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Tsyganov V. E.; Pavlova Z. B.; Kravchenko L. V.; Rozov S. M.; Borisov A. Y.; Lutova L. A.; Tikhonovich I. A. 《Annals of botany》2000,86(5):975-981
Using ethyl methane sulfonate (EMS) treatment of the seeds ofline SGE, a new mutant of pea (Pisum sativum L.) with alterationsin root development was obtained. The mutant phenotype dependson the density of the growth substrate: on sand (a high densitysubstrate) the mutant forms a small compact curly root systemwhereas on vermiculite (a low density substrate) differencesbetween the root systems of the mutant and wild type plantsare less pronounced. Genetic analysis revealed that the mutantcarries a mutation in a new pea gene designedcrt (curly roots).Gene crt has been localized in pea linkage group V. The mutantline named SGEcrt showed increased sensitivity to exogenousauxin and an increased concentration of endogenous indole-3-aceticacid (IAA) in comparison with the wild type line SGE. Copyright2000 Annals of Botany Company Pisum sativum L., root development, garden pea mutant, curly roots, auxin, environmental stimulus response 相似文献
3.
Farid M. Ibatullin Alexander M. Golubev Leonid M. Firsov Kirill N. Neustroev 《Glycoconjugate journal》1993,10(3):214-218
The present work investigated the possibility of cleavage of -linkages between mannose or galactose and serine/threonine residues by -mannosidase and -galactosidase. The study was carried out initially with model synthetic compounds imitating theO-glycosidic bond in glycoproteins, and further with glucoamylase. It was shown that -mannosidase and -galactosidase can hydrolyse these linkages after proteolytic digestion of glucosamylase. 相似文献
4.
Irina Zaitseva Vjacheslav Zaitsev Graeme Card Kirill Moshkov Benjamin Bax Adam Ralph Peter Lindley 《Journal of biological inorganic chemistry》1996,1(1):15-23
The X-ray structure of human serum ceruloplasmin has been solved at a resolution of 3.1?Å. The structure reveals that the molecule is comprised of six plastocyanin-type domains arranged in a triangular array. There are six copper atoms; three form a trinuclear cluster sited at the interface of domains 1 and 6, and there are three mononuclear sites in domains 2, 4 and 6. Each of the mononuclear coppers is coordinated to a cysteine and two histidine residues, and those in domains 4 and 6 also coordinate to a methionine residue; in domain 2, the methionine is replaced by a leucine residue which may form van der Waals type contacts with the copper. The trinuclear centre and the mononuclear copper in domain 6 form a cluster essentially the same as that found in ascorbate oxidase, strongly suggesting an oxidase role for ceruloplasmin in the plasma. 相似文献
5.
Hummel Herman Bogaards Roelof Bek Tatiana Polishchuk Lennard Amiard-Triquet Claude Bachelet Guy Desprez Michel Strelkov Peter Sukhotin Alex Naumov Andrei Dahle Salve Denisenko Stanislav Gantsevich Michael Sokolov Kirill Wolf Lein de 《Hydrobiologia》1997,355(1-3):127-138
The stress sensitivity, determined in copper exposureexperiments and in survival in air tests, and thegenetic structure, measured by means of isoenzymeelectrophoresis, were assessed in populations of theBaltic clam Macoma balthica (L.) from itssouthern to its northern distribution limit, in orderto test the hypotheses that near the distributionlimit the clams would be more stress sensitive andwould have a lower genetic variability. Thepopulations in west and north Europe show a stronggenetic resemblance. The populations in the sub-ArcticWhite Sea are genetically slightly different, and showa low stress sensitivity. The populations in theArctic Pechora Sea are genetically very distant fromthe other populations, and show the lowest stresssensitivity. Near the southern distribution limit, inagreement with the hypotheses, genetic variability islow and stress sensitivity high. On the other hand, incontrast to expectation, near the northerndistribution limit, in the populations of the PechoraSea, the genetic variability was higher, thus notreduced, and the stress sensitivity was low comparedto all other populations. Yet, it remains a questionif such is due to gradual physiologicalacclimatization (and ongoing differential selection)or to genetic adaptation. 相似文献
6.
Dmitry E. Nolde Alexander G. Sobol Kirill A. Pluzhnikov Eugene V. Grishin Alexander S. Arseniev 《Journal of biomolecular NMR》1995,5(1):1-13
Summary Two-dimensional 1H NMR techniques were used to determine the spatial structure of ectatomin, a toxin from the venom of the ant Ectatomma tuberculatum. Nearly complete proton resonance assignments for two chains of ectatomin (37 and 34 amino acid residues, respectively) were
obtained using 2D TOCSY, DQF-COSY and NOESY experiments. The cross-peak volumes in NOESY spectra were used to define the local
structure of the protein and generate accurate proton-proton distance constraints employing the MARDIGRAS program. Disulfide
bonds were located by analyzing the global fold of ectatomin, calculated with the distance geometry program DIANA. These data,
combined with data on the rate of exchange of amide protons with deuterium, were used to obtain a final set of 20 structures
by DIANA. These structures were refined by unrestrained energy minimization using the CHARMm program. The resulting rms deviations
over 20 structures (excluding the mobile N- and C-termini of each chain) are 0.75 ? for backbone heavy atoms, and 1.25 ? for
all heavy atoms. The conformations of the two chains are similar. Each chain consists of two α-helices and a hinge region
of four residues; this forms a hairpin structure which is stabilized by disulfide bridges. The hinge regions of the two chains
are connected together by a third disulfide bridge. Thus, ectatomin forms a four-α-helical bundle structure. 相似文献
7.
