《Obesity (Silver Spring, Md.)》2013,21(3):454-460
Background:
Vascular growth is a prerequisite for adipose tissue (AT) development and expansion. Some AT cytokines and hormones have effects on vascular development, like vascular endothelial growth factor (VEGF‐A), angiopoietin (ANG‐1), ANG‐2 and angiopoietin‐like protein‐4 (ANGPTL‐4).Methods:
In this study, the independent and combined effects of diet‐induced weight loss and exercise on AT gene expression and proteins levels of those angiogenic factors were investigated. Seventy‐nine obese males and females were randomized to: 1. Exercise‐only (EXO; 12‐weeks exercise without diet‐restriction), 2. Hypocaloric diet (DIO; 8‐weeks very low energy diet (VLED) + 4‐weeks weight maintenance diet) and 3. Hypocaloric diet and exercise (DEX; 8‐weeks VLED + 4‐weeks weight maintenance diet combined with exercise throughout the 12 weeks). Blood samples and fat biopsies were taken before and after the intervention.Results:
Weight loss was 3.5 kg in the EXO group and 12.3 kg in the DIO and DEX groups. VEGF‐A protein was non‐significantly reduced in the weight loss groups. ANG‐1 protein levels were significantly reduced 22‐25% after all three interventions (P < 0.01). The ANG‐1/ANG‐2 ratio was also decreased in all three groups (P < 0.05) by 27‐38%. ANGPTL‐4 was increased in the EXO group (15%, P < 0.05) and 9% (P < 0.05) in the DIO group. VEGF‐A, ANG‐1, and ANGPTL‐4 were all expressed in human AT, but only ANGPTL‐4 was influenced by the interventions.Conclusions:
Our data show that serum VEGF‐A, ANG‐1, ANG‐2, and ANGPTL‐4 levels are influenced by weight changes, indicating the involvement of these factors in the obese state. Moreover, it was found that weight loss generally was associated with a reduced angiogenic activity in the circulation. 相似文献2.
3.
4.
KB Gudmundsdóttir S Sigurdarson J Kristinsson T Eiríksson T Jóhannesson 《Acta veterinaria Scandinavica》2006,48(1):16-5
This study was undertaken in order to examine whether any connection existed between the amounts of iron in forage and the
sporadic occurrence of scrapie observed in certain parts of Iceland. As iron and manganese are considered antagonistic in
plants, calculation of the Fe/Mn ratios was also included by using results from Mn determination earlier performed in the
same samples. Forage samples (n = 170) from the summer harvests of 2001–2003, were collected from 47 farms for iron and manganese
analysis. The farms were divided into four categories: 1. Scrapie-free farms in scrapie-free areas (n = 9); 2. Scrapie-free farms in scrapie-afflicted areas (n = 17); 3. Scrapie-prone farms (earlier scrapie-afflicted, restocked farms) (n = 12); 4. Scrapie-afflicted farms (n = 9). Farms in categories 1 and 2 are collectively referred to as scrapie-free farms. The mean iron concentration in forage samples from scrapie-afflicted farms was significantly higher than in forage samples
from farms in the other scrapie categories (P = 0.001). The mean Fe/Mn ratio in forage from scrapie-afflicted farms was significantly
higher than in forage from scrapie-free and scrapie-prone farms (P < 0.001). The results indicated relative dominance of iron
over manganese in forage from scrapie-afflicted farms as compared to farms in the other categories. Thus thorough knowledge
of iron, along with manganese, in soil and vegetation on sheep farms could be a pivot in studies on sporadic scrapie. 相似文献
5.
Scanning electron microscopy and gramicidin patch clamp recordings of microvillous receptor neurons dissociated from the rat vomeronasal organ 总被引:2,自引:1,他引:1
Vomeronasal organs from female rats were dissociated and isolated
microvillous receptor neurons were studied. The isolated receptor neurons
kept the typical bipolar shape which they have in situ as observed by
scanning electron microscopy. We applied the perforated patch-clamp
technique using the cation-selective ionophore gramicidin on freshly
isolated and well differentiated receptor neurons. The mean resting
potential was -58+/-14 mV (n=39). The contribution of the sodium pump
current to the resting potential was demonstrated by lowering the K+
concentration in the bath or by application of 100 microM dihydro-ouabain.
The input resistance was in the range of 1-6 GOmega and depolarizing
current pulses of a few pA were sufficient to trigger overshooting action
potentials. In voltage clamp conditions a fast transient sodium inward
current and a sustained outward potassium current were activated by
membrane depolarization. These observations indicate that freshly isolated
vomeronasal receptor neurons of rats can be recorded, using gramicidin,
with little modification of the intracellular content. Their
electrophysiological properties are very similar to those observed in situ.
