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1.
2.
Wakae Fujimaki Keiko Baba Katsunori Tatara Ryohji Umezu Sanji Kusakawa Yasushige Mashima 《Human genetics》1987,76(3):302-302
Summary A case of ring chromosome 15 passed on to the index patient's two children is reported, and possible reasons for the infrequent records of inheritance of ring chromosome are suggested. 相似文献
3.
Norio Masui Yumie Takagi Tetsu Nishikawa Makoto Yanabe Masato Nose Katsunori Sato 《Experimental Animals》2002,51(5):501-503
A new polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was developed for genetic typing of the mouse Tnfsf6gld mutation. An artificial restriction site was introduced to the mouse Tnfsf6gld mutation by PCR amplification using a modified primer. The three genotypes of the Tnfsf6 locus (Tnfsf6gld/Tnfsf6gld, Tnfsf6gld/+, and +Tnfsf6-gld/+Tnfsf6-gld) could be distinguished clearly and easily. This PCR-RFLP analysis was found to be useful for the identification of the mouse Tnfsf6gld mutation. 相似文献
4.
Transmembrane diffusion of hydrophobic antimicrobial agents and cell surface hydrophobicity in Bacteroides fragilis 总被引:1,自引:0,他引:1
The transmembrane diffusion of hydrophobic antimicrobial agents, e.g. lincomycin and clindamycin, was examined in Bacteroides fragilis which is sensitive to these agents. The results showed that these agents penetrate efficiently through the outer membrane. Cell surface hydrophobicity measured by the partition assay between water and p-xylene revealed that the cell surface of B. fragilis is more hydrophobic than that of Salmonella typhimurium or Pseudomonas aeruginosa. Furthermore, treatment with low concentrations of surfactant caused cell lysis. These results suggest that the cell surface hydrophobicity in B. fragilis plays an important role in the efficient transmembrane penetration of hydrophobic compounds. This efficiency explains the susceptibility of B. fragilis to hydrophobic antimicrobial agents. 相似文献
5.
6.
In Dunaliella tertiolecta, D. bioculata and D. viridis the activitiesof phosphoenolpyruvate carboxylase and carbonic anhydrase werehigher in the cells grown in ordinary air (low-CO2 cells) thanin those grown in air enriched with 15% CO2 (high-CO2cells), whereas in Porphyridium cruentum R-1 there was no differencein phosphoenolpyruvate carboxylase activity between these twotypes of cells. Apparent Km(NaHCO3) values for photosynthesisin low-CO2 cells of all species tested were smaller than thosein high-CO2 cells. Most of the 14C was incorporated into 3-phosphoglycerate,sugar mono- and di-phosphates during the initial periods ofphotosynthetic NaH14CO3 indicating that both types of cellsin D. tertiolecta are C3 plants. (Received May 27, 1985; Accepted June 25, 1985) 相似文献
7.
Total starch, amylose content and amylose-included lipid phosphorus and lysophosphatidylcholine (LPC) were measured in normal Glacier (G) and Hi Amylose Glacier (HA) barley varieties during germination. From days three to six, alkaline and acidic lysophospholipase (LPL) activities in the starchy endosperm were measured and the distribution of these activities between a soluble and particulate form determined. During germination the amylose content of the starches increases as the total starch levels decline. The starch-bound LPC and lipid phosphorus disappear at the same rate between days three and six in both barley varieties, indicating no discrimination among the different lipid-included amylose population for degradation. However, both lipid phosphorus and LPC disappear more rapidly in the G than in the HA variety. This is presumably due to the slightly larger content of LPC per mg amylose of the G than of the HA variety, equivalent to 134 and 150 anhydroglucose residues per lipid molecule in G and HA, respectively. There is no increase in starch-bound lipid phosphorus or LPC expressed as nmol of phosphorus or LPC per mg amylose as amylose content declines, indicating no selective resistance of lipid-included amylose to degradation. The alkaline and acidic LPC activities in each variety increase 2–4-fold between days four and five. In both varieties ca 30% of the acidic LPL and ca 50–60% of the alkaline LPL is particulate from days three to six. No correlation can be made between the content of amylose or amylose-included lipid and particulate LPL activity. However, the possibility that particulate LPL activity is associated with specific populations of residual amylose-included lipid molecules cannot be excluded. 相似文献
8.
Katsunori Kohda Yuji Tsuji Masahiro Takagi Tadayuki Imanaka 《Biotechnology letters》1996,18(9):1061-1066
Genes homologous to groES and groEL, which are recognized as molecular chaperone genes, from Bacillus stearothermophilus SIC1 were cloned and sequenced. By addition of GroES, GroEL and ATP in vitro, remaning activity of the alcohol dehydrogenase from Saccharomyces cerevisiae after heat treatment at 50°C for 6 min was improved from 55% to 90%. Furthermore, even though inclusion bodies were formed when a single chain Fv(sFv) was expressed in E. coli cells, during in vivo coexpression with molecular chaperone, a significant amount of the antibody protein could be recovered from the soluble fraction. 相似文献
9.
Katsunori Mizoguchi Masatomo Morita Curt R. Fischer Masatoshi Yoichi Yasunori Tanji Hajime Unno 《Applied microbiology》2003,69(1):170-176
The interaction between Escherichia coli O157:H7 and its specific bacteriophage PP01 was investigated in chemostat continuous culture. Following the addition of bacteriophage PP01, E. coli O157:H7 cell lysis was observed by over 4 orders of magnitude at a dilution rate of 0.876 h−1 and by 3 orders of magnitude at a lower dilution rate (0.327 h−1). However, the appearance of a series of phage-resistant E. coli isolates, which showed a low efficiency of plating against bacteriophage PP01, led to an increase in the cell concentration in the culture. The colony shape, outer membrane protein expression, and lipopolysaccharide production of each escape mutant were compared. Cessation of major outer membrane protein OmpC production and alteration of lipopolysaccharide composition enabled E. coli O157:H7 to escape PP01 infection. One of the escape mutants of E. coli O157:H7 which formed a mucoid colony (Mu) on Luria-Bertani agar appeared 56 h postincubation at a dilution rate of 0.867 h−1 and persisted until the end of the experiment (~200 h). Mu mutant cells could coexist with bacteriophage PP01 in batch culture. Concentrations of the Mu cells and bacteriophage PP01 increased together. The appearance of mutant phage, which showed a different host range among the O157:H7 escape mutants than wild-type PP01, was also detected in the chemostat culture. Thus, coevolution of phage and E. coli O157:H7 proceeded as a mutual arms race in chemostat continuous culture. 相似文献
10.
SGLT1, an isoform of Na+-dependent glucose transporters, is localized at the apical plasma membrane in the epithelial cells of the small intestine
and the kidney. In the present study we examined its location in SGLT1 cDNA-transfected MDCK cells, which form an epithelial
sheet connected by tight junctions in culture. Formation of tight junctions was monitored by staining for occludin, an integral
tight junction protein. In the cells demarcated by an uninterrupted occludin meshwork, SGLT1 was specifically localized at
the apical plasma membrane, showing that SGLT1 has a signal to accomplish this restricted localization. In the cells with
little or no occludin accumulation in the tight junction, however, SGLT1 was present along the entire aspect of the plasma
membrane. Similar distribution of SGLT1 was observed in the cells as long as the occludin meshwork remained incomplete. These
observations sugget that apical localization of SGLT1 occurs upon the completion of the uninterrupted meshwork of tight junctions. 相似文献