It was shown earlier that during collisions bacterial population waves may either penetrate one another or stop. In this communication, the mechanism of these two interaction modes is considered in detail. It is shown on the basis of theoretical and experimental results that this interaction is a graphic example confirming one of the characteristic properties of waves in cross-diffusion systems. 相似文献
8.
Ilya A. Udalov Won Je Lee Kirill Lotonin Alexey Smirnov 《The Journal of eukaryotic microbiology》2020,67(1):132-139
A new marine species of naked lobose amoebae Pseudoparamoeba garorimi n. sp. (Amoebozoa, Dactylopodida) isolated from intertidal marine sediments of Garorim Bay, Korea was studied with light and transmission electron microscopy. This species has a typical set of morphological characters for a genus including the shape of the locomotive form, type of subpseudopodia and the tendency to form the single long waving pseudopodium in locomotion. Furthermore, it has the same cell surface structures as were described for the type species, Pseudoparamoeba pagei: blister‐like glycostyles with hexagonal base and dome‐shaped apex; besides, cell surface bears hair‐like outgrowths. The new species described here lacks clear morphological distinctions from the two other Pseudoparamoeba species, but has considerable differences in the 18S rDNA and COX1 gene sequences. Phylogenetic analysis based on 18S rDNA placed P. garorimi n. sp. at the base of the Pseudoparamoeba clade with high PP/BS support. The level of COX1 sequence divergence was 22% between P. garorimi n. sp. and P. pagei and 25% between P. garorimi n. sp. and P. microlepis. Pseudoparamoeba species are hardly distinguishable by morphology alone, but display clear differences in 18S rDNA and COX1 gene sequences. 相似文献
9.
Luba A. Astakhova Alexander Yu. Rotov Roman V. Cherbunin Arsenii A. Goriachenkov Kirill V. Kavokin Michael L. Firsov Nikita Chernetsov 《Proceedings. Biological sciences / The Royal Society》2020,287(1940)
Migratory birds are known to be sensitive to external magnetic field (MF). Much indirect evidence suggests that the avian magnetic compass is localized in the retina. Previously, we showed that changes in the MF direction could modulate retinal responses in pigeons. In the present study, we performed similar experiments using the traditional model animal to study the magnetic compass, European robins. The photoresponses of isolated retina were recorded using ex vivo electroretinography (ERG). Blue- and red-light stimuli were applied under an MF with the natural intensity and two MF directions, when the angle between the plane of the retina and the field lines was 0° and 90°, respectively. The results were separately analysed for four quadrants of the retina. A comparison of the amplitudes of the a- and b-waves of the ERG responses to blue stimuli under the two MF directions revealed a small but significant difference in a- but not b-waves, and in only one (nasal) quadrant of the retina. The amplitudes of both the a- and b-waves of the ERG responses to red stimuli did not show significant effects of the MF direction. Thus, changes in the external MF modulate the European robin retinal responses to blue flashes, but not to red flashes. This result is in a good agreement with behavioural data showing the successful orientation of birds in an MF under blue, but not under red illumination. 相似文献
10.
Aleksandr A Rubel Tatyana A Ryzhova Kirill S Antonets Yury O Chernoff Alexey P Galkin 《朊病毒》2013,7(6):469-476
Alzheimer disease is associated with the accumulation of oligomeric amyloid β peptide (Aβ), accompanied by synaptic dysfunction and neuronal death. Polymeric form of prion protein (PrP), PrPSc, is implicated in transmissible spongiform encephalopathies (TSEs). Recently, it was shown that the monomeric cellular form of PrP (PrPC), located on the neuron surface, binds Aβ oligomers (and possibly other β-rich conformers) via the PrP23–27 and PrP90–110 segments, acting as Aβ receptor. On the other hand, PrPSc polymers efficiently bind to Aβ monomers and accelerate their oligomerization. To identify specific PrP sequences that are essential for the interaction between PrP polymers and Aβ peptide, we have co-expressed Aβ and PrP (or its shortened derivatives), fused to different fluorophores, in the yeast cell. Our data show that the 90–110 and 28–89 regions of PrP control the binding of proteinase-resistant PrP polymers to the Aβ peptide, whereas the 23–27 segment of PrP is dispensable for this interaction. This indicates that the set of PrP fragments involved in the interaction with Aβ depends on PrP conformational state. 相似文献