全文获取类型
收费全文 | 5507篇 |
免费 | 271篇 |
国内免费 | 3篇 |
出版年
2023年 | 12篇 |
2022年 | 16篇 |
2021年 | 67篇 |
2020年 | 27篇 |
2019年 | 59篇 |
2018年 | 95篇 |
2017年 | 59篇 |
2016年 | 105篇 |
2015年 | 153篇 |
2014年 | 194篇 |
2013年 | 365篇 |
2012年 | 351篇 |
2011年 | 322篇 |
2010年 | 213篇 |
2009年 | 218篇 |
2008年 | 346篇 |
2007年 | 340篇 |
2006年 | 329篇 |
2005年 | 317篇 |
2004年 | 345篇 |
2003年 | 331篇 |
2002年 | 313篇 |
2001年 | 78篇 |
2000年 | 82篇 |
1999年 | 80篇 |
1998年 | 73篇 |
1997年 | 59篇 |
1996年 | 45篇 |
1995年 | 53篇 |
1994年 | 42篇 |
1993年 | 38篇 |
1992年 | 59篇 |
1991年 | 66篇 |
1990年 | 58篇 |
1989年 | 64篇 |
1988年 | 42篇 |
1987年 | 32篇 |
1986年 | 37篇 |
1985年 | 33篇 |
1984年 | 30篇 |
1983年 | 29篇 |
1982年 | 24篇 |
1981年 | 17篇 |
1980年 | 16篇 |
1978年 | 12篇 |
1977年 | 13篇 |
1976年 | 17篇 |
1975年 | 20篇 |
1974年 | 15篇 |
1973年 | 17篇 |
排序方式: 共有5781条查询结果,搜索用时 125 毫秒
1.
2.
3.
T Nimura M Okada M Shimizu M Kawabe T Itoh M Iwasaki H Kimura T Takeuchi 《The Annals of physiological anthropology》1990,9(4):329-333
Motorcyclists who work in some offices sometimes complained of coldness, pain and numbness of upper limbs. We studied how to discriminate between vibration syndrome and local fatigue of the motorcyclists. Subjects are 42 motorcyclists of an office in Aichi prefecture. 25 of them held several letters in their left hand when they delivered the letters. They complained of coldness, pain and numbness in the left upper limbs more than in the right limbs (p less than 0.01). We think that it is the local fatigue rather than the disorder of vibration syndrome that causes such symptoms. So it is very important to recognize the existence of local fatigue in order to know how to discriminate between vibration syndrome and local fatigue of the motorcyclists. 相似文献
4.
Structure and expression of cDNA for an inhibitor of blood coagulation isolated from human placenta: a new lipocortin-like protein 总被引:10,自引:0,他引:10
A Iwasaki M Suda H Nakao T Nagoya Y Saino K Arai T Mizoguchi F Sato H Yoshizaki M Hirata 《Journal of biochemistry》1987,102(5):1261-1273
An inhibitor of blood coagulation, a new protein with an apparent molecular weight of 34,000 and an isoelectric point of 4.9, was purified from human placental tissue by EDTA extraction. Five cDNA clones were isolated from the human placental lambda gt11 cDNA library using the mouse monoclonal antibody raised against the coagulation inhibitor as the probe. The longest insert consists of 1,566 nucleotides, and contains 960 nucleotides entirely encoding the 320 amino acids of the inhibitor, and a poly A tail. The deduced amino acid sequence was corroborated by chemical analyses of the protein. The entire amino acid sequence shows homology to those of lipocortin I, lipocortin II, and endonexin-related proteins. The cDNA for the inhibitor was expressed in Escherichia coli under the regulation of the trc promotor of the plasmid pKK233-2. The resulting recombinant protein manifested inhibitory activities against both blood coagulation and phospholipase A2 activity, as did the coagulation inhibitor isolated from human placenta. 相似文献
5.
Hirotaka Yamamoto Hidehiko Konno Teiji Yamamoto Kitae Ito Michinao Mizugaki Yuzo Iwasaki 《Journal of neurochemistry》1987,49(2):603-609
Glutamine synthetase (GS) isolated from human brain formed a single band on sodium dodecyl sulfate-polyacrylamide gel with a molecular weight of 44,000. The enzyme had a specific activity of 179.2 U/mg protein when assayed by measuring the rate of the formation of gamma-glutamylhydroxamate using hydroxylamine as a substrate. In the presence of manganese ions, the relative activity of human brain GS was much lower than that of the sheep brain enzyme. The suppression of activity by increasing the ADP concentration, however, was less marked in the human enzyme than that in the sheep enzyme. Antibodies were raised in rabbits against the purified enzyme. The double-immunodiffusion technique disclosed cross-reactivities among GSs isolated from human, sheep, and rat brains, but the enzymes were not immunologically identical. Immunohistochemically, GS was localized in the cytoplasm of astrocytes in the human and rat brains and in pericentral hepatocytes of the liver. 相似文献
6.
7.
8.
Hiroshi Iwasaki Toshikazu Shiba Atsuo Nakata Hideo Shinagawa 《Molecular & general genetics : MGG》1989,219(1-2):328-331
Summary The ruv operon of Escherichia coli consists of two genes, orfl1 and ruv, which encode 22 and 37 kilodalton proteins, respectively, and are regulated by the SOS system. Although the distal gene, ruv, is known to be involved in DNA repair, the function of orf1 has not been studied. To examine whether orf1 is also involved in DNA repair, we constructed a strain with a deletion of the entire ruv operon. The strain was sensitive to UV even after introduction of low copy number plasmids carrying either orf1 or ruv, but UV resistance was restored by introduction of a plasmid carrying both orfl and ruv. These results suggest that orf1 as well as ruv is involved in DNA repair. Therefore, orf1 and ruv should be renamed ruvA and ruvB, respectively. 相似文献
9.
Atsuko Matsuoka Akiko Hirosawa Shinasku Natori Shigeo Iwasaki Toshio Sofuni Motoi Ishidate Jr. 《Mutation research》1989,215(2):179-185
The chromosomal aberration test using a Chinese hamster lung cell line (CHL) was carried out on ptaquiloside and its related compounds, hypoloside B, hypoloside C, illudin M and illudin S. Ptaquiloside induced chromosomal aberrations at doses as low as 4.5 μg/ml (0.0113 mM). The clastogenic effect was ph-dependent. The same activity was observed at a 90-fold higher dose at pH 5.3 in the culture medium compared with the activity at pH 74. or pH 8.0. Both hypoloside B and hypoloside C were also clastogenic at almost the same dose levels as that of ptaquiloside. Illudin M and illudin S were also potet clastogens and induced aberrations at much lower doses than ptaquiloside. These results suggest that the clastogenic effect is involved in the mechanism of carcinogenic potency of ptaquiloside in animals. 相似文献
10.