Four out of eight female vomeronasal receptor cells were depolarized by
diluted rat male urine.
相似文献
6.
Pious Thomas Chinnaian Goplakrishnan Manem Krishnareddy 《Plant Cell, Tissue and Organ Culture》2011,105(3):423-429
This study was undertaken to ascertain if the soft rot inciting Pectobacterium carotovorum/Erwinia carotovora would pass through the micropropagated bananas as a latent pathogen and cause disease during or post acclimatization. In
vitro cultures of ‘Grand Naine’ were exposed to the pathogen by providing 100 μl of inoculum (0.001–1.0 at OD600 nm) at the lower leaf axil. These cultures showed a gradual development of soft rot symptoms coupled with obvious bacterial
colony growth on banana proliferation medium and consequent plant mortality within a month irrespective of the inoculum level
employed. Plants carried forward to acclimatization following inoculation in vitro failed to establish ex vitro. Monitoring
the normal field-grown suckers at culture initiation through PCR screening employing soft rot Erwinia primers did not show the amplification of the 119-bp fragment as seen with the pure cultures of pathogen. Further testing
of micropropagated banana plants through soil inoculation, in vitro culturing and PCR screening ruled out the possibility
of the pathogen surviving in micropropagated stocks in latent form as the organism outgrew and killed the cultures. It emerged
that the infection possibly takes place in the nursery. This information will be of particular value for the plant tissue
culture industry, plant pathologists and quarantine agencies. 相似文献
7.
Qi Zhang Gang Hua Krishnareddy Bayyareddy Michael J. Adang 《Insect biochemistry and molecular biology》2013,43(10):907-915
Bacillus thuringiensis subsp. jegathesan produces Cry11Ba crystal protein with high toxicity to mosquito larvae. The Cry11Ba toxicity is dependent on its receptors on mosquito larval midgut epithelial cells. Previously, a cadherin-like protein (AgCad2), aminopeptidase (AgAPN2) and alkaline phosphatase (AgALP1) were reported to be involved in regulation of Cry11Ba toxicity on Anopheles gambiae larvae. Here, the cDNAs encoding α-amylase (AgAmy1) and α-glucosidase (Agm3) were cloned from A. gambiae larva midgut. Both are glycophosphatidylinositol (GPI) anchored proteins on brush border membranes (BBMV). Immunohistochemistry revealed their localization on different regions of the larval midgut. AgAmy1 and Agm3 bound Cry11Ba with high affinity, 37.6 nM and 21.1 nM respectively. Cry11Ba toxicity against A. gambiae larvae was neutralized by both AgAmy1 and Agm3. The results provide evidence that both AgAmy1 and Agm3 function as receptors of Cry11Ba in A. gambiae. 相似文献
8.
Stéphane L. Benoit Krishnareddy Bayyareddy Manish Mahawar Joshua S. Sharp Robert J. Maier 《Journal of bacteriology》2013,195(23):5396-5401
Protein exposure to oxidants such as HOCl leads to formation of methionine sulfoxide (MetSO) residues, which can be repaired by methionine sulfoxide reductase (Msr). A Helicobacter pylori
msr strain was more sensitive to HOCl-mediated killing than the parent. Because of its abundance in H. pylori and its high methionine content, alkyl hydroperoxide reductase C (AhpC) was hypothesized to be prone to methionine oxidation. AhpC was expressed as a recombinant protein in Escherichia coli. AhpC activity was abolished by HOCl, while all six methionine residues of the enzyme were fully to partially oxidized. Upon incubation with a Msr repair mixture, AhpC activity was restored to nonoxidized levels and the MetSO residues were repaired to methionine, albeit to different degrees. The two most highly oxidized and then Msr-repaired methionine residues in AhpC, Met101 and Met133, were replaced with isoleucine residues by site-directed mutagenesis, either individually or together. E. coli cells expressing variant versions were more sensitive to t-butyl hydroperoxide than cells expressing native protein, and purified AhpC variant proteins had 5% to 39% of the native enzyme activity. Variant proteins were still able to oligomerize like the native version, and circular dichroism (CD) spectra of variant proteins revealed no significant change in AhpC conformation, indicating that the loss of activity in these variants was not related to major structural alterations. Our results suggest that both Met101 and Met133 residues are important for AhpC catalytic activity and that their integrity relies on the presence of a functional Msr. 相似文献
